한국식품과학회지 (Korean Journal of Food Science and Technology)

한국식품과학회 (Korean Society of Food Science and Technology)
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국내산 및 중국산 들깨 종자의 대사체 분석

Metabolomic analysis of perilla seeds harvested from Korea and China

  • 구수연 (경상대학교 응용생명과학부(BK21 plus)) ;
  • 최나영 (경상대학교 응용생명과학부(BK21 plus)) ;
  • 손예진 (경상대학교 응용생명과학부(BK21 plus)) ;
  • 박지영 (경상대학교 응용생명과학부(BK21 plus)) ;
  • 최성길 (경상대학교 농업생명과학대학 식품공학과) ;
  • 이명희 (국립식량과학원 남부작물부) ;
  • 김현진 (경상대학교 응용생명과학부(BK21 plus))
  • Gu, Suyeon (Division of Applied Life Sciences (BK21 plus), Gyeongsang National University) ;
  • Choi, Nayoung (Division of Applied Life Sciences (BK21 plus), Gyeongsang National University) ;
  • Son, Yejin (Division of Applied Life Sciences (BK21 plus), Gyeongsang National University) ;
  • Park, Ji Yeong (Division of Applied Life Sciences (BK21 plus), Gyeongsang National University) ;
  • Choi, Sung-Gil (Department of Food Science and Technology, and Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Lee, Myoung Hee (National Institute of Crop Science, Rural Development Administration) ;
  • Kim, Hyun-Jin (Division of Applied Life Sciences (BK21 plus), Gyeongsang National University)
  • 투고 : 2019.06.28
  • 심사 : 2019.10.09
  • 발행 : 2019.10.31
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초록

국내산 및 중국산 들깨 종자에 들어있는 지방산과 이차대사물질들의 프로파일들을 대사체 분석 기술을 이용하여 종합적으로 비교한 결과 PLS-DA score plot 상에서 국내산 들샘, 다미, 소담들깨 종자들과 중국산 들깨 종자들과 서로 분리되는 것을 확인하였다. 들깨 종자들 사이의 차이에 관여하는 물질들을 분석한 결과 지방산, phytosterols, 페놀화합물을 포함하는 23종의 물질들이 관여하는 것으로 확인하였다. 이들의 함량과 재배환경의 상관관계를 분석한 결과, 강수량, 온도, 위도, 고도가 들깨 종자 대사물질의 변화에 영향을 준 것으로 확인하였다. 동일 품종을 사용한 다양한 재배환경의 변화가 들깨 종자의 품질뿐만 아니라 들깨의 생육에 미치는 연구가 더 필요함에도 불구하고 본 연구결과는 재배환경이 다른 지역에서 생산된 들깨 종자를 구별할 수 있는 기초자료로 활용될 수 있을 것으로 사료된다.

A comprehensive comparison of fatty acid and metabolite profiles in 8 species of perilla seeds harvested from Korea and China was carried out to understand the correlation between cultivation conditions and the quality of perilla seeds. Metabolomic studies revealed that the perilla seeds were distinct from each other, based on the partial least squares -discriminant analysis (PLS-DA) score plots; 23 compounds including metabolites, fatty acids, and phytosterols that predominantly contributed to this interspecies difference were identified. Changes in the growth environment affected these compounds. In particular, phenolic compounds were affected by rainfall and temperature. Moreover, temperature and altitude showed negative and positive correlations, respectively, to oleic acid. Although more studies on the effect of various environmental factors on cultivation of perilla plants as well as their seeds are needed, the present results provide information that would be useful in identifying perilla seeds produced in specific regions and in the cultivation of good quality perilla.

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