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Draft genome sequence of lytic bacteriophage SA7 infecting Staphylococcus aureus isolates

Staphylococcus aureus 분리주를 감염시키는 용균 박테리오파지 SA7의 유전체 염기서열 초안

  • Kim, Youngju (Optipharm Inc.) ;
  • Lee, Gyu Min (Department of Genetic Engineering and Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University) ;
  • Taizhanova, Assiya (Department of Genetic Engineering and Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University) ;
  • Han, Beom Ku (Optipharm Inc.) ;
  • Kim, Hyunil (Optipharm Inc.) ;
  • Ahn, Jeong Keun (Department of Microbiology & Molecular Biology, Chungnam National University) ;
  • Kim, Donghyuk (Department of Genetic Engineering and Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University)
  • Received : 2017.12.19
  • Accepted : 2018.01.02
  • Published : 2018.03.31

Abstract

Staphylococcus aureus is a Gram-positive and a round-shaped bacterium of Firmicutes phylum, and is a common cause of skin infections, respiratory infections, and food poisoning. Bacteriophages infecting S. aureus can be an effective treatment for S. aureus infections. Here, the draft genomic sequence is announced for a lytic bacteriophage SA7 infecting S. aureus isolates. The bacteriophage SA7 was isolated from a sewage water sample near a livestock farm in Chungcheongnam-do, South Korea. SA7 has a genome of 34,730 bp and 34.1% G + C content. The genome has 53 protein-coding genes, 23 of which have predicted functions from BLASTp analysis, leaving the others conserved proteins with unknown function.

포도상구균(Staphylococcus aureus)은 그람양성이고 구형의 박테리아로 Firmicutes 문에 속하며, 피부나 호흡기 감염 그리고 식중독의 주요 감염원 중에 하나이다. 포도상구균을 감염시키는 박테리오파지는 포도상구균 감염에 효과적인 처방으로 쓰일 수 있다. 본 연구에서는 충청남도에 위치한 가축 농장의 오수에서 분리된 포도상구균 박테리오파지 SA7 균주의 유전체 초안을 분석하였다. 본 균주는 G + C 비율이 34.1%이며, 34,730 bp 로 구성된 dsDNA 를 지니고 있었다. 염색체에서 53 개의 단백질 코딩 유전자가 확인되었으며, 이 중 23 개의 유전자는 BLASTp 분석으로부터 기능을 가지고 있다고 추정되었다. 나머지는 가설 단백질 혹은 보존 단백질이었다.

Keywords

References

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