DOI QR코드

DOI QR Code

Antioxidant contents and activities of twelve varieties of vegetable sprouts

  • Park, Hyunjeong (Department of Food Engineering, Dankook University) ;
  • Shin, Youngjae (Department of Food Engineering, Dankook University) ;
  • Kim, Young-Jun (Department of Food Science and Technology, Seoul National University of Science and Technology)
  • Received : 2019.05.13
  • Accepted : 2019.05.24
  • Published : 2019.06.30

Abstract

This study was conducted to investigate the antioxidant contents and activities of twelve vegetable sprouts (broccoli, red radish, radish, mizuna, kale, taatsai, pak choi, Chinese cabbage, turnip, rapeseed, chicory, and alfalfa). The total flavonoid contents of the broccoli, red radish, and radish sprout were $25.36{\pm}0.13$, $25.26{\pm}1.80$, and $25.16{\pm}1.25mg$ CE/100 g FW, respectively, and were significantly higher than those of the other tested vegetables. Radish sprouts had the highest total phenolic content (112.42 mg GAE/100 g FW), followed by red radish and broccoli sprouts. The main polyphenols in the vegetable sprouts were epicatechin and chlorogenic acid, but they varied across sprout varieties. The correlation between total flavonoids and total phenolics for the 12 vegetable sprouts was very high (r=0.926). The total antioxidant activity (DPPH and ABTS radical scavenging activities) was also highly correlated with total flavonoids and total phenolics.

Keywords

SPGHB5_2019_v51n3_207_f0001.png 이미지

Fig. 1. Total flavonoids content of vegetable sprouts. Error bars represent standard deviation and the different letter above the bar indicates significant differences based on Duncan's multiple range test (p<0.05).

SPGHB5_2019_v51n3_207_f0002.png 이미지

Fig. 2. Total phenolics content of vegetable sprouts. Error bars represent standard deviation and the different letter above the bar indicates significant differences based on Duncan's multiple range test (p<0.05).

SPGHB5_2019_v51n3_207_f0003.png 이미지

Fig. 3. Polyphenol contents of vegetable sprouts (A; Chlorogenic acid, B; Epicatechin). Error bars represent standard deviation and the different letter above the bar indicates significant differences based on Duncan's multiple range test (p<0.05).

SPGHB5_2019_v51n3_207_f0004.png 이미지

Fig. 4. DPPH free radical scavenging activities of vegetable sprouts. Error bars represent standard deviation and the different letter above the bar indicates significant differences based on Duncan's multiple range test (p<0.05).

SPGHB5_2019_v51n3_207_f0005.png 이미지

Fig. 5. ABTS free radical scavenging activities of vegetable sprouts. Error bars represent standard deviation and the different letter above the bar indicates significant differences based on Duncan's multiple range test (p<0.05).

Table 1. Correlation of twelve varieties of vegetable sprouts between antioxidant compounds and activities

