Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Improving Pneumovirus Isolation Using a Centrifugation and AZD1480 Combined Method

  • Lee, Hansaem (Center for Infectious Diseases Research, National Institute of Health, Korea Centers for Diseases Control and Prevention) ;
  • Woo, Hye-Min (Center for Infectious Diseases Research, National Institute of Health, Korea Centers for Diseases Control and Prevention) ;
  • Kim, Kisoon (Center for Infectious Diseases Research, National Institute of Health, Korea Centers for Diseases Control and Prevention) ;
  • Park, Sehee (Department of Microbiology, College of Medicine, Korea University) ;
  • Park, Man-Seong (Department of Microbiology, College of Medicine, Korea University) ;
  • Kim, Sung Soon (Center for Infectious Diseases Research, National Institute of Health, Korea Centers for Diseases Control and Prevention) ;
  • Kim, You-Jin (Center for Infectious Diseases Research, National Institute of Health, Korea Centers for Diseases Control and Prevention)
  • Received : 2019.06.29
  • Accepted : 2019.09.24
  • Published : 2019.12.28
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Abstract

The isolation of respiratory viruses, especially from clinical specimens, often shows poor efficiency with classical cell culture methods. The lack of suitable methods to generate virus particles inhibits the development of diagnostic assays, treatments, and vaccines. We compared three inoculation methods, classical cell culture, the addition of a JAK2 inhibitor AZD1480, and centrifugation-enhanced inoculation (CEI), to replicate human respiratory syncytial virus (HRSV) and human metapneumovirus (HMPV). In addition, a combined method using AZD1480 treatment and CEI was used on throat swabs to verify that this method could increase virus isolation efficiency from human clinical specimens. Both CEI and AZD1480 treatment increased HRSV and HMPV genome replication. Also, the combined method using CEI and AZD1480 treatment enhanced virus proliferation synergistically. The combined method is particularly suited for the isolation of interferon-sensitive or slowly growing viruses from human clinical specimens.

Keywords

References

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