Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Study on the antioxidant, anti-inflammatory and Preservative features Water Chestnut

능실의 항산화, 항염증 및 방부 효과 연구

  • Jang, Hye In (School of Cosmetic Science and Beauty Biotechnology, Semyung University)
  • 장혜인 (세명대학교 화장품뷰티생명공학부)
  • Received : 2018.07.10
  • Accepted : 2018.09.18
  • Published : 2018.09.30
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Abstract

The purpose of this study was to assess the antioxidant, anti-inflammatory and preservative effects of Water Chestnut from 70% ethanol extracts. The toxicity of extracts from Water Chestnut investigated using the RAW 264.7 cell showed higher than 90% of cell survival rate. The total content of polyphenol ethanol extract was $353.1{\pm}5.6mg/g$, while the total content of flavonoid was $26.2{\pm}1.4mg/g$. With a concentration level of $1{\sim}1000 ({\mu}g/m{\ell})$ ethanol extract of Water Chestnut the range of removal of 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) radicals was $17.0{\pm}2.8%{\sim}88.6{\pm}0.6%$ respectively and the range of removal of 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radicals was also $2.3{\pm}0.8%{\sim}93.9{\pm}0.2%$ respectively. There were decreases in reactive oxygen species(ROS) generations ethanol extracts of Water Chestnut 1, 10, $100({\mu}g/m{\ell})$ and significance decrease at $100{\mu}g/m{\ell}$ (p<.01). As a result of measuring the Nitric Oxide(NO) generation amount of Water Chestnut extract 1, 10, $100({\mu}g/m{\ell})$ concentration exhibited significant (p<.001, p<.01) decreases. For the anti-bacterial features using a paper disk and the preservative features using petri film, no significance was found and therefore water chestnut extracts had not anti-bacterial or preservative features.

본 연구는 70 % 에탄올 추출물에서 추출한 Water Chestnut의 항산화, 항 염증 및 방부 효과를 평가하고자 하였다. RAW 264.7 세포를 사용하여 조사한 마름열매 추출물의 독성은 세포 생존율의 90 % 이상을 나타내었으며 폴리페놀 추출물의 총 함량은 $353.1{\pm}5.6mg/g$이었고 플라보노이드 총 함량은 $26.2{\pm}1.4mg/g$이었다. 항산화 활성을 확인하기 위한 1, 1-diphenyl-2-picryl-hydrazyl(DPPH) 라디컬 감소는 마름 열매 추출물 농도 $1{\sim}1000({\mu}g/m{\ell})$범위에서 각각 $17.0{\pm}2.8%{\sim}88.6{\pm}0.6%$의 감소효과를 보였고 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid(ABTS) 라디칼의 제거 범위는 각각 $2.3{\pm}0.8%{\sim}93.9{\pm}0.2%$ 였다. 활성 산소종 (ROS)에서의 효과는 1, 10, $100({\mu}g/m{\ell})$, $100{\mu}g/m{\ell}$ 농도에서 p <.01의 유의 한 감소를 보였다. 1, 10, $100({\mu}g/m{\ell})$ 농도의 Nitric Oxide (NO) 생성량을 측정 한 결과, 유의 한 (p <.001, p <.01)의 감소가 나타났다. 미생물에의 방부효과를 확인하기 위한 페이퍼 디스크를 사용하는 항 박테리아 기능과 페트리 필름을 사용하는 방부제 특성에 대해서는 유의한 효과가 나타나지 않았다.

Keywords

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