SPGHB5_2019_v51n3_207_t0001.png 이미지

References

  1. Alvares-Jubete L, Wijngaard H, Arendt EK, Gallagher E. Polyphenol composition and in vitro antioxidant activity of amaranth, quinoa, buckwheat and wheat as affected by sprouting and baking. Food Chem. 119: 770-778 (2010) https://doi.org/10.1016/j.foodchem.2009.07.032
  2. Brand-Willams W, Cuvelier ME, Berset C. Use of free radical method to evaluate antioxidant activity. Lebensm. Wiss. Technol. 28: 25-30 (1995) https://doi.org/10.1016/S0023-6438(95)80008-5
  3. Chen H, Zuo Y, Deng Y. Separation and determination of flavonoids and other phenolic compounds in cranberry juice by high-performance liquid chromatography. J. Chromatogr. A. 913: 387-395 (2001) https://doi.org/10.1016/S0021-9673(00)01030-X
  4. Chon SU, Kim DK, Kim YM. Phenolics content and antioxidant activity of sprouts in several legume crops. Korean J. Plant Res. 26: 159-168 (2013) https://doi.org/10.7732/kjpr.2013.26.2.159
  5. Goyeneche R, Roura S, Ponce A, Vega-Galvez A, Quispe-Fuentes I, Uribe E, Scala KD. Chemical characterization and antioxidant capacity of red radish (Raphanus sativus L.) leaves and roots. J. Funct. Foods 16: 256-264 (2015) https://doi.org/10.1016/j.jff.2015.04.049
  6. Han JH, Moon HK, Chung SK, Kang WW. Comparison of antioxidant activities of radish bud (Raphanus sativus L.) according to extraction solvents and sprouting period. J. Korean Soc. Food Sci. Nutr. 42: 1767-1775 (2013) https://doi.org/10.3746/jkfn.2013.42.11.1767
  7. Jun SY, Kim TH, Hwang SH. The consumption status and preference for sprouts and leafy vegetables. Korean J. Food Preserv. 19: 783-791 (2012) https://doi.org/10.11002/kjfp.2012.19.5.783
  8. Kim EJ, Choi JY, Yu MR, Kim YM, Lee SH, Lee BH. Total polyphenols, total flavonoid contents and antioxidant activity of Korean natural medicinal plants. Korean J. Food Sci. Technol. 44: 337-342 (2012a) https://doi.org/10.9721/KJFST.2012.44.3.337
  9. Kim DS, Lee KB. Physiological characteristics and manufacturing of the processing products of sprout vegetables. Korean J. Food Cookery Sci. 26: 238-245 (2010)
  10. Kim MS, Lee YS, Kwon HY, Kim JS, Sohn HY. Antioxidative, antimicrobial, and anti-proliferative activities of the floret and stalk of broccoli (Brassica oleracea L.). Korean J. Microbiol. Biotechnol. 42: 58-66 (2014) https://doi.org/10.4014/kjmb.1401.01003
  11. Kim HS, Lee IS, Lee YJ, J Lee E, Choi MO. Antioxidative nutrients and nitrite scavenging ability of various vegetable seed sprouts. J. Hum. Ecol. 12: 109-117 (2008a) https://doi.org/10.1080/09709274.2001.11907589
  12. Kim MY, Segun P, Ahn JK, Kim JI, Chun SC, Kim EH, Seo SH, Kang EY, Kim SL, Park YJ, Ro HM, Chung IM. Phenolic compound concentration and antioxidant activities of edible and medicinal mushrooms from Korea. J. Agric. Food Chem. 56: 7265-7270 (2008b) https://doi.org/10.1021/jf8008553
  13. Kim JP, Yang YS, Kim JH, Lee HH, Kim ES, Moon YW, Kim JY, Chung JK. Chemical properties and DPPH radical scavenging ability of sword bean (Canavalia gladiata) extract. Korean J. Food Sci. Technol. 44: 441-446 (2012b) https://doi.org/10.9721/KJFST.2012.44.4.441
  14. Kim YE, Yang JW, Lee CH, Kwon EK. ABTS radical scavenging and anti-tumor effects of Tricholoma matsutake Sing. (pine mushroom). J. Korean Soc. Food Sci. Nutr. 38: 555-560 (2009) https://doi.org/10.3746/jkfn.2009.38.5.555
  15. Lee HJ. Antioxidant activity of sesamum (Sesamum indicum L.) sprouts at different growth stages. MS thesis. Kyungpook National University, Daegu, Korea (2018)
