The Journal of the Convergence on Culture Technology (문화기술의 융합)

The International Promotion Agency of Culture Technology (국제문화기술진흥원)
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The Effects of Heated Radish Extract on the Prevention of Ulcerative Colitis Inflammation

열처리된 무 추출물의 궤양성 대장염증 예방 효과에 미치는 영향

  • 김현경 (서원대학교 식품공학과)
  • Received : 2019.04.18
  • Accepted : 2019.06.08
  • Published : 2019.08.31
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Abstract

The present study aimed to investigate the comparative evaluation of pharmacological efficacy between sulfasalazine alone and combination with herbal medicine on dextran sodium sulfate (DSS)-induced UC in mice. Balb/c mice received 5% DSS in drinking water for 7 days to induce colitis. Animals were divided into five groups (n = 9): group I-normal group, group II-DSS control group, group III-DSS + sulfasalazine (30 mg/kg), group IV-DSS + sulfasalazine (60 mg/kg), group V-DSS + sulfasalazine (30 mg/kg) + Radish Extract mixture (30 mg /kg) (SRE). DSS-treated mice developed symptoms similar to those of human UC, such as severe bloody diarrhea and weight loss. SRE supplementation, as well as sulfasalazine, suppressed colonic length and mucosal inflammatory infiltration. In addition, SRE treatment significantly reduced the expression of pro-inflammatory signaling molecules through suppression both MAPK) and nuclear factor-kappa B (NF-${\kappa}B$) signaling pathways, and prevented the apoptosis of colon. Moreover, SRE administration significantly led to the up-regulation of anti-oxidant enzyme including SOD and Catalase. This is the first report that Radish extract mixture combined with sulfasalazine protects against experimental UC via the inhibition of both inflammation and apoptosis, very similar to the standard-of-care sulfasalazin.

본 연구는 인체에 유익한 효과가 있고 부작용 없이 장기간 안전하게 사용할 수 있으며 표준치료제인 설파살라진과 비교하여 열처리된 무 추출물의 궤양성 대장염증의 예방에 탁월한 효과가 있는지 알아보았다. 쥐에 덱스트란나트륨설페이트(DSS)로 유도된 UC에 대한 설파살라진 투여군과 열처리된 무추출물 투여군에 대해 약리효능의 비교 평가를 알아보았다. Balb/c 마우스는 7일동안 식수에 5 % DSS를 투여하여 대장염을 유도하였다. DSS+ sulfasalazine(30 mg/kg), IV-DSS + sulfasalazine(60 mg/kg), 5-DSS +, V-DSS + 설파살라진(30 mg/kg) + 무우 엑기스 혼합물(30 mg/kg) (SRE). DSS 처리된 마우스는 심한 피의 설사 및 체중 감소와 같은 인간 UC와 유사한 증상을 보였다. 설파살라진 뿐만 아니라 SRE보충은 결장길이와 점막염증 침투를 억제하였다. 또한 SRE 치료는 MAPK와 nuclear factor-kappa B(NF-${\kappa}B$) 신호전달 경로를 억제함으로써 전염증 신호분자의 발현을 현저히 감소 시켰으며 결장의 세포 사멸을 예방하였다. 또한, SRE 투여는 SOD 및 카탈라아제를 포함한 항산화 효소의 상향 조절을 의미있게 만들었다. 이번 연구 결과는 열처리된 무추출물과 설파살라진의 병용 투여가 sulfasalazin 표준과 매우 유사한 염증과 세포사멸의 억제. 열처리된 무추출물은 궤양성 대장염 유발에 의한 대장의 길이 감소와 조직학적 변화에 의한 대장 길이의 감소나 조직학적 변화를 효과적으로 억제 하였다.

Keywords

References

  1. Kim HY, "The effects of fruit and vegetable bark extract on learning ability and memory improvement," The Journal of the Convergence on Culture Technology, Vol. 4(3), pp. 261-267, 2018. http://dx.doi.org/10.17703/JCCT.2018.4.3.261.
  2. Kim DB, Ahn EY and Kim EJ, "Improvement of insulin resistance by curcumin in high fat diet fed mice", The Journal of the Convergence on Culture Technology, Vol. 4(1), pp. 315-323, 2018. http://dx.doi.org/10.17703/JCCT.2018.4.1.315.
  3. Kim HK, "The functional effects of antimicrobaial activity and anti-inflammatory seaweed polysaccharide extracts," The Jorurnal of the Convergence on Culture Technology, Vol. 4(2), pp. 155-163, 2018. http://dx.doi.org/10.17703/JCCT.2018.4.2.155.
  4. Jung JS, Kwon J, Jung SH, Lee MW. Mariappan V and Cha JS, "Impact of SV40 T antigen on two multiple fission microalgae species Scenedesmus quadricauda and chlorella vulgaris," International Journal of Advanced Smart Convergence(IJASC), Vol. 6, No.1, pp.82-88, March 2017. https://doi.org/10.7236/IJASC.2017.6.1.82
  5. Jeon HJ, Hafeez J, Hamacher A, Lee S and Kwon SC, "A study on the quality of photometric scanning under variable illumination conditions," International Journal of advanced smart convergence(IJASC), Vol. 6, No. 4, pp. 88-95, June 2017. https://doi.org/10.7236/IJASC.2017.6.4.13
  6. Kim K, "Physiological functionalities and anti-oxidant activity of heated radish extract", International Journal of advanced smart convergence(IJASC) , Vol. 7, No. 2, pp. 38-46, June 2018. https://doi.org/10.7236/IJASC.2018.7.2.38
  7. Shailubhai K, Palejwala V, Arjunan KP, et al., "Plecanatide and dolcanatide, novel guanylate cyclase-C agonists, ameliorate gastrointestinal inflammation in experimental models of murine colitis," World journal of gastrointestinal pharmacology and therapeutics, Vol. 6, No. 4, pp. 213-222, November 2015. https://doi.org/10.4292/wjgpt.v6.i4.213
  8. Kaser A, Zeissig S and Blumberg RS, "Inflammatory bowel disease," Annual review of immunology, Vol. No. 28, pp. 573-621, January 2010. https://doi.org/10.1146/annurev-immunol-030409-101225
  9. Ordas I, Eckmann L, Talamini M, Baumgart DC and Sandborn WJ, "Ulcerative colitis," The Lancet, Vol. 380, No. 9853, pp.1606-1619, November 2012. https://doi.org/10.1016/S0140-6736(12)60150-0
  10. Ross AS, and Cohen RD, "Medical therapy for ulcerative colitis: the state of the art and beyond," Current gastroenterology reports, Vol. 6, No. 6, pp. 488-495, December 2004. https://doi.org/10.1007/s11894-004-0071-9
  11. Xiao HT, Peng J, Hu DD, et al., "Qing-dai powder promotes recovery of colitis by inhibiting inflammatory responses of colonic macrophages in dextran sulfate sodium-treated mice," Chinese medicine, Vol. 13, No. 10, pp. 29, October 2015. https://doi.org/10.1186/s13020-015-0061-x
  12. V.W. Wong, M.Y. Law, A.Y. Hui et al., "A hospital clinic-based survey on traditional Chinese medicine usage among chronic hepatitis B patients," Complementary therapies in medicine, Vol. 13, No. 3, pp. 175-182, September 2005. https://doi.org/10.1016/j.ctim.2005.04.006
  13. Hattori T, Nagamatsu T, Ito M andSuzuki Y, "Studies on antinephritic effect of TJ-8014, a new Japanese herbal medicine, and its mechanisms (1): Effects on original-type anti-GBM nephritis in rats and platelet aggregation," Japanese journal of pharmacology, vol. 52, no. 1, pp. 477-485, September 1990.
  14. Sanchez-Munoz F, Dominguez-Lopez A and Yamamoto-Furusho JK, "Role of cytokines in inflammatory bowel disease," World journal of gastroenterology, Vol. 14, No. 27, pp. 4280-4288, July 2008. https://doi.org/10.3748/wjg.14.4280
  15. B.R. Jin, K.S. Chung, S.Y. Cheon et al., "Rosmarinic acid suppresses colonic inflammation in dextran sulphate sodium (DSS)-induced mice via dual inhibition of $NF-{\kappa}B$ and STAT3 activation," Scientific reports, Vol. 7, pp. 46252, April 2017. https://doi.org/10.1038/srep46252
  16. Rogler G, "Gastrointestinal and liver adverse effects of drugs used for treating IBD," Best practice & research. Clinical gastroenterology, Vol. 24, No. 2, pp. 157-165, April 2010. https://doi.org/10.1016/j.bpg.2009.10.011
  17. Buchheister S, Buettner M, Basic M, et al., "CD14 Plays a Protective Role in Experimental Inflammatory Bowel Disease by Enhancing Intestinal Barrier Function," The American journal of pathology, Vol. 187, No. 5, pp. 1106-1120, January 2017. https://doi.org/10.1016/j.ajpath.2017.01.012
  18. Pandurangan AK, Ismail S, Saadatdoust Z, Esa NM,"Allicin Alleviates Dextran Sodium Sulfate-(DSS-) Induced Ulcerative Colitis in BALB/c Mice," Oxidative medicine and cellular longevity, vol. 2015, pp. 605208, April 2015. https://doi.org/10.1155/2015/605208
  19. Wei DD, Lin XH, Wang HC, et al., "Apocynin relieves inflammation in dextran sulfate sodium-induced ulcerative colitis mice: the role of NOXs-ROS-p38MAPK pathway," Sheng Li Xue Bao, vol. 67, no. 1, pp. 74-82, February 2015.
  20. Islam MS, Murata T, Fujisawa M, et al., "Anti-inflammatory effects of phytosteryl ferulates in colitis induced by dextran sulphate sodium in mice," British Journal of Pharmacology, Vol. 154, No. 4, pp. 812-824, June 2008. https://doi.org/10.1038/bjp.2008.137