Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
권호 표지
DOI QR코드

DOI QR Code

Eight-week healing of grafted calvarial bone defects with hyperbaric oxygen therapy in rats

  • Oh, Seo-Eun (Ministry of National Defense Agency for KIA Recovery & Identification) ;
  • Hu, Kyung-Seok (Division in Anatomy and Developmental Biology, Department of Oral Biology, Human Identification Research Institute, BK21 PLUS Project, Yonsei University College of Dentistry) ;
  • Kim, Sungtae (Department of Periodontology, Dental Research Institute, Seoul National University School of Dentistry)
  • Received : 2019.02.12
  • Accepted : 2019.07.15
  • Published : 2019.08.30
Download PDF
⟨ Previous Next ⟩

Abstract

Purpose: The purpose of this study was to evaluate the synergistic effect of adjunctive hyperbaric oxygen (HBO) therapy on new bone formation and angiogenesis after 8 weeks of healing. Methods: Sprague-Dawley rats (n=28) were split into 2 groups according to the application of adjunctive HBO therapy: a group that received HBO therapy (HBO group [n=14]) and another group that did not receive HBO therapy (NHBO group [n=14]). Each group was divided into 2 subgroups according to the type of bone graft material: a biphasic calcium phosphate (BCP) subgroup and an Escherichia coli-derived recombinant human bone morphogenetic protein-2-/epigallocatechin-3-gallate-coated BCP (mBCP) subgroup. Two identical circular defects with a 6-mm diameter were made in the right and left parietal bones of each rat. One defect was grafted with bone graft material (BCP or mBCP). The other defect was not grafted. The HBO group received 2 weeks of adjunctive HBO therapy (1 hour, 5 times a week). The rats were euthanized 8 weeks after surgery. The specimens were prepared for histologic analysis. Results: New bone (%) was higher in the NHBO-mBCP group than in the NHBO-BCP and control groups (P<0.05). Blood vessel count (%) and vascular endothelial growth factor staining (%) were higher in the HBO-mBCP group than in the NHBO-mBCP group (P<0.05). Conclusions: HBO therapy did not have a positive influence on bone formation irrespective of the type of bone graft material applied after 8 weeks of healing. HBO therapy had a positive effect on angiogenic activity.

Keywords

Acknowledgement

Supported by : Seoul National University Dental Hospital (SNUDH)

