Quantitative Determination of the Bioactive Marker Components in Gyeji-tang Using LC-ESI-MS/MS

LC-ESI-MS/MS를 이용한 계지탕 중 주요 성분 분석

  • Seo, Chang-Seob (K-herb Research Center, Korea Institute of Oriental Medicine) ;
  • Ha, Hyekyung (K-herb Research Center, Korea Institute of Oriental Medicine)
  • 서창섭 (한국한의학연구원 K-herb연구단) ;
  • 하혜경 (한국한의학연구원 K-herb연구단)
  • Received : 2017.11.27
  • Accepted : 2018.02.12
  • Published : 2018.03.31

Abstract

A traditional herbal formula, Gyeji-tang has been used to treat the early colds, headache, chills, and fever in Asian countries. In this study, we were performed simultaneous determination of the 14 bioactive marker compounds, gallic acid, spinosin, paeoniflorin, albiflorin, liquiritin apioside, liquiritin, 6'''-feruloylspinosin, liquilitigenin, coumarin, cinnmamic acid, benzoylpaeoniflorin, cinnamaldehyde, glycyrrhizin, and 6-gingerol in Gyeji-tang using an ultra-performance liquid chromatography-electrospray ionization-mass spectrometry (UPLC-ESI-MS/MS). Analytical column was used a Waters Acquity UPLC BEH $C_{18}$ analytical column ($2.1{\times}100mm$, $1.7{\mu}m$) and maintained at $45^{\circ}C$ with a flow rate of 0.3 mL/min. The mobile phase consists of 0.1% (v/v) formic acid in water and acetonitrile with gradient elution. The MS analysis was conducted using multiple reaction monitoring in the positive and negative modes by a Waters ACQUITY TQD LC-MS/MS system. The calibration curves of 14 bioactive marker compounds showed linearity with correlation coefficients ${\geq}0.9798$. The limits of detection and quantification values were in the range of 0.11-6.66 ng/mL and 0.34-19.99 ng/mL, respectively. As a result of the analysis using the established LC-MS/MS method, the amounts of tested 14 compounds in the lyophilized Gyeji-tang sample were detected up to $85.7{\mu}g/g$. These results may be useful for quality assessment of a traditional herbal formulas.

Acknowledgement

Grant : 한약 처방의 과학적 근거 기반 구축사업

Supported by : 한국한의학연구원

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