The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Identification of Proteomic Components Associated with Resistance to Fusarium Head Blight in Rye

  • Perlikowski, Dawid (Institute of Plant Genetics, Polish Academy of Sciences) ;
  • Wisniewska, Halina (Institute of Plant Genetics, Polish Academy of Sciences) ;
  • Goral, Tomasz (Plant Breeding and Acclimatization Institute - National Research Institute) ;
  • Ochodzki, Piotr (Plant Breeding and Acclimatization Institute - National Research Institute) ;
  • Majka, Maciej (Institute of Plant Genetics, Polish Academy of Sciences) ;
  • Pawlowicz, Izabela (Institute of Plant Genetics, Polish Academy of Sciences) ;
  • Belter, Jolanta (Institute of Plant Genetics, Polish Academy of Sciences) ;
  • Kosmala, Arkadiusz (Institute of Plant Genetics, Polish Academy of Sciences)
  • Received : 2018.11.29
  • Accepted : 2019.04.09
  • Published : 2019.08.01
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Abstract

Rye was used here to dissect molecular mechanisms of resistance to Fusarium head blight (FHB) and to go deeper with our understanding of that process in cereals. F. culmorum-damaged kernels of two lines different in their potential of resistance to FHB were analyzed using two-dimensional gel electrophoresis and mass spectrometry to identify resistance markers. The proteome profiling was accompanied by measurements of ${\alpha}-$ and ${\beta}-amylase$ activities and mycotoxin content. The proteomic studies indicated a total of 18 spots with clear differences in protein abundance between the more resistant and more susceptible rye lines after infection. Eight proteins were involved in carbohydrate metabolism of which six proteins showed a significantly higher abundance in the resistant line. The other proteins recognized here were involved in stress response and redox homeostasis. Three remaining proteins were associated with protease inhibition/resistance and lignin biosynthesis, revealing higher accumulation levels in the susceptible rye line. After inoculation, the activities of ${\alpha}-$ and ${\beta}-amylases$, higher in the susceptible line, were probably responsible for a higher level of starch decomposition after infection and a higher susceptibility to FHB. The presented results could be a good reference for further research to improve crop resistance to FHB.

Keywords

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Fig. 1. The single representative 2-DE protein maps of rye kernels in the control conditions (C) and after Fusarium culmorum infection (FDK - Fusarium-damaged kernels) 2-dimensional electrophoresis for the line more resistant (R) and more susceptible (S) to Fusarium head blight. The spots with differentially accumulated (P ≤ 0.05) proteins (1-18) between both analyzed lines, are numbered.

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Fig. 2. Comparison of α-amylase (A) and β-amylase (B) activity in the kernels of rye S (line more susceptible to Fusarium head blight) and R (line more resistant to Fusarium head blight) after Fusarium culmorum infection (FDK – Fusarium-damaged kernels) and in control conditions (HLK – healthy looking kernels). The enzyme activity was expressed in Ceralpha Units (CU) per gram of flour. The means of three biological replicates and standard deviation bars are shown. Values marked with the same letter did not differ at a significance level of 0.05 (Fisher’s LSD-test).

Table 1. The components of the resistance to Fusarium head blight in the more resistant (R) and more susceptible (S) rye lines and their yields under control conditions

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Table 2. Fusarium culmorum biomass in the kernels of more resistant (R) and more susceptible (S) rye lines [mg/g]

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Table 3. The results of MS analysis performed on the spots that showed at least a 2.0 ratio (P ≤ 0.05) in protein abundance between the more resistant and more susceptible rye lines

