Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Immune-Enhancing Effect of Nanometric Lactobacillus plantarum nF1 (nLp-nF1) in a Mouse Model of Cyclophosphamide-Induced Immunosuppression

  • Choi, Dae-Woon (Food Biotechnology Program, Korea University of Science and Technology) ;
  • Jung, Sun Young (Food Biotechnology Program, Korea University of Science and Technology) ;
  • Kang, Jisu (Food Biotechnology Program, Korea University of Science and Technology) ;
  • Nam, Young-Do (Food Biotechnology Program, Korea University of Science and Technology) ;
  • Lim, Seong-Il (Division of Strategic Food Research, Korea Food Research Institute) ;
  • Kim, Ki Tae (Biogenics Korea Co. Ltd.) ;
  • Shin, Hee Soon (Food Biotechnology Program, Korea University of Science and Technology)
  • Received : 2017.09.13
  • Accepted : 2017.11.15
  • Published : 2018.02.28
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Abstract

Nanometric Lactobacillus plantarum nF1 (nLp-nF1) is a biogenics consisting of dead L. plantarum cells pretreated with heat and a nanodispersion process. In this study, we investigated the immune-enhancing effects of nLp-nF1 in vivo and in vitro. To evaluate the immunostimulatory effects of nLp-nF1, mice immunosuppressed by cyclophosphamide (CPP) treatment were administered with nLp-nF1. As expected, CPP restricted the immune response of mice, whereas oral administration of nLp-nF1 significantly increased the total IgG in the serum, and cytokine production (interleukin-12 (IL-12) and tumor necrosis factor alpha (TNF-${\alpha}$)) in bone marrow cells. Furthermore, nLp-nF1 enhanced the production of splenic cytokines such as IL-12, TNF-${\alpha}$, and interferon gamma (IFN-${\gamma}$). In vitro, nLp-nF1 stimulated the immune response by enhancing the production of cytokines such as IL-12, TNF-${\alpha}$, and IFN-${\gamma}$. Moreover, nLp-nF1 given a food additive enhanced the immune responses when combined with various food materials in vitro. These results suggest that nLp-nF1 could be used to strengthen the immune system and recover normal immunity in people with a weak immune system, such as children, the elderly, and patients.

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