Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Antioxidant effect and inhibitory activities of ethyl acetate fraction from Gardenia jasminoides extract on nitric oxide production and pancreatic cancer cell proliferation

치자 에틸아세테이트 분획의 산화방지, 산화질소 제거 및 암세포증식 억제 활성

  • Park, Min Kyung (Department of Natural Medicine Resources, Semyung University) ;
  • Yoon, Hye Ji (Department of Natural Medicine Resources, Semyung University) ;
  • Lee, Hwa Jin (Department of Natural Medicine Resources, Semyung University)
  • 박민경 (세명대학교 자연약재과학과) ;
  • 윤혜지 (세명대학교 자연약재과학과) ;
  • 이화진 (세명대학교 자연약재과학과)
  • Received : 2017.11.16
  • Accepted : 2018.01.11
  • Published : 2018.04.30
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Abstract

To evaluate the radical scavenging activity of phenolic-rich fractions of Gardenia jasminoides, we first measured the levels of total polyphenols in hexane, ethyl acetate, and butanol fractions from the extract of G. jasminoides. The ethyl acetate fraction of G. jasminoides extract (GJ-EA) showed high level of phenolics, potent reducing power, and 2,2-diphenyl-1-picrylhydrazyl/2,2'-azino-bis 3-ethylbenzothiazoline-6-sulfonic acid radical scavenging effect. In addition, GJ-EA inhibited the overproduction of nitric oxide in lipopolysaccharide-activated BV-2 microglia. Furthermore, we found that GJ-EA suppressed $H_2O_2$-induced PANC-1 pancreatic cancer cell proliferation in a concentration-dependent manner and also reduced their migratory ability. These results suggest that GJ-EA may be a good source for functional foods with antioxidant and chemo-preventive activities.

치자 추출물의 극성에 따른 분획-헥세인, 에틸아세테이트와 뷰탄올 분획의 폴리페놀 함량을 평가한 결과, 에틸아세테이트 분획이 다량의 폴리페놀을 함유함을 하였고, DPPH와 ABTS 라디칼 제거와 환원력 실험에서 강력한 라디칼 제거와 환원능력을 나타냈다. 또한 치차 에틸아세테이트 분획(GJ-EA)의 라디칼 제거 활성을 세포수준에서 평가한 결과, LPS로 활성화된 BV-2 미세아교세포에서 발생하는 과량의 산화질소 생성을 억제하였고, 과산화수소를 처리한 췌장암 세포주의 증식과 이동성에 대해 억제효과를 나타내었다. 따라서 치자 에틸아세테이트 분획(GJ-EA)이 라디칼 제거효과, 과산화수소 유도형 암세포의 증식과 이동성에 대한 억제효과를 가진다는 것을 확인하여 치자 에틸아세테이트 분획(GJ-EA)을 이용한 기능성 식품소재 개발을 위한 기초자료로 활용될 수 있음을 제시하였다.

Keywords

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