Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
권호 표지
DOI QR코드

DOI QR Code

Antimicrobial activity and protective effect of Geranium thunbergii against oxidative DNA damage via antioxidant effect

현초의 항산화 활성에 의한 산화적 DNA 손상 보호효과 및 항균활성

  • Kwon, Tae-Hyung (Eco-friendly Certification Support Center, Chuncheon Bioindustry Foundation) ;
  • Lee, Su-Jin (Department of Herbal Medicine Resource, Kangwon National University) ;
  • Park, Jae-Ho (Department of medicinal plant science, Jungwon University) ;
  • Kim, Taewan (Department of Food Science and Biotechnology, Andong National University) ;
  • Park, Jung-Ja (Department of Food and Biotechnology, Hankyong National University) ;
  • Park, Nyun-Ho (Gyeongbuk Institute for Marine Bio-Industry)
  • 권태형 ((재)춘천바이오산업진흥원) ;
  • 이수진 (강원대학교 생약자원학과) ;
  • 박재호 (중원대학교 생약자원학과) ;
  • 김태완 (안동대학교 식품생명공학과) ;
  • 박정자 (한경대학교 식품생명공학과) ;
  • 박년호 ((재)경북해양바이오산업연구원)
  • Received : 2017.03.30
  • Accepted : 2017.05.29
  • Published : 2017.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study aimed to investigate the various biological activities of Geranium thunbergii such as antimicrobial activity and protective effect against oxidative damage. To evaluate its antioxidant and antimicrobial activities, we first performed methanol extraction; this methanol extract was further partitioned using various solvents. And then, its antioxidant activity was measured using various assays including total phenolic content and protection against oxidative DNA damage, and antimicrobial activities were examined using minimum inhibiting concentration (MIC) test, and paper disc method. In addition, high-performance liquid chromatography was performed to analyze the major chemical components of ethyl acetate fraction. The G. thunbergii fraction with ethyl acetate exhibited higher antioxidant and antimicrobial activities than the other fractions. The results showed that G. thunbergii ethyl acetate fraction at $50{\mu}g/mL$ had strong DPPH and ABTS radical scavenging activities of 80.88% and 80.12%, respectively. In addition, the ethyl acetate fraction protected DNA from the oxidative damage induced by ferrous ion and hydroxyl radicals and showed high antimicrobial activity with diameter of inhibition zones ranging from 13.33 to 15.67 mm. High-performance liquid chromatography analysis revealed the major phenolic compounds of G. thunbergii to be ellagic acid and gallic acid. These results suggest that G. thunbergii might protect DNA against oxidative stress induced by reactive oxygen species and can be utilized as a natural source of antioxidant and antimicrobial agent in the food industry.

본 연구에서는 현초의 식품소재 적용과 기능성 소재의 개발을 위해 현초 에틸 아세테이트 분획물을 이용하여 활성산소종의 소거활성과 산화적 DNA 손상 보호효과 및 항균활성에 대해 검증하였다. 현초를 메탄올로 추출하여 얻어진 추출물에 대해 n-hexane, chloroform, ethyl acetate, n-butanol, water의 용매를 이용하여 순차분획을 실시하였고, 얻어진 결과물에 대하여 다양한 항산화 측정 방법을 통하여 항산화 효능을 측정한 결과 에틸 아세테이트 분획물의 경우 DPPH 라디칼 소거능, ABTS 라디칼 소거능 및 환원력에서 효과가 높게 측정 되었으며, $50{\mu}g/mL$의 농도에서 각각 80.88%, 80.12%, 28%를 저해하는 것으로 측정되었다. 이러한 항산화 효능과 함께 산화적 DNA 손상 보호효과를 검증하였고 농도별로 억제하는 경향을 나타냈다. 또한 식품 소재 및 다양한 첨가 소재로 이용하기 위하여 항균활성을 측정하였으며, 에틸 아세테이트 분획물에서 연구에 사용된 모든 균주에 대하여 저해 활성을 보였다. 이러한 활성을 가진 현초 에틸 아세테이트 분획물의 활성물질을 검증하기 위하여 phenolic compound 및 flavonoid 대조군을 이용하여 LC 분석을 하였다. 그 결과 ellagic acid와 gallic aicd가 검출 되었으며 각각 55.14 mg/g, 5.42 mg/g 측정 되었다. 이는 결과적으로 현초는 다양한 식품소재로서의 활용될 수 있으며, 본 논문은 기능성 물질로 활용을 위한 기초자료가 될 것으로 사료 된다.