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References

  1. Augustyniak, A., Perlikowski, D., Rapacz, M., Koscielniak, J. and Kosmala, A. 2018. Insight into cellular proteome of Lolium multiflorum/Festuca arundinacea introgression forms to decipher crucial mechanisms of cold acclimation in forage grasses. Plant Sci. 272:22-31. https://doi.org/10.1016/j.plantsci.2018.04.002
  2. Bottalico, A. 1998. Fusarium diseases of cereals: Species complex and related mycotoxin profiles, in Europe. J. Plant Pathol. 80:85-103.
  3. Bottalico, A. and Perrone, G. 2002. Toxigenic Fusarium species and mycotoxins associated with head blight in small-grain cereals in Europe. Eur. J. Plant Pathol. 108:611-624. https://doi.org/10.1023/A:1020635214971
  4. Buerstmayr, H., Ban, T. and Anderson, J. A. 2009. QTL mapping and marker-assisted selection for Fusarium head blight resistance in wheat: A review. Plant Breed. 128:1-26. https://doi.org/10.1111/j.1439-0523.2008.01550.x
  5. Chakraborty, S., Liu, C. J., Mitter, V., Scott, J. B., Akinsanmi, O. A., Ali, S., Dill-Macky, R., Nicol, J., Backhouse, D. and Simpfendorfer, S. 2006. Pathogen population structure and epidemiology are keys to wheat crown rot and Fusarium head blight management. Aust. Plant Pathol. 35:643-655. https://doi.org/10.1071/AP06068
  6. Chelkowski, J., Kaptur, P., Tomkowiak, M., Kostecki, M., Golinski, P., Ponitka, A., Slusarkiewicz-Jarzina, A. and Bocianowski, J. 2000. Moniliformin accumulation in kernels of triticale accessions inoculated with Fusarium avenaceum, in Poland. J. Phytopathol. 148:433-439. https://doi.org/10.1046/j.1439-0434.2000.00538.x
  7. Cowger, C., Patton-Ozkurt, J., Brown-Guedira, G. and Perugini, L. 2009. Post-anthesis moisture increased Fusarium head blight and deoxynivalenol levels in North Carolina winter wheat. Phytopathology 99:320-327. https://doi.org/10.1094/PHYTO-99-4-0320
  8. Desjardins, A. E. 2006. Fusarium mycotoxins. chemistry, genetics, and biology. APS Press, St. Paul, MN, USA. 260 pp.
  9. Gerlach, W. and Nirenberg, H. 1982. The genus Fusarium-a pictorial atlas. Mitt. Biol. Bundesanst. Land Forstwirtsch. Berl. Dahlem 209:1-406.
  10. Goral, T., Wisniewska H., Ochodzki, P. and Walentyn-Goral, D. 2016. Higher Fusarium toxin accumulation in grain of winter triticale lines inoculated with Fusarium culmorum as compared with wheat. Toxins 8:301. https://doi.org/10.3390/toxins8100301
  11. Goral, T., Wisniewska, H., Ochodzki, P., Nielsen, L. K., Walentyn-Goral, D. and Stepien, L. 2019. Relationship between Fusarium head blight, kernel damage, concentration of Fusarium biomass, and Fusarium toxins in grain of winter wheat inoculated with Fusarium culmorum. Toxins 11:E2.
  12. Kiecana, I. and Mielniczuk, E. 2010. Fusarium head blight of winter rye (Secale cereale L.) Irena. Acta Agrobot. 63:129-135. https://doi.org/10.5586/aa.2010.015
  13. Kosmala, A., Bocian, A., Rapacz, M., Jurczyk, B. and Zwierzykowski, Z. 2009. Identification of leaf proteins differentially accumulated during cold acclimation between Festuca pratensis plants with distinct levels of frost tolerance. J. Exp. Bot. 60:3595-3609. https://doi.org/10.1093/jxb/erp205
  14. Kosmala, A., Perlikowski, D., Pawlowicz, I. and Rapacz, M. 2012. Changes in the chloroplast proteome following water deficit and subsequent watering in a high and a low drought tolerant genotype of Festuca arundinacea. J. Exp. Bot. 63:6161-6172. https://doi.org/10.1093/jxb/ers265
  15. Langevin, F., Eudes, F. and Comeau, A. 2004. Effect of trichothecenes produced by Fusarium graminearum during Fusarium head blight development in six cereal species. Eur. J. Plant Pathol. 110:735-746. https://doi.org/10.1023/B:EJPP.0000041568.31778.ad