  16. Lee JM, Chang PS, Lee JH. Comparison of oxidative stability for the thermally-oxidized vegetable oils using a DPPH method. Korean J. Food Sci. Technol. 39: 133-137 (2007)
  17. Lee KI, Hwang YJ, Ban HJ, Lim SJ. Impact of the growth of single-person households on the food market and policy tasks. Korea Rural Economic Institute, Naju, Korea (2015)
  18. Lee EH, Kim CJ. Nutritional changes of buckwheat during germination. J. Korean Soc. Food Cult. 23: 121-129 (2008)
  19. Lee YJ, Kim JH, J Oh W, Shin GH, Lee JS, Cho JH, Park JJ, Lim JH, Lee OH. Antioxidant and anti-adipogenic effects of kohlrabi and radish sprout extracts. Korean J. Food Sci. Technol. 46: 531-537 (2014) https://doi.org/10.9721/KJFST.2014.46.5.531
  20. Lim SY. Changes in sulforaphane and sulforaphene content during their growth and development from Baemuchae (Brassica raphanus) and its parental lines. MS thesis. Chungang University, Seoul, Korea (2007)
  21. Lim SJ. Effects of blueberry leaves from different harvest periods on skin whitening, anti-wrinkle and antioxidant activities. MS thesis. Dankook University, Cheonan, Korea (2016)
  22. Meyers KJ, Watkins CB, Marvin PP, Liu RH. Antioxidant and antiproliferative activities of strawberries. J. Agric. Food Chem. 51: 6887-6892 (2003) https://doi.org/10.1021/jf034506n
  23. Pajak P, Socha R, Galkowska D, Roznowski J, Fortuna T. Phenolic profile and antioxidant activity in selected seeds and sprouts. Food Chem. 143: 300-306 (2014) https://doi.org/10.1016/j.foodchem.2013.07.064
  24. Pasko P, Barton H, Zagrodzki P, Pawel Z, Gorinstein S, Folta M, Zachwieja Z. Anthocyanins, total polyphenols and antioxidant activity in amaranth and quinoa seeds and sprouts during their growth. Food Chem. 115: 994-998 (2009) https://doi.org/10.1016/j.foodchem.2009.01.037
  25. Shin GH, Lee YJ, Kim JH, Kim YH, Kim DB, Lee JS, Lim JH, Lee OH. Antioxidant activities of commonly used Brassica spp. sprout vegetables in Korea. Korean J. Food Preserv. 21: 587-592 (2014) https://doi.org/10.11002/kjfp.2014.21.4.587
  26. Shin YJ, Ryu JA, Liu RH, Nock JF, Polar-cabera K, Watkins CB. Fruit quality, antioxidant contents and activity and antiproliferative activity of strawberry fruit stored in elevated $CO_2$ atmospheres. J. Food Sci. 73: 339-344 (2008)
  27. Singleton VL, Rudolf O, Rosa ML-R. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods Enzymol. 299: 152-177 (1999) https://doi.org/10.1016/S0076-6879(99)99017-1
  28. Sun J, Chu YF, Wu X, Liu RH. Antioxidant and antiproliferative activities of common fruits. J. Agr. Food Chem. 50: 7449-7454 (2002) https://doi.org/10.1021/jf0207530
  29. Thaipong K, Boonprakob U, Crosby K, Cisneros-Zevallos L, Byrne DH. Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts. J. Food Compos. Anal. 19: 669-675 (2006) https://doi.org/10.1016/j.jfca.2006.01.003
  30. Waje CK, Jun SY, Lee YK, Moon KD, Choi YH, Kwon JH. Seed viability and functional properties of broccoli sprouts during germination and postharvest storage as affected by irradiation of seeds. J. Food Sci. 74: C370-C374 (2009) https://doi.org/10.1111/j.1750-3841.2009.01161.x
  31. Wieslaw W, Dorota SN, Henryk D, Joanna M, Marcin H. Comparison of flavonoids profile in sprouts of common buckwheat cultivars and wild tartary buckwheat. Int. J. Food Sci. Technol. 49: 1977-1984 (2014) https://doi.org/10.1111/ijfs.12484
  32. Woo N, Song ES, Kim HJ, Seo MS, Kim AJ. The comparison of antioxidative activities of sprouts extract. Korean J. Food Nutr. 20: 356-362 (2007)