The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
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Effects of Gwaruhaengryeon-hwan on COPD and Particulate Matter Induced Lung Injury on a Mouse Model

만성폐쇄성폐질환 및 미세먼지 유발 폐손상 동물모델에서 과루행련환의 효과

  • Lee, Chul-wha (Dept. of Internal Medicine, College of Korean Medicine, Dae-Jeon University) ;
  • Yang, Won-kyung (Dept. of Internal Medicine, College of Korean Medicine, Dae-Jeon University) ;
  • Lyu, Yee-ran (Dept. of Internal Medicine, College of Korean Medicine, Dae-Jeon University) ;
  • Kim, Seung-hyeong (Institute of Traditional Medicine and Bioscience, Dae-Jeon University) ;
  • Park, Yang-chun (Dept. of Internal Medicine, College of Korean Medicine, Dae-Jeon University)
  • 이철화 (대전대학교 한의과대학 내과학교실) ;
  • 양원경 (대전대학교 한의과대학 내과학교실) ;
  • 유이란 (대전대학교 한의과대학 내과학교실) ;
  • 김승형 (대전대학교 동서생명과학연구원) ;
  • 박양춘 (대전대학교 한의과대학 내과학교실)
  • Received : 2017.05.31
  • Accepted : 2017.06.29
  • Published : 2017.06.30
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Abstract

Objective: This study aimed to use a mouse model to evaluate the effects of Gwaruhaengryeon-hwan (GHH) on chronic obstructive pulmonary disease (COPD) and particulate matter induced lung injury. Materials and Methods: The study was carried out in two ways (in vitro, in vivo). In vitro RAW 264.7 cells (mouse macrophage) were used and analyzed by flow cytometry, ELISA. In vivo lipopolysaccharide (LPS) and cigarette smoke solution (CSS), or coal, fly ash, diesel exhaust particle (CFD) challenged mice were used and its BALF was analyzed by ELISA, lung tissue by real-time PCR. Results: In vitro, GHH maintained an 80-100% rate of viability. So cytotoxicity was not shown. In the ELISA analysis with RAW 264.7 cells, GHH significantly decreased NO over $30{\mu}g/ml$. In the ELISA analysis, GHH significantly decreased $TNF-{\alpha}$, IL-6 over $300{\mu}g/ml$. In the COPD model, the GHH 200 mg/kg dosage group, the application of GHH significantly decreased the increasing of neutrophils, $TNF-{\alpha}$, IL-17A, MIP2, CXCL-1 in BALF, $TNF-{\alpha}$, $IL-1{\beta}$ mRNA expression in lung tissue and histological lung injury. In the CFD induced lung injury model, the GHH 200 mg/kg dosage group, the application of GHH significantly decreased the increase of neutrophils, $TNF-{\alpha}$, IL-17A, MIP2, CXCL-1 in BALF, MUC5AC, $TGF-{\beta}$ mRNA expression in lung tissue and histological lung injury. Conclusion: This study suggests the usability of GHH for COPD patients by controlling lung tissue injury.

Keywords

References

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