  16. Lees, A. K., Nicholson, P., Rezanoor, H. N. and Parry, D. W. 1995. Analysis of variation within Microdochium nivale from wheat: evidence for a distinct sub-group. Mycol. Res. 99:103-109. https://doi.org/10.1016/S0953-7562(09)80322-5
  17. Marin, S., Ramos, A. J., Cano-Sancho, G. and Sanchis, V. 2013. Mycotoxins: occurrence, toxicology, and exposure assessment. Food Chem. Toxicol. 60:218-37. https://doi.org/10.1016/j.fct.2013.07.047
  18. Mesterhazy, A. 1995. Types and components of resistance to Fusarium head blight of wheat. Plant Breed. 114:377-386. https://doi.org/10.1111/j.1439-0523.1995.tb00816.x
  19. Miedaner, T. 1997. Breeding wheat and rye for resistance to Fusarium diseases. Plant Breed. 116:201-220. https://doi.org/10.1111/j.1439-0523.1997.tb00985.x
  20. Miedaner, T., Reinbrecht, C., Lauber, U., Schollenberger, M. and Geiger, H. H. 2001. Effects of genotype and genotypeenvironment interaction on deoxynivalenol accumulation and resistance to Fusarium head blight in rye, triticale, and wheat. Plant Breed. 120:97-105. https://doi.org/10.1046/j.1439-0523.2001.00580.x
  21. Miedaner, T., Schneider, B. and Geiger, H. H. 2003. Deoxynivalenol (DON) content and fusarium head blight resistance in segregating populations of winter rye and winter wheat. Crop Sci. 43:519-526. https://doi.org/10.2135/cropsci2003.0519
  22. Muthomi, J. W., Ndung'u, J. K., Gathumbi, J. K., Mutitu, E. W. and Wagacha, J. M. 2008. The occurrence of Fusarium species and mycotoxins in Kenyan wheat. Crop Prot. 27:1215-1219. https://doi.org/10.1016/j.cropro.2008.03.001
  23. Parry, D. W., Jenkinson, P. and McLeod, L. 1995. Fusarium ear blight (scab) in small grain cereals-a review. Plant Pathol. 44:207-238. https://doi.org/10.1111/j.1365-3059.1995.tb02773.x
  24. Perlikowski, D., Wisniewska, H., Goral, T., Kwiatek, M., Majka, M. and Kosmala, A. 2014. Identification of kernel proteins associated with the resistance to Fusarium head blight in winter wheat (Triticum aestivum L.). PLoS One 9:e110822. https://doi.org/10.1371/journal.pone.0110822
  25. Perlikowski, D., Wisniewska, H., Kaczmarek, J., Goral, T., Ochodzki, P., Kwiatek, M., Majka, M., Augustyniak, A. and Kosmala, A. 2016. Alterations in kernel proteome after infection with Fusarium culmorum in two triticale cultivars with contrasting resistance to Fusarium head blight. Front. Plant Sci. 7:1217.
  26. Pirgozliev, V. R., Birch, C. L., Rose, S. P., Kettlewell, P. S. and Bedford, M. R. 2003. Chemical composition and the nutritive quality of different wheat cultivars for broiler chickens. Br. Poult. Sci. 44:464-475. https://doi.org/10.1080/0007166031000085594
  27. Snijders, C. H. A. 2004. Resistance in wheat to Fusarium infection and trichothecene formation. Toxicol. Lett. 153:37-46. https://doi.org/10.1016/j.toxlet.2004.04.044
  28. Srobarova, A., Slikova, S. and Sudyova, V. 2008. Diversity of the Fusarium species associated with head and seedling blight on wheat in Slovakia. Biologia 63:332-337. https://doi.org/10.2478/s11756-008-0050-y
  29. Wang, J., Pawelzik, E., Weinert, J. and Wolf, G. A. 2005. Impact of Fusarium culmorum on the polysaccharides of wheat flour. J. Agric. Food Chem. 53:5818-5823. https://doi.org/10.1021/jf050525g
  30. Xu, X. M., Parry, D. W., Nicholson, P., Thomsett, M. A., Simpson, D., Edwards, S. G., Cooke, B. M., Doohan, F. M., Brennan, J. M., Moretti, A., Tocco, G., Mule, G., Hornok, L., Giczey, G. and Tatnell, J. 2005. Predominance and association of pathogenic fungi causing Fusarium ear blightin wheat in four European countries. Eur. J. Plant Pathol. 112:143-154. https://doi.org/10.1007/s10658-005-2446-7