Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Enhancement of Respiratory Protective and Therapeutic Effect of Salvia plebeia R. Br. Extracts in Combination with Korean Red Ginseng

배암차즈기와 홍삼 복합물의 호흡기 보호 및 질환 치료 상승 효과

  • Received : 2019.04.12
  • Accepted : 2019.06.04
  • Published : 2019.06.30
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Abstract

Background: We recently reported that Salvia plebeia R. Br. extracts suppress leukotriene production and effectively inhibit the airway inflammatory response by modulating inflammatory chemokine and cytokine expression. Here, we investigated the synergistic airway anti-inflammation effect of Salvia plebeia and Panax ginseng (Korean red ginseng, KRG) that has been used to treat various immune diseases such as asthma. Methods and Results: To evaluate the synergistic airway anti-inflammatory effect of Salvia plebeia and KRG, we measured the inhibitory effect of monotheraphy with either or co-theraphy with both on leukotriene and reactive oxygen species (ROS) production. Using coal a combustion, fly ash, and diesel exhaust particle (CFD)-induced respiratory disease mouse model, we found that co-theraphy synergistically suppressed airway inflammatory signs such as alveolar wall thickness and collagen fibers deposition, and decreased the number of total cell, $CD11b^+Gr-1^+$ cells, and inflammatory cytokines (IL17A, TNF, MIP-2 and CXCL-1) in bronchoalveolar lavage (BAL) fluid. Conclusions: We confirmed respiratory protection as a therapeutic effect of the Salbia plebeia-KRG 3 : 1 complex (KGC-03-PS) via anti-tracheal muscle contraction and expectorant animal studies using a CFD-induced respiratory disease mouse model.

Keywords

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Fig. 1. Chromatogram and structure of four compounds purified from KGC-03-PS (KGC3P).

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Fig. 2. Determination of optical ratio between Korean red ginseng and Salvia plebeia R. Br on suppression of creating radical and leukotriene.

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Fig. 3. Experimental schedule to make coal combustion-fly ash-diesel exhaust particles complex (CFD)-induced mice model and Histopathology.

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Fig. 4. The Effect of Salvia plebeia, Korean red ginseng and complexs on the infiltration of granulocytes in airway from coal combustion-fly ash-diesel exhaust particles complex (CFD)-induced mice model.

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Fig. 5. The Effect of Salvia plebeia, Korean red ginseng and complexs on pro-inflammatory cytokine in BALF.

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Fig. 6. The restoration of airway immune response associated chemokines and inflammatory cytokines by Salvia plebeia, Korean red ginseng and complexs in coal combustion-fly ash-diesel exhaust particles complex (CFD)-induced mice models.

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Fig. 7. The effect of KGC3P on airway immune cell number and neutrophilic airway inflammation in coal combustion-fly ashdiesel exhaust particles complex (CFD)-induced airway inflammation mice model.

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Fig. 8. The dose dependent reduced production of ROS by KGC3P in BAL fluid of coal combustion-fly ash-diesel exhaust particles complex (CFD)-induced mice models.

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Fig. 9. Effect of Salvia, Korea red ginseng and KGC-03-PS on the amount of tracheal phenol red secretion in mice.

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Fig. 10. Effect of KGC-03-PS on carbachol-induced contraction of isolated trachea from guinea pigs.

Table 1. Mouse oligonucleatide primer sequence.

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