Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Draft genome sequence of a bacterial plant pathogen Erwinia pyrifoliae strain EpK1/15 isolated from an apple twig showing black shoot blight

가지검은마름병 병징을 보이는 사과나무 가지에서 분리한 식물병원세균인 Erwinia pyrifoliae EpK1/15 균주의 유전체 해독

  • Lee, Gyu Min (Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University) ;
  • Oh, Eom-Ji (Department of Horticultural Biotechnology, College of Life Sciences, Kyung Hee University) ;
  • Ko, Seyoung (Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University) ;
  • Park, Jungkum (Department of Horticultural Biotechnology, College of Life Sciences, Kyung Hee University) ;
  • Park, Duck Hwan (Applied Biology Program, Division of Bioresource Sciences, Kangwon National University) ;
  • Kim, Donghyuk (Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University) ;
  • Oh, Chang-Sik (Department of Horticultural Biotechnology, College of Life Sciences, Kyung Hee University)
  • 이규민 (경희대학교 생명공학원) ;
  • 오엄지 (경희대학교 원예생명공학과) ;
  • 고세영 (경희대학교 생명공학원) ;
  • 박정금 (경희대학교 원예생명공학과) ;
  • 박덕환 (강원대학교 응용생물학과) ;
  • 김동혁 (경희대학교 생명공학원) ;
  • 오창식 (경희대학교 원예생명공학과)
  • Received : 2017.11.07
  • Accepted : 2017.12.29
  • Published : 2018.03.31
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Abstract

Erwinia pyrifoliae is a Gram-negative bacterium causing black shoot blight in apple and Asian pear trees. E. pyrifoliae strain EpK1/15 was isolated in 2014 from an apple twig from the Pocheon, Gyeonggi-do, South Korea. In this study, we report the draft genome sequence of E. pyrifoliae EpK1/15 using PacBio RS II platform. The draft genome is comprised of a circular chromosome with 4,027,225 bp and 53.4% G + C content and a plasmid with 48,456 bp and 50.3% G + C content. The draft genome includes 3,798 protein-coding genes, 22 rRNA genes, 77 tRNA genes, 13 non-coding RNA genes, and 231 pseudo genes.

Erwinia pyrifoliae는 그람 음성 세균으로 사과와 배에 가지검은마름병을 일으킨다. E. pyrifoliae EpK1/15 균주가 병징을 보이는 경기도 포천지역의 사과나무 가지에서 2014년도에 분리되었다. 본 논문에서는 PacBio RS II 플랫폼을 이용하여 E. pyrifoliae EpK1/15 균주의 전체 유전체를 분석하여 보고한다. 본 균주는 G + C 비율이 53.4%이며, 4,027,225 bp로 구성된 염색체와 G + C 비율이 50.3%이며, 48,456 bp로 구성된 plasmid를 지니고 있다. 이들 염색체와 plasmid DNA에서 3,798개의 단백질 코딩 유전자, 22개의 rRNA, 77개의 tRNA, 13개의 non-coding RNA 및 231개의 위유전자(pseudo gene)가 확인되었다.

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References

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