Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Functional role of Forskolin and PD166285 in the development of denuded mouse oocytes

  • Cao, Hongguo (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Bian, Yani (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Zhang, Fei (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Tang, Yunshu (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Li, Caixia (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Chen, Jiemei (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Zhang, Xiaorong (College of Animal Science and Technology, Anhui Agricultural University)
  • Received : 2017.06.08
  • Accepted : 2017.09.04
  • Published : 2018.03.01
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Abstract

Objective: cAMP and mature promoting factor (MPF) play critical roles during the maturation of mammalian oocytes. The aim of this study was to produce the offspring from denuded oocytes (DOs) in mice by regulating cAMP and MPF. Methods: In this study, we used DOs at the germinal vesicle (GV) stage in mice and regulated levels of cAMP and MPF in DOs by adding Forskolin and PD166285 during in vitro maturation without follicle stimulating hormone and luteinizing hormone, respectively. Results: Combined use of $50{\mu}M$ Forskolin for 3 h and $2.5{\mu}M$ PD166285 for additional 21 h enhanced the developmental competence of DOs, maturation rate of DOs was $76.71%{\pm}4.11%$, blastocyst rate was $18.33%{\pm}4.44%$ after parthenogenetic activation (PA). The DOs could successfully be fertilized with sperm in vitro, cleavage rate was $17.02%{\pm}5.82%$ and blastocyst rate was $5.65%{\pm}3.10%$. Besides, 2-cell in vitro fertilization embryos from DOs produced 4 normal live offspring (4/34). Conclusion: The results confirmed that the combination of Forskolin and PD166285 can induce DOs to complete meiosis process and produce normal offspring.

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References

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