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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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2-O-digalloyl-1,3,4,6-tetra-O-galloyl-β-D-glucose isolated from Galla Rhois suppresses osteoclast differentiation and function by inhibiting NF-κB signaling

  • Ihn, Hye Jung (Institute for Hard Tissue and Bio-tooth Regeneration (IHBR), Kyungpook National University) ;
  • Kim, Tae Hoon (Department of Food Science and Biotechnology, Daegu University) ;
  • Kim, Kiryeong (Department of Oral Pathology and Regenerative Medicine, School of Dentistry, IHBR, Kyungpook National University) ;
  • Kim, Gi-Young (Department of Marine Life Sciences, School of Marine Biomedical Sciences, Jeju National University) ;
  • Jeon, You-Jin (Department of Marine Life Sciences, School of Marine Biomedical Sciences, Jeju National University) ;
  • Choi, Yung Hyun (Department of Biochemistry, College of Korean Medicine, Dong-Eui University) ;
  • Bae, Jong-Sup (College of Pharmacy, CMRI, Research Institute of Pharmaceutical Sciences, Kyungpook National University) ;
  • Kim, Jung-Eun (Department of Molecular Medicine, School of Medicine, Kyungpook National University) ;
  • Park, Eui Kyun (Department of Oral Pathology and Regenerative Medicine, School of Dentistry, IHBR, Kyungpook National University)
  • Received : 2019.03.05
  • Accepted : 2019.04.25
  • Published : 2019.06.30
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Abstract

Natural compounds isolated from medicinal herbs and plants have immense significance in maintaining bone health. Hydrolysable tannins have been shown to possess a variety of medicinal properties including antiviral, anticancer, and anti-osteoclastogenic activities. As a part of a study on the discovery of alternative agent against skeletal diseases, we isolated a hydrolysable tannin, 2-O-digalloyl-1,3,4,6-tetra-O-galloyl-${\beta}$-D-glucose (DTOGG), from Galla Rhois and examined the effect on osteoclast formation and function. We found that DTOGG significantly inhibited receptor activator of nuclear factor-${\kappa}B$ ligand (RANKL)-induced osteoclast differentiation by downregulating the expression of the key regulator in osteoclastogenesis as well as osteoclast-related genes. Analysis of RANKL/RANK signaling revealed that DTOGG impaired activation of $I{\kappa}B{\alpha}$ and p65 in the nuclear factor kappa-lightchain-enhancer of activated B cells (NF-${\kappa}B$) signaling pathway. Furthermore, DTOGG reduced bone resorbing activity of osteoclasts, compared to the vehicle-treated control. These results suggest that DTOGG could be a useful natural compound to manage osteoclast-mediated skeletal diseases.

Keywords

References

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