Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Properties of Tetragenococcus halophilus Strains Isolated from Myeolchi (anchovy)-jeotgal

  • Kim, Jeong A (Division of Applied Life Science (BK21 plus), Graduate School, Gyeongsang National University) ;
  • Yao, Zhuang (Division of Applied Life Science (BK21 plus), Graduate School, Gyeongsang National University) ;
  • Perumal, Venkatesh (Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Kim, Hyun-Jin (Division of Applied Life Science (BK21 plus), Graduate School, Gyeongsang National University) ;
  • Kim, Jeong Hwan (Division of Applied Life Science (BK21 plus), Graduate School, Gyeongsang National University)
  • Received : 2018.04.26
  • Accepted : 2018.08.01
  • Published : 2018.12.28
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Abstract

Halophilic lactic acid bacteria (LAB) were isolated from myeolchi-jeotgal (23% NaCl, w/v) fermented in jangdok (Korean earthenware) located outside a house. They were identified as Tetragenococcus halophilus by 16S rRNA and recA gene sequencing. Four T. halophilus isolates showing high protease activities were selected for further studies. Four strains grew well, reaching $OD_{600}$ values of 0.75-0.92 at 18% NaCl content (w/v) and 0.28-0.44 at 23% salt. They showed rapid growth, attaining $OD_{600}$ values of 1.1-1.2 at $20-30^{\circ}C$, but did not grow at $4^{\circ}C$. At $15^{\circ}C$, the highest $OD_{600}$ values, which exceeded 0.6, were observed at 20 days, and were higher than those of cultures at $37^{\circ}C$ and $42^{\circ}C$ (approximately 0.5). Four isolates grew best in broth where the initial pH was adjusted to 8 and did not grow at $pH{\leq}4$. T. halophilus BS2-36 showed the highest survival ratio of 18.7% after 2 hours of exposure at pH 3. BS2-36 showed the highest survival ratio (1.29%) in presence of 0.3% bile salts. T. halophilus BS2-36 seems a promising candidate as a starter for jeotgal and other fermented foods with high salinities.

