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Development and Validation of the Simultaneous Analytical Method of Urinary Metals and Metalloids for the National Biomonitoring Programs

국가 바이오모니터링 프로그램을 위한 소변 중 금속류 동시분석법 개발 및 검증

  • Cho, Yong Min (Institute for Life & Environmental Technology, Smartive Corporation) ;
  • Yang, Minho (Institute for Life & Environmental Technology, Smartive Corporation) ;
  • Im, Hosub (Institute for Life & Environmental Technology, Smartive Corporation) ;
  • Cha, Sangwon (Department of Chemistry, Hankuk University of Foreign Studies) ;
  • Lee, Jaeick (Doping Control Center, Korea Institute of Science and Technology) ;
  • Kim, Ki Hun (Doping Control Center, Korea Institute of Science and Technology) ;
  • Han, Sang Beom (Department of Pharmaceutical Analysis, College of Pharmacy, Chung-Ang University)
  • 조용민 (주식회사 스마티브 생명환경연구소) ;
  • 양민호 (주식회사 스마티브 생명환경연구소) ;
  • 임호섭 (주식회사 스마티브 생명환경연구소) ;
  • 차상원 (한국외국어대학교 화학과) ;
  • 이재익 (한국과학기술연구원 도핑콘트롤센터) ;
  • 김기훈 (한국과학기술연구원 도핑콘트롤센터) ;
  • 한상범 (중앙대학교 약학대학)
  • Received : 2019.09.17
  • Accepted : 2019.11.06
  • Published : 2019.12.31

Abstract

Objectives: This study developed and validated an analysis method of urinary metals and metalloids that can be applied inductively with coupled plasma mass spectrometry (ICP-MS). Methods: 0.3 mL of urine was used to analyze 25 metal and metalloid compounds using ICP-MS. The validation of the analytical method included linearity, accuracy, precision, and the calculation of detection limits. In addition, a comparison test was performed with the graphite furnace atomic absorption spectrometry (GF-AAS) method, which is the current standard method, with urine samples of 66 healthy subjects. Results: The linearities (R2) of calibration curves of all 25 compounds were ≥ 0.999. Of the 25 compounds, the intra-day and inter-day accuracy% of 17 and 20 met ≤15%, respectively. In addition, fifteen compounds showed ≤15% recovery% for certificated reference materials. Intraclass correlation coefficients of the comparison between the current methods and new methods in this study were 0.952 (p-value<0.001) and 0.911 (p-value<0.001) for urinary cadmium and mercury, respectively. Conclusion: This study proposes an efficient simultaneous methodology that can analyze multi elements in smaller sample amounts. More reproduction experiments are needed in the future.

Keywords

Acknowledgement

Supported by : 환경부

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