Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
권호 표지
DOI QR코드

DOI QR Code

Anti-allergic Effect of Graviola Leaf Extract in Human Mast Cells

인간 비만세포에서 그라비올라 잎 추출물의 항알러지 효과

  • 김대용 (우석대학교 한의과대학)
  • Received : 2020.11.30
  • Accepted : 2020.12.30
  • Published : 2020.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

This study is aimed at determining whether graviola (Annona muricata) beneficially influences immunoglobulin (Ig) E-mediated allergic reactions in human mast cells (LUVA cells). To examine the anti-allergic effect of graviola leaf extract (GLE), we treated antigen/IgE sensitized mast cells with extracts of various concentrations. In this study, the results of the in vitro model of IgE-mediated mast cell degranulation showed that GLE significantly inhibited the release of histamine, β-hexosaminidase, TNF-α, IL-4 and IL-6 in LUVA cells. Pretreatment with GLE suppressed the phosphorylation of antigen-induced Lyn and Syk, thus suppressing the downstream MAPKs pathways. The above results indicate GLE could suppress mast cell activation and allergic responses. Accordingly, it can be supported that GLE has the potential to be used as functional cosmetic material.

본 연구는 그라비올라(Annona muricata)가 인간 비만세포(LUVA 세포)에서 immunoglobulin(Ig) E-매개 알러지 반응에 어떠한 효과를 나타내는지 확인하는데 목적이 있다. 그라비올라 잎 추출물(GLE)의 항알러지 효과를 알아보기 위해 IgE로 감작된 비만세포에 다양한 농도의 추출물을 처리하였다. 비만세포의 IgE-매개 탈과립 실험 결과, GLE가 LUVA 세포에서 histamine, β-hexosaminidase, TNF-α, IL-4 및 IL-6의 방출을 유의하게 억제하였다. 또한 GLE는 IgE 유도에 의한 Lyn과 Syk의 인산화를 억제하였고 그 하위 경로에 있는 MAPK의 활성을 억제하였다. 이상의 결과는 GLE가 비만세포 활성화 및 알러지 반응을 억제할 수 있음을 보여주고, 이는 GLE가 항알러지 효과를 통하여 기능성 화장품 소재로서의 활용이 가능함을 시사한다.

Keywords

References

  1. J. D. Kim, D. K. Kim, H. S. Kim, A. R. Kim, B. Kim, E. Her, K. H. Park, H. S. Kim, Y. M. Kim, W. S. Choi, "Morus bombycis Extract Suppresses Mast Cell Activation and IgE-mediated Allergic Reaction in Mice", Journal of Ethnopharmacology, Vol.146, No.1, pp. 287-293, (2013). https://doi.org/10.1016/j.jep.2012.12.046
  2. M. Sun, Y. Wang, H. Zhao, W. Yao, X. Yu, "Anti-allergic Action of Bacillus Calmette-Guerin Extract in Experimental Mast cell-mediated Anaphylactic Models", Molecular Medicine Reports, Vol.16, No.5, pp. 6248-6254, (2017). https://doi.org/10.3892/mmr.2017.7383
  3. J. M. Kim, Y. K. Shin, B. O. Kim, J. K. Kim, S. H. Lee, Y. S. Kim, "Effect of Artemisia capillaris Extracts on Antioxidant Activity and Allergic Dermatitis", Journal of Life Science, Vol.22, No.7, pp. 958-963, (2012). https://doi.org/10.5352/JLS.2012.22.7.958
  4. H. Miyauchi-Hashimoto, H. Okamoto, A. Sugihara, T. Horio, "Therapeutic and prophylactic effects of PUVA photochemotherapy on atopic dermatitis-like lesions in NC/Nga mice", Photodermatology, Photoimmunology & Photomedicine, Vol.21, No.3, pp. 125-130, (2