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References

  1. Jeong DW, Kim HR, Jung G, Han S, Kim CT, Lee JH. 2014. Bacterial community migration in the ripening of Doenjang, a traditional Korean fermented soybean food. J. Microbiol. Biotechnol. 24: 648-660. https://doi.org/10.4014/jmb.1401.01009
  2. Kim TW, Lee JH, Kim SE, Park MH, Chang HC, Kim HY. 2009. Analysis of microbial communities in doenjang, a Korean fermented soybean paste, using nested PCR-denaturing gradient gel electrophoresis. Int. J. Food Microbiol. 131: 265-271. https://doi.org/10.1016/j.ijfoodmicro.2009.03.001
  3. Fukui Y, Yoshida M, Shozen KI, Funatsu Y, Takano T, Oikawa H, et al. 2012. Bacterial communities in fish sauce mash using culture-dependent and -independent methods. J. Gen. Appl. Microbiol. 58: 273-281. https://doi.org/10.2323/jgam.58.273
  4. Udomsil N, Rodtong S, Tanasupawat S, Yongsawatdigul J. 2010. Proteinase-producing halophilic lactic acid bacteria isolated from fish sauce fermentation and their ability to produce volatile compounds. Int. J. Food Microbiol. 141: 186-194. https://doi.org/10.1016/j.ijfoodmicro.2010.05.016
  5. Han KI, Kim YH, Hwang SG, Jung EG, Patnaik BB, Han YS, et al. 2014. Bacterial community dynamics of salted and fermented shrimp based on denaturing gradient gel electrophoresis. J. Food Sci. 79: M2516-2522. https://doi.org/10.1111/1750-3841.12707
  6. Kim MS, Park EJ. 2014. Bacterial communities of traditional salted and fermented seafoods from Jeju island of Korea using 16S rRNA gene clone library analysis. J. Food Sci. 79: M927-934. https://doi.org/10.1111/1750-3841.12431
  7. Masuda S, Yamaguchi H, Kurokawa T, Shirakami T, Tsuji RF, Nishimura I. 2008. Immunomodulatory effect of halophilic lactic acid bacterium Tetragenococcus halophilus Th221 from soy sauce moromi grown in high-salt medium. Int. J. Food Microbiol. 121: 245-252. https://doi.org/10.1016/j.ijfoodmicro.2007.10.011
  8. Tanaka Y, Watanabe J, Mogi Y. 2012. Monitoring of the microbial communities involved in the soy sauce manufacturing process by PCR-denaturing gradient gel electrophoresis. Food Microbiol. 31: 100-106. https://doi.org/10.1016/j.fm.2012.02.005
  9. Lee M, Kim MK, Vancanneyt M, Swings J, Kim SH, Kang MS, et al. 2005. Tetragenococcus koreensis sp. nov., a novel rhamnolipidproducing bacterium. Int. J. Syst. Evol. Microbiol. 55: 1409-1413. https://doi.org/10.1099/ijs.0.63448-0
  10. Amadoro C, Rossi F, Piccirilli M, Colavita G. 2015. Tetragenococcus koreensis is part of the microbiota in a traditional Italian raw fermented sausage. Food Microbiol. 50: 78-82. https://doi.org/10.1016/j.fm.2015.03.011
  11. Juste A, Van Trappen S, Verreth C, Cleenwerck I, De Vos P, Lievens B, et al. 2012. Characterization of Tetragenococcus strains from sugar thick juice reveals a novel species, Tetragenococcus osmophilus sp. nov., and divides Tetragenococcus halophilus into two subspecies, T. halophilus subsp. halophilus subsp. nov. and T. halophilus subsp. flandriensis subsp. nov. Int. J. Syst. Evol. Microbiol. 62: 129-137. https://doi.org/10.1099/ijs.0.029157-0
  12. Udomsil N, Rodtong S, Choi YJ, Hua Y, Yongsawatdigul J. 2011. Use of Tetragenococcus halophilus as a starter culture for flavor improvement in fish sauce fermentation. J. Agric. Food Chem. 59: 8401-8408. https://doi.org/10.1021/jf201953v
  13. Harada R, Yuzuki M, Ito K, Shiga K, Bamba T, Fukusaki E. 2018. Microbe participation in aroma production during soy sauce fermentation. J. Biosci. Bioeng. 125: 688-694. https://doi.org/10.1016/j.jbiosc.2017.12.004
  14. Jeong DW, Heo S, Lee JH. 2017. Safety assessment of Tetragenococcus halophilus isolates from doenjang, a Korean high-salt-fermented soybean paste. Food Microbiol. 62: 92-98. https://doi.org/10.1016/j.fm.2016.10.012
  15. Jeong SJ, Heo K, Park JY, Lee KW, Park JY, Joo SH, et al. 2015. Characterization of AprE176, a fibrinolytic enzyme from Bacillus subtilis HK176. J. Microbiol. Biotechnol. 25: 89-97. https://doi.org/10.4014/jmb.1409.09087
  16. Jeong SJ, Park JY, Lee JY, Lee KW, Cho KM, Kim GM, et al. 2015. Improvement of fibrinolytic activity of Bacillus subtilis 168 by integration of a fibrinolytic gene into the chromosome. J. Microbiol. Biotechnol. 25: 1863-1870. https://doi.org/10.4014/jmb.1505.05062
  17. Uchida M, Miyoshi T, Yoshida G, Niwa K, Mori M, Wakabayashi H. 2014. Isolation and characterization of halophilic lactic acid bacteria acting as a starter culture for sauce fermentation of the red alga Nori (Porphyra yezoensis). J. Appl. Microbiol. 116: 1506-1520. https://doi.org/10.1111/jam.12466
  18. Tham CSC, Peh KK, Bhat R, Liong MT. 2012. Probiotic properties of bifidobacteria and lactobacilli isolated from local dairy products. Ann. Microbiol. 62: 1079-1087. https://doi.org/10.1007/s13213-011-0349-8
  19. Lee KW, Park JY, Jeong HR, Heo HJ, Han NS, Kim JH. 2012. Probiotic properties of Weissella strains isolated from human feces. Anaerobe 18: 96-102. https://doi.org/10.1016/j.anaerobe.2011.12.015
  20. Lee ME, Jang JY, Lee JH, Park HW, Choi HJ, Kim TW. 2015. Starter cultures for kimchi fermentation. J. Microbiol. Biotechnol. 25: 559-568. https://doi.org/10.4014/jmb.1501.01019