Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
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Toxic evaluation of phenanthrene and zinc undecylenate using the population growth rates of marine diatom, Skeletonema costatum

해산규조류(Skeletonema costatum)의 개체군 성장률을 이용한 phenanthrene와 zinc undecylenate의 독성평가

  • Lee, Ju-Wook (Marine Ecological Risk Assessment Center, West Sea Fisheries Research Institute, National Institute of Fisheries Science) ;
  • Choi, Hoon (Marine Ecological Risk Assessment Center, West Sea Fisheries Research Institute, National Institute of Fisheries Science) ;
  • Park, Yun-Ho (Marine Ecological Risk Assessment Center, West Sea Fisheries Research Institute, National Institute of Fisheries Science) ;
  • Lee, Yoon (Marine Ecological Risk Assessment Center, West Sea Fisheries Research Institute, National Institute of Fisheries Science) ;
  • Heo, Seung (Marine Ecological Risk Assessment Center, West Sea Fisheries Research Institute, National Institute of Fisheries Science) ;
  • Hwang, Un-Ki (Marine Ecological Risk Assessment Center, West Sea Fisheries Research Institute, National Institute of Fisheries Science)
  • 이주욱 (국립수산과학원 서해수산연구소 자원환경과 해양생태위해평가센터) ;
  • 최훈 (국립수산과학원 서해수산연구소 자원환경과 해양생태위해평가센터) ;
  • 박윤호 (국립수산과학원 서해수산연구소 자원환경과 해양생태위해평가센터) ;
  • 이윤 (국립수산과학원 서해수산연구소 자원환경과 해양생태위해평가센터) ;
  • 허승 (국립수산과학원 서해수산연구소 자원환경과 해양생태위해평가센터) ;
  • 황운기 (국립수산과학원 서해수산연구소 자원환경과 해양생태위해평가센터)
  • Received : 2019.09.02
  • Accepted : 2019.09.16
  • Published : 2019.09.30
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Abstract

We evaluated the toxic effects of phenanthrene (PHE) and zinc undecylenate (ZU) on the population growth rate (r) of the marine diatom, Skeletonema costatum. The r of S. costatum was determined after 96 hrs of exposure to PHE (0, 25, 50, 100, 200 and 300 mg L-1) and ZU (0, 5, 10, 15, 20 and 25 mg L-1). The results showed that r in the control (the absence of PHE and ZU) was greater than 0.04, while r in the treatment groups decreased with increasing PHE and ZU concentrations. PHE and ZU were shown to reduce r in a dose-dependent manner, with significant decreases occurring at concentrations above 50 and 10 mg L-1, respectively. The EC50 values of r in PHE and ZU exposure were 136.13 and 16.95 mg L-1, respectively. The no observed effect concentrations (NOEC) were 25 and 5 mg L-1, and the lowest observed effect concentrations (LOEC) were 50 and 10 mg L-1. These results indicated that concentrations of greater than 50 mg L-1 of PHE and 10 mg L-1 of ZU in marine ecosystems induced a toxic effect on the r of S. costatum. These results can serve as useful baseline data for the establishment of safety concentrations of PHE and ZU in marine ecosystems.

해산규조류(Skeletonema cosatatum)의 개체군성장률(r)을 사용하여 연안 유기오염물질인 phenanthrene (PHE)과 zinc undecylenate (ZU)의 독성평가를 실시하였다. S. costatum을 PHE(0, 25, 50, 100, 200, 300 mg L-1)와 ZU(0, 5, 10, 15, 20, 25 mg L-1)에 각각 96시간 노출한 이후에 r을 산출하였고, 대조구의 r은 0.04보다 높아 시험기준에 적합하였다. S. costatum의 r은 PHE 50, ZU 10mg L-1 이상의 농도에서 대조구 대비 유의하게 감소하기 시작해 PHE와 ZU의 농도가 증가할수록 감소되는 농도의존성을 나타냈으며, 최고농도인 300과 25mg L-1 농도에서는 r이 나타나지 않았다. PHE와 ZU에 노출된 S. costatum r의 반수영향농도(EC50)은 136.13, 16.95 mg L-1, 무영향농도(NOEC)는 25, 5 mg L-1, 최소영향농도(LOEC)는 50, 10 mg L-1로 나타났다. 본 연구결과, 해양생태계 내에서 S. costatum의 r은 PHE 50 mg -1, ZU 10 mg L-1 이상의 농도에서 독성영향으로 감소할 것으로 판단되며, PHE와 ZU의 기준농도 설정을 위한 기초자료로 유용하게 사용될 것이다.

Keywords

References

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