Journal of Environmental Health Sciences (한국환경보건학회지)

Korean Society of Environmental Health (한국환경보건학회)
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Preventive Effect of Garlic Administration on Respiratory Toxicity Induced through Intratracheal Instillation of Fine Dust (PM10) in Rats

실험동물 랫드를 이용한 미세먼지 기도노출에 따른 호흡기계 독성에 대한 마늘의 예방효과 탐색

  • Lee, YoonBum (Department of Toxicity Assessment, Graduate School of Medical and Health Industry, Daegu Catholic University) ;
  • Kim, GeunWoo (Department of Toxicity Assessment, Graduate School of Medical and Health Industry, Daegu Catholic University) ;
  • Song, YoungMin (Department of Toxicity Assessment, Graduate School of Medical and Health Industry, Daegu Catholic University) ;
  • Han, YoungHoon (Department of Toxicity Assessment, Graduate School of Medical and Health Industry, Daegu Catholic University) ;
  • Ha, ChangSu (Department of Toxicity Assessment, Graduate School of Medical and Health Industry, Daegu Catholic University) ;
  • Lee, JiSun (GLP Center, Daegu Catholic University) ;
  • Kim, MinHee (GLP Center, Daegu Catholic University) ;
  • Son, HyeYoung (GLP Center, Daegu Catholic University) ;
  • Lee, GiYong (GLP Center, Daegu Catholic University) ;
  • Heo, Yong (Department of Toxicity Assessment, Graduate School of Medical and Health Industry, Daegu Catholic University) ;
  • Kim, ChangYul (Department of Toxicity Assessment, Graduate School of Medical and Health Industry, Daegu Catholic University)
  • 이윤범 (대구가톨릭대학교 의료보건산업대학원 화학물질독성평가학과) ;
  • 김근우 (대구가톨릭대학교 의료보건산업대학원 화학물질독성평가학과) ;
  • 송영민 (대구가톨릭대학교 의료보건산업대학원 화학물질독성평가학과) ;
  • 한영훈 (대구가톨릭대학교 의료보건산업대학원 화학물질독성평가학과) ;
  • 하창수 (대구가톨릭대학교 의료보건산업대학원 화학물질독성평가학과) ;
  • 이지선 (대구가톨릭대학교 GLP센터) ;
  • 김민희 (대구가톨릭대학교 GLP센터) ;
  • 손혜영 (대구가톨릭대학교 GLP센터) ;
  • 이기용 (대구가톨릭대학교 GLP센터) ;
  • 허용 (대구가톨릭대학교 의료보건산업대학원 화학물질독성평가학과) ;
  • 김창열 (대구가톨릭대학교 의료보건산업대학원 화학물질독성평가학과)
  • Received : 2020.10.26
  • Accepted : 2020.11.16
  • Published : 2020.12.31
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Abstract

Objectives: Exposure to fine dust (PM10) could contribute to the occurrence of cardiovascular disease or respiratory abnormalities. Since garlic is known to possess an anti-oxidative stress effect, the present study was performed to evaluate the effect of garlic intake on fine dust-mediated pulmonary toxicity. Methods: Rats were intratracheally instilled with fine dust at 15 mg/kg body weight (BW)/day for five days following five-day intragastric intubation of garlic at 0.7 or 1.4 g/kgBW/day, or 13.1 mg/kgBW/day S-allyl-cysteine (SAC) as a reference component in garlic. Blood and bronchoalveolar lavage fluid (BALF) were collected. Results: Deposit of fine dust was visually and histopathologically observed in the lungs. Body weight gain during the instillation period was significantly lowered in all the groups instilled with fine dust. Neutrophil numbers in blood were significantly elevated in the fine dust alone group, but this alteration was diminished in the groups administered with garlic. Levels of serum glutathione were lower in the rats instilled with fine dust alone, and this decrease in the glutathione level seems dose-dependently compensated among the groups administered with garlic. Similar findings were observed in the BALF with statistical significance. Typical pulmonary histopathological observation related with inflammation was demonstrated in the lungs of the rats exposed to fine dust alone, whereas such histopathologic findings were not improved in the groups administered with garlic. Conclusion: The present study suggests that garlic intake could alleviate fine dust-mediated pulmonary or systemic toxicities. Further investigation is necessary to delineate the mechanism of garlic-mediated effects on pulmonary function.

Keywords

References

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