Keywords

References

  1. Zeng WC, Zhang Z, Gao H, Jia LR, He Q (2012) Chemical composition, antioxidant, and antimicrobial activities of essential oil from pine needle (Cedrus deodara). J Food Sci, 77, 824-829 https://doi.org/10.1111/j.1750-3841.2012.02767.x
  2. Lee YH, Kang YM, Heo MJ, Kim GE, Bhattarai G, Lee NH, Yu MK, Yi HK (2013) The survival role of peroxisome proliferator-activated receptor gamma induces odontoblast differentiation against oxidative stress in human dental pulp cells. J Endod, 39, 236-241 https://doi.org/10.1016/j.joen.2012.11.006
  3. Jeong JB, De Lumen BO, Luman D, Jeong HJ (2010) Lunasin peptide purified from Solanum nigrum L. protects DNA from oxidative damage by suppressing the generation of hydroxyl radical via blocking fenton reaction. Cancer Lett, 293, 58-64 https://doi.org/10.1016/j.canlet.2009.12.019
  4. Jo YH, Seo GU, Yuk HK, Lee SC (2012) Antioxidant and tyrosinase inhibitory activities of methanol extracts from Magnolia denudate and Magnolia denudate var. purpurascens flowers. Food Res Int, 47, 197-200 https://doi.org/10.1016/j.foodres.2011.05.032
  5. Yildirim A, Mavi A, Oktay M, Kara AA, Algur OF, Bilaloglu V (2000) Comparison of antioxidant and antimicrobial activities of tilia (Tilia Argentea Desf Ex DC), sage (Salvia Triloba L.), and black tea (Camellia Sinensis) extracts. J Agric Food Chem, 48, 5030-5034 https://doi.org/10.1021/jf000590k
  6. Zeng LB, Zhang ZR, Luo ZH, Zhu JX (2011) Antioxidant activity and chemical constituents of essential oil and extracts of Rhizoma Homalomenae. Food Chem, 125, 456-463 https://doi.org/10.1016/j.foodchem.2010.09.029
  7. Yoo EA, Kim SD, Lee WM, Park HJ, Kim SK, Cho JY, Min W, Rhee MH (2008) Evaluation of antioxidant, antinociceptive, and anti-inflammatory activities of ethanol extracts from Aloe saponaria Haw. Phytother Res, 22, 1389-1395 https://doi.org/10.1002/ptr.2514
  8. Suh HJ, Kim SR, Lee KS, Park S, Kang SC (2010) Antioxidant activity of various solvent extracts from Allomyrina dichotoma (Arthropoda: Insecta) larvae. J Photochem Photobiol B, 99, 67-73 https://doi.org/10.1016/j.jphotobiol.2010.02.005
  9. Feng S, Zeng W, Luo F, Zhao J, Yang Z, Sun Q (2010) Antibacterial activity of organic acids in aqueous extracts from pine needles (Pinus massoniana Lamb.). Food Sci Biotechnol, 19, 35-41 https://doi.org/10.1007/s10068-010-0005-2
  10. Cote J, Caillet S, Doyon G, Dussault D, Sylvain JF (2011) Lacroix M. Antimicrobial effect of cranberry juice and extracts. Food Control, 22, 1413-1418 https://doi.org/10.1016/j.foodcont.2011.02.024
  11. Khan H, Jan SA, Javed M, Shaheen R, Khan Z, Ahmad A, Safi SZ, Imran M (2016) Nutritional composition, antioxidant and antimicrobial activities of selected wild edible plants. J Food Biochem, 40, 61-70 https://doi.org/10.1111/jfbc.12189
  12. Ismail T, Akhtar S, Sestili P, Riaz M, Isamil A, Labbe RG (2016) Antioxidant, antimicrobial and urease inhibitory activities of phenolics-rich pomegranate peel hydro-alcoholic extracts. J Food Biochem, 40, 550-558 https://doi.org/10.1111/jfbc.12250
  13. Karamac M, Kosinska A, Estrella I, Hernandez T, Duenas M (2012) Antioxidant activity of phenolic compounds identified in sunflower seeds. Eur Food Res Technol, 235, 221-230 https://doi.org/10.1007/s00217-012-1751-6
  14. Zhou J, Li P, Cheng N, Gao H, Wang B, Wei Y, Cao W (2012) Protective effects of buckwheat honey on DNA damage induced by hydroxyl radicals. Food Chem Toxicol, 50, 2766-2773 https://doi.org/10.1016/j.fct.2012.05.046
  15. Lee SH, Kang KM, Park HJ, Baek, LM (2009) Physiological characteristics of medicinal plant extracts for use as functional materials in seasoning sauce for pork meat. Korean J Food Sci Technol, 41, 57-63
  16. Choi SJ, Kim JK, Jang JM, Shin KH, Lim SS (2012) Rapid identification of the ${\alpha}$-glucosidase inhibitory compounds from thunberg's geranium (Geranium thunbergii Sieb. et zucc). Food Sci Biotechnol, 21, 987-996 https://doi.org/10.1007/s10068-012-0129-7
  17. Liu QH, Woo ER (2008) Inhibitory activity of IL-6 production by flavonoids and phenolic compounds from Geranium thunbergii. Nat Prod Sci, 14, 16-20
  18. Kim MB, Park JS, Lim SB (2010) Antioxidant activity and cell toxicity of pressurized liquid extracts from 20 selected plant species in Jeju, Korea. Food Chem, 122, 546-552 https://doi.org/10.1016/j.foodchem.2010.03.007
  19. Folin O, Denis W (1912) On phosphotungsticphosphomolybdic compounds as color reagents. J Biol Chem, 12, 239-243
  20. Blois MS (1958) Antioxidant determinations by the use of a stable free radical. Nature, 181, 1199-1200 https://doi.org/10.1038/1811199a0
  21. Re R, Pellegrini N, Proteggente A, Pannala A, Tang M, Rice-Evans C (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biol Med, 26, 1231-1237 https://doi.org/10.1016/S0891-5849(98)00315-3
  22. Oyaizu M (1986) Studies on products of browning reaction: antioxidant activities of products of browning reaction prepared from glucosamine. Jpn J Nutr, 44, 307-315 https://doi.org/10.5264/eiyogakuzashi.44.307
  23. Jang M, Jeong SW, Cho SK, Ahn KS, Kim BK, Kim JC (2013) Anti-inflammatory effects of 4 medicinal plant extracts in lipopolysaccharide-induced RAW 264.7 cells. Food Sci Biotechnol, 22, 213-220 https://doi.org/10.1007/s10068-013-0069-x
  24. Tsai YL, Chiou SY, Chan KC, Sung JM, Lin SD (2012) Caffeic acid derivatives, total phenols, antioxidant and antimutagenic activities of Echinacea purpurea flower extracts. LWT-Food Sci Technol, 46, 169-176 https://doi.org/10.1016/j.lwt.2011.09.026
  25. Kim IS, Yang M, Lee OH, Kang SN (2011) The antioxidant activity and the bioactive compound content of Stevia rebaudiana water extracts. LWT-Food Sci Technol, 44, 1328-1332 https://doi.org/10.1016/j.lwt.2010.12.003
  26. Oh J, Jo H, Cho AR, Kim SJ, Han J (2013) Antioxidant and antimicrobial activities of various leafy herbal teas. Food Control, 31, 403-409 https://doi.org/10.1016/j.foodcont.2012.10.021
  27. Floegel A, Kim DO, Chung SJ, Koo SI, Chun OK (2011) Comparison of ABTS/DPPH assays to measure antioxidant capacity in popular antioxidant-rich US foods. J Food Compos Anal, 24, 1043-1048 https://doi.org/10.1016/j.jfca.2011.01.008
  28. Lee KH, Park SN (2008) Antioxidative activities and antiaging effects of Geranium nepalense extracts. J Soc Cosmet Scientists Korea, 34, 25-35
  29. Singh P, Vishwakarma SP, Singh RL (2014) Antioxidant, oxidative DNA damage protective and antimicrobial activities of the plant Trigonella foenum-graecum. J Sci Food Agric, 94, 2497-2504 https://doi.org/10.1002/jsfa.6585
  30. Cheng N, Wang Y, Gao H, Yuan J, Feng F, Cao W, Zheng J (2013) Protective effect of extract of Crataegus pinnatifida pollen on DNA damage response to oxidative stress. Food Chem Toxicol, 59, 709-714 https://doi.org/10.1016/j.fct.2013.07.015
  31. Abbas SR, Sabir SM, Ahmad SD, Boligon AA, Athayde ML (2014) Phenolic profile, antioxidant potential and DNA damage protecting activity of sugarcane (Saccharum officinarum). Food Chem, 147, 10-16 https://doi.org/10.1016/j.foodchem.2013.09.113
  32. Shen X, Sun X, Xie Q, Liu H, Zhao Y, Pan Y, Hwang CA, Wu VCH (2014) Antimicrobial effect of blueberry (Vaccinium corymbosum L.) extracts against the growth of Listeria monocytogenes and Salmonella Enteritidis. Food Control, 35, 159-165 https://doi.org/10.1016/j.foodcont.2013.06.040
  33. Xiong L, Yang J, Jiang Y, Lu B, Hu Y, Zhou F, Mao S, Shen C (2014) Phenolic compounds and antioxidant capacities of 10 common edible flowers from China. J Food Sci, 79, 517-525 https://doi.org/10.1111/1750-3841.12404

Cited by

  1. Secondary Metabolites, Dietary Fiber and Conjugated Fatty Acids as Functional Food Ingredients against Overweight and Obesity vol.13, pp.8, 2018, https://doi.org/10.1177/1934578x1801300836