Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Microbial community analysis of commercial nuruk in Korea using pyrosequencing

파이로시퀀싱을 이용한 상업용 전통누룩의 미생물 군집분석

  • Park, Ji-Hee (Department of Culinary Science and Foodservice Management, Sejong University) ;
  • Kim, Song-Gun (Microbial Resource Center/KCTC, Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Yong-Jae (Microbial Resource Center/KCTC, Korea Research Institute of Bioscience and Biotechnology) ;
  • Chung, Chang-Ho (Department of Culinary Science and Foodservice Management, Sejong University)
  • 박지희 (세종대학교 조리외식경영학과) ;
  • 김성건 (미생물 자원센터, 한국생명공학연구원) ;
  • 이용재 (미생물 자원센터, 한국생명공학연구원) ;
  • 정장호 (세종대학교 조리외식경영학과)
  • Received : 2017.09.05
  • Accepted : 2017.10.17
  • Published : 2018.02.28
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Abstract

Microbial communities of four commercial Korean nuruks were analyzed by the 454 pyrosequencing method to correlate different characteristics of rice wine fermentation. The total and average sequencing reads of fungi in the four nuruks were 14,800 and 3,494, respectively. At the phylum level, Ascomycota was dominant in three nuruks, namely, SH, SS, and JJ, while Zygomycota was dominant in SJ. Saccharomycopsis was dominant in nuruks subjected to longer fermentation periods, such as SH and SS. The total and average sequence reads for bacteria were 31,485 and 7,871, respectively. Bacteria belonging to the phylum Firmicutes were dominant in all samples. SH showed several genera of lactic acid bacteria, such as Lactobacillus, Leuconostoc, Pediococcus, and other minor bacteria. Staphylococcus and Bacillus were the dominant bacteria in JJ and SJ, respectively.

과거 전통방식으로 제조되는 4가지 상업용 시판누룩들을 파이로시퀀싱 방법을 이용하여 미생물군집을 확인하였다. 누룩시료의 진균류 총 유효 sequencing read수는 14,800이였으며 후속 quality trimming, denoising와 chimera removal와 같은 작업을 통해 얻은 평균 sequence read의 수는 3,494이였다. 진균류의 phylum 수준에서 SH, JJ, SS 시료에서는 자낭균류가 우세한 것으로 확인되었으나 SJ는 접합균류가 우세하였다. 발효력과 관계가 있는 것으로 알려진 Saccharomycopsis속 효모분포는 제조과정에서 상대적으로 발효시간이 길었던 SH와 SS의 경우에서 더 많았다. 세균류의 파이로시퀀싱결과 유효 sequencing read수는 31,485이였으며 평균 sequence read는 7,871이였다. 세균의 phylum 수준에서는 모든 시료에서 Firmicutes문이 우세한 것으로 분석되었다. SH의 경우 주로 Lactobacillus속, Leuconostoc속, 기타 Pediococcus속과 이를 포함하는 Lactobacillales목과 Leuconostocaceae과 등 젖산균들이 우세균주로 분포하였다. JJ와 SJ의 경우, 각각 Staphylococcus속과 Bacillus속이 우세균으로 나타났다.

Keywords

References

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