Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Association of Salivary Microbiota with Dental Caries Incidence with Dentine Involvement after 4 Years

  • Kim, Bong-Soo (Department of Life Science, Hallym University) ;
  • Han, Dong-Hun (Department of Preventive and Social Dentistry, School of Dentistry, Seoul National University) ;
  • Lee, Ho (Department of Oral and Maxillofacial Surgery, Section of Dentistry, SMG- SNU Boramae Medical Center) ;
  • Oh, Bumjo (Department of Family Medicine, SMG-SNU Boramae Medical Center)
  • Received : 2017.10.20
  • Accepted : 2017.12.21
  • Published : 2018.03.28
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Abstract

Salivary microbiota alterations can correlate with dental caries development in children, and mechanisms mediating this association need to be studied in further detail. Our study explored salivary microbiota shifts in children and their association with the incidence of dental caries with dentine involvement. Salivary samples were collected from children with caries and their subsequently matched caries-free controls before and after caries development. The microbiota was analyzed by 16S rRNA gene-based high-throughput sequencing. The salivary microbiota was more diverse in caries-free subjects than in those with dental caries with dentine involvement (DC). Although both groups exhibited similar shifts in microbiota composition, an association with caries was found by function prediction. Analysis of potential microbiome functions revealed that Granulicatella, Streptococcus, Bulleidia, and Staphylococcus in the DC group could be associated with the bacterial invasion of epithelial cells, phosphotransferase system, and ${\text\tiny{D}}-alanine$ metabolism, whereas Neisseria, Lautropia, and Leptotrichia in caries-free subjects could be associated with bacterial motility protein genes, linoleic acid metabolism, and flavonoid biosynthesis, suggesting that functional differences in the salivary microbiota may be associated with caries formation. These results expand the current understanding of the functional significance of the salivary microbiome in caries development, and may facilitate the identification of novel biomarkers and treatment targets.

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References

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