Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Draft genome sequence of lytic bacteriophage CF1 infecting Citrobacter freundii isolates

Citrobacter freundii 분리주를 감염시키는 용균 박테리오파지 CF1의 유전체 염기서열 초안

  • Kim, Youngju (Optipharm Inc.) ;
  • Ko, Seyoung (Department of Genetic Engineering and Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University) ;
  • Yeon, Young Eun (Optipharm Inc.) ;
  • Lim, Jaewon (Department of Genetic Engineering and Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University) ;
  • Han, Beom Ku (Optipharm Inc.) ;
  • Kim, Hyunil (Optipharm Inc.) ;
  • Ahn, Jeong Keun (Department of Microbiology & Molecular Biology, Chungnam National University) ;
  • Kim, Donghyuk (Department of Genetic Engineering and Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University)
  • Received : 2017.12.22
  • Accepted : 2018.01.08
  • Published : 2018.03.31
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Abstract

Citrobacter freundii is a facultative anaerobic and a Gram-negative bacterium of Enterobacteriaceae family, and is an opportunistic pathogen. Bacteriophages infecting C. freundii can be an effective treatment for C. freundii infections. Here, the complete genomic sequence is announced for a lytic bacteriophage CF1 infecting C. freundii isolates.

본 연구에서는 돼지 축사 근처 하수 오물에서 분리된 그람 음성균이자 항생제 내성을 쉽게 획득하여 병원성을 띄는 균주인 Citrobacter freundii를 host로 하는 박테리오파지의 유전체 분석을 수행하였다. 본 박테리오파지는 G + C 비율이 42.65%이며, 50,339 bp로 구성된 유전체 DNA를 지니고 있었다. 이러한 유전체 DNA에서 89개의 단백질 코딩 유전자가 확인 되었으며, 이 중 55개의 유전자는 BLASTP 분석으로부터 기능을 가지고 있다고 추정되었다. 또한 RNA는 확인되지 않았다.

Keywords

References

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