Journal of Sensor Science and Technology (센서학회지)

The Korean Sensors Society (한국센서학회)
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Immunoaffinity Characteristics of Exosomes from Breast Cancer Cells Using Surface Plasmon Resonance Spectroscopy

  • Sohn, Young-Soo (Department of Biomedical Engineering, Daegu Catholic University) ;
  • Na, Wonhwi (Nano Biofluignostics Research Center, Korea University) ;
  • Jang, Dae-Ho (Nano Biofluignostics Research Center, Korea University)
  • Received : 2019.10.24
  • Accepted : 2019.11.28
  • Published : 2019.11.30
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Abstract

Exosomes, known as nanoscale extracellular vesicles in the range of 30-150 nm, are known to contain clinically significant information. However, there is still insufficient information on exosomal membrane proteins for cancer diagnosis. In this work, we investigated the characteristics of the membrane proteins of exosomes shed by cultured breast cancer cell lines using a surface plasmon resonance (SPR) spectroscopy and pre-activated alkanethiols modified sensor chips. The antibodies of breast cancer biomarkers such as MCU-16, EpCAM, CD24, ErbB2, and CA19-9 were immobilized on the pre-activated alkanethiols surfaces without any activation steps. The purified exosomes were loaded onto each antibody surface. The affinity rank of the antibody surfaces was decided by the relative capture efficiency factors for the exosomes. In addition, an antibody with a relative capture efficiency close to 100% was tested with exosome concentration levels of 104/µl, 105/µl, and 106/µl for quantitative analysis.

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References

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