Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Comparison of Anti-oxidant and Anti-inflammatory Activities of Methanolic Extracts Obtained from Different Parts of Cotoneaster wilsonii Nakai

섬개야광나무의 부위별 메탄올 추출물의 항산화 및 항염 활성 비교

  • Yoo, Nam Ho (Division of Bio-Resource Sciences, Kangwon National University) ;
  • Kim, Hee Kyu (Gangwondo Forest Science Institute) ;
  • Lee, Chan Ok (Gangwondo Forest Science Institute) ;
  • Park, Ju Hee (Lambio Institute, Kangwon National University) ;
  • Kim, Myong Jo (Division of Bio-Resource Sciences, Kangwon National University)
  • 유남호 (강원대학교 생물자원과학부) ;
  • 김희규 (강원도산림과학연구원) ;
  • 이찬옥 (강원도산림과학연구원) ;
  • 박주희 ((주)램바이오 연구소) ;
  • 김명조 (강원대학교 생물자원과학부)
  • Received : 2019.04.16
  • Accepted : 2019.06.09
  • Published : 2019.06.30
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Abstract

Background: The Ministry of Environment, Korea recognizes Cotoneaster wilsonii Nakai as a second-class endangered plant. It is a native species that grows in Ulleung-do, Korea. To our knowledge, the bioactivity of this plant has not yet been reported. Therefore, in this study, we have reported the bioactivity of C. wilsonii Nakai. Methods and Results: The anti-oxidant activities of C. wilsonii methanolic extract were investigated in vitro. The anti-oxidant activity was evaluated using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical assay, and the total phenolic and flavonoid content were measured. The leaf methanolic extract had the highest DPPH radical scavenging activity ($IC_{50}$; $15.74{\mu}g/m{\ell}$), and it also had the highest total phenolic and flavonoid content ($220.95mg{\cdot}GAE/g$, and $36.46mg{\cdot}QE/g$ extract respectively). Through high performance liquid chromatography (HPLC) analysis, chlorogenic acid was found to be the predominant among all phenolic compounds, showing a concentration of 84.24 mg/g extract. More than 60% decrease in nitric oxide (NO) production was found in the leaf methanolic extract. Conclusions: To the best of our knowldege, this is the first report of the bioactivities of C. wilsonii. The results demonstrate that the leaf methanolic extract of C. wilsonii shows potent anti-oxidant, and anti-inflammatory activities.

Keywords

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Fig. 1. Reducing power of extractions from C. wilsonii Nakai.

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Fig. 2. Inhibitory effect of extract from C. wilsonii Nakai on cytotoxicity of RAW264.7 cell.

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Fig. 3. The effect of C. wilsonii Nakai on LPS-induced NO production in RAW264.7 cell.

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Fig. 4. HPLC chromatograms of standard compounds and methanolic extracts from C. wilsonii Nakai.

Table 1. DPPH radical scavenging activity of extractions from C. wilsonii Nakai.

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Table 2. The total phenolic and flavonoid contents of extractions from C. wilsonii Nakai.

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Table 3. Calibration curve of standard and limit of detection (LOD) and, limit of quantitation (LOQ).

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Table 4. The difference in the content of phenolic acid composition of the C. wilsonii Nakai.

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