Journal of Integrative Natural Science (통합자연과학논문집)

The Basic Science Institute Chosun University (조선대학교 기초과학연구원)
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Fabrication of Antimicrobial Wound Dressings Using Silver-Citrate Nanorods and Analysis of Their Wound-Healing Efficacy

  • Park, Yong Jin (Department of Medicine, College of Medicine, Chosun University) ;
  • Jeong, Jisu (Department of Chemistry, College of Natural Science, Chosun University) ;
  • Kim, Jae Seok (Department of Chemistry, College of Natural Science, Chosun University) ;
  • Choi, Dong Soo (Post-Harvest Engineering Division, National Institute of Agricultural Sciences) ;
  • Cho, Goang-Won (Department of Biology, College of Natural Science, Chosun University) ;
  • Park, Jin Seong (Department of Materials Engineering, College of Engineering, Chosun University) ;
  • Lim, Jong Kuk (Department of Chemistry, College of Natural Science, Chosun University)
  • Received : 2019.05.23
  • Accepted : 2019.06.24
  • Published : 2019.06.30
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Abstract

Staphylococcus epidermidis is well-known not only as an innocuous normal flora species commonly isolated from human skin, but also as an important bacterial species to keep skin healthy, because this species can protect the human skin from pathogenic microorganisms. However, S. epidermidis turns into a potential pathogen in damaged skin, because these bacteria can easily form a biofilm on the wound area and provide antimicrobial resistance to other microorganisms embedded in the biofilm. Thus, it is important to kill S. epidermidis in the early stage of wound treatment and block the formation of biofilms in advance. In the present study, hydrogel wound dressings were fabricated using polyvinyl alcohol/polyethylene glycol containing silver citrate nanorods, which have been proven to have strong antimicrobial activity, especially against S. epidermidis, and their wound-healing efficacy was investigated in vivo using a rat experiment.

Keywords

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Fig. 1. Morphology of silver citrate compounds prepared in different experimental conditions.

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Fig. 2. Morphology of silver citrate compounds prepared in different experimental conditions.

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Figure 3. Six different hydrogels (a-f) were prepared using 5 or 10% of polyvinyl alcohol (PVA) with 10, 20, or 40% polyethylene glycol (PEG). Hydrogels (a, b, and c) made with 5% PVA were so weak, regardless of PEG concentration, that they were easily torn by hand. Hydrogels (d, e, and f) containing 10% PVA did not tear as easily as hydrogels made with 5% PVA, but when the concentration of PEG reached 40%, they became too hard and were easily broken. The Young’s modulus, tensile strength, and percent elongation of the hydrogel were measured by a texture analyzer. (g) Representative stressstrain curve of hydrogels prepared with different PEG concentrations.

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Figure 4. (a) Average percent elongation and tensile strength and (b) gel fraction and swelling ratio depended on the PEG concentration.

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Figure 6. Clinical Rat Experiment.

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Figure 5. Cytotoxicity of bulk silver citrates and silver citrate nanorods in NIH3T3 and HS68 cells.

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