References

  1. Dauwe PB, Pulikkottil BJ, Lavery L, Stuzin JM, Rohrich RJ. Does hyperbaric oxygen therapy work in facilitating acute wound healing: a systematic review. Plast Reconstr Surg 2014;133:208e-215e. https://doi.org/10.1097/01.prs.0000436849.79161.a4
  2. Tibbles PM, Edelsberg JS. Hyperbaric-oxygen therapy. N Engl J Med 1996;334:1642-8. https://doi.org/10.1056/NEJM199606203342506
  3. Howard MA, Asmis R, Evans KK, Mustoe TA. Oxygen and wound care: a review of current therapeutic modalities and future direction. Wound Repair Regen 2013;21:503-11. https://doi.org/10.1111/wrr.12069
  4. Hopf HW, Gibson JJ, Angeles AP, Constant JS, Feng JJ, Rollins MD, et al. Hyperoxia and angiogenesis. Wound Repair Regen 2005;13:558-64. https://doi.org/10.1111/j.1524-475X.2005.00078.x
  5. Jan AM, Sandor GK, Iera D, Mhawi A, Peel S, Evans AW, et al. Hyperbaric oxygen results in an increase in rabbit calvarial critical sized defects. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006;101:144-9. https://doi.org/10.1016/j.tripleo.2005.08.032
  6. Jan A, Sandor GK, Brkovic BB, Peel S, Evans AW, Clokie CM. Effect of hyperbaric oxygen on grafted and nongrafted calvarial critical-sized defects. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2009;107:157-63. https://doi.org/10.1016/j.tripleo.2008.07.010
  7. Pedersen TO, Xing Z, Finne-Wistrand A, Hellem S, Mustafa K. Hyperbaric oxygen stimulates vascularization and bone formation in rat calvarial defects. Int J Oral Maxillofac Surg 2013;42:907-14. https://doi.org/10.1016/j.ijom.2013.01.003
  8. Chang H, Oh SE, Oh S, Hu KS, Kim S. Four-week histologic evaluation of grafted calvarial defects with adjunctive hyperbaric oxygen therapy in rats. J Periodontal Implant Sci 2016;46:244-53. https://doi.org/10.5051/jpis.2016.46.4.244
  9. Laurencin C, Khan Y, El-Amin SF. Bone graft substitutes. Expert Rev Med Devices 2006;3:49-57. https://doi.org/10.1586/17434440.3.1.49
  10. Palti A, Hoch T. A concept for the treatment of various dental bone defects. Implant Dent 2002;11:73-8. https://doi.org/10.1097/00008505-200201000-00017
  11. Kim S, Jung UW, Lee YK, Choi SH. Effects of biphasic calcium phosphate bone substitute on circumferential bone defects around dental implants in dogs. Int J Oral Maxillofac Implants 2011;26:265-73.
  12. Choi H, Park NJ, Jamiyandorj O, Choi KH, Hong MH, Oh S, et al. Improvement of osteogenic potential of biphasic calcium phosphate bone substitute coated with two concentrations of expressed recombinant human bone morphogenetic protein 2. J Periodontal Implant Sci 2012;42:119-26. https://doi.org/10.5051/jpis.2012.42.4.119
  13. Shin YS, Seo JY, Oh SH, Kim JH, Kim ST, Park YB, et al. The effects of ErhBMP-2-/EGCG-coated BCP bone substitute on dehiscence around dental implants in dogs. Oral Dis 2014;20:281-7. https://doi.org/10.1111/odi.12109
  14. Wong DA, Kumar A, Jatana S, Ghiselli G, Wong K. Neurologic impairment from ectopic bone in the lumbar canal: a potential complication of off-label PLIF/TLIF use of bone morphogenetic protein-2 (BMP-2). Spine J 2008;8:1011-8. https://doi.org/10.1016/j.spinee.2007.06.014
  15. Kaneko H, Arakawa T, Mano H, Kaneda T, Ogasawara A, Nakagawa M, et al. Direct stimulation of osteoclastic bone resorption by bone morphogenetic protein (BMP)-2 and expression of BMP receptors in mature osteoclasts. Bone 2000;27:479-86. https://doi.org/10.1016/S8756-3282(00)00358-6
  16. Smucker JD, Rhee JM, Singh K, Yoon ST, Heller JG. Increased swelling complications associated with off-label usage of rhBMP-2 in the anterior cervical spine. Spine 2006;31:2813-9. https://doi.org/10.1097/01.brs.0000245863.52371.c2
  17. Jin P, Wu H, Xu G, Zheng L, Zhao J. Epigallocatechin-3-gallate (EGCG) as a pro-osteogenic agent to enhance osteogenic differentiation of mesenchymal stem cells from human bone marrow: an in vitro study. Cell Tissue Res 2014;356:381-90. https://doi.org/10.1007/s00441-014-1797-9
  18. Oka Y, Iwai S, Amano H, Irie Y, Yatomi K, Ryu K, et al. Tea polyphenols inhibit rat osteoclast formation and differentiation. J Pharmacol Sci 2012;118:55-64. https://doi.org/10.1254/jphs.11082FP
  19. Xiao SJ, Textor M, Spencer ND, Sigrist H. Covalent attachment of cell-adhesive, (Arg-Gly-Asp)-containing peptides to titanium surfaces. Langmuir 1998;14:5507-16. https://doi.org/10.1021/la980257z
  20. Durrieu MC, Pallu S, Guillemot F, Bareille R, Amedee J, Baquey CH, et al. Grafting RGD containing peptides onto hydroxyapatite to promote osteoblastic cells adhesion. J Mater Sci Mater Med 2004;15:779-86. https://doi.org/10.1023/B:JMSM.0000032818.09569.d9
  21. Jan A, Sandor GK, Brkovic BB, Peel S, Kim YD, Xiao WZ, et al. Effect of hyperbaric oxygen on demineralized bone matrix and biphasic calcium phosphate bone substitutes. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2010;109:59-66. https://doi.org/10.1016/j.tripleo.2009.07.036
  22. Sirin Y, Olgac V, Dogru-Abbasoglu S, Tapul L, Aktas S, Soley S. The influence of hyperbaric oxygen treatment on the healing of experimental defects filled with different bone graft substitutes. Int J Med Sci 2011;8:114-25. https://doi.org/10.7150/ijms.8.114
  23. Develioglu H, Saraydin SU, Bolayir G, Dupoirieux L. Assessment of the effect of a biphasic ceramic on bone response in a rat calvarial defect model. J Biomed Mater Res A 2006;77A:627-31. https://doi.org/10.1002/jbm.a.30692
  24. Sawai T, Niimi A, Takahashi H, Ueda M. Histologic study of the effect of hyperbaric oxygen therapy on autogenous free bone grafts. J Oral Maxillofac Surg 1996;54:975-81. https://doi.org/10.1016/S0278-2391(96)90396-1
  25. Fok TC, Jan A, Peel SA, Evans AW, Clokie CM, Sandor GK. Hyperbaric oxygen results in increased vascular endothelial growth factor (VEGF) protein expression in rabbit calvarial critical-sized defects. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2008;105:417-22. https://doi.org/10.1016/j.tripleo.2007.07.015
  26. Gosain AK, Song L, Yu P, Mehrara BJ, Maeda CY, Gold LI, et al. Osteogenesis in cranial defects: reassessment of the concept of critical size and the expression of TGF-beta isoforms. Plast Reconstr Surg 2000;106:360-71. https://doi.org/10.1097/00006534-200008000-00018
  27. Cooper GM, Mooney MP, Gosain AK, Campbell PG, Losee JE, Huard J. Testing the critical size in calvarial bone defects: revisiting the concept of a critical-size defect. Plast Reconstr Surg 2010;125:1685-92. https://doi.org/10.1097/PRS.0b013e3181cb63a3
  28. Danmark S, Finne-Wistrand A, Wendel M, Arvidson K, Albertsson AC, Mustafa K. Osteogenic differentiation by rat bone marrow stromal cells on customized biodegradable polymer scaffolds. J Bioact Compat Polym 2010;25:207-23. https://doi.org/10.1177/0883911509358812
  29. Mardas N, Kostopoulos L, Karring T. Bone and suture regeneration in calvarial defects by e-PTFE-membranes and demineralized bone matrix and the impact on calvarial growth: an experimental study in the rat. J Craniofac Surg 2002;13:453-62. https://doi.org/10.1097/00001665-200205000-00017
  30. Humber CC, Sandor GK, Davis JM, Peel SA, Brkovic BM, Kim YD, et al. Bone healing with an in situ-formed bioresorbable polyethylene glycol hydrogel membrane in rabbit calvarial defects. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2010;109:372-84. https://doi.org/10.1016/j.tripleo.2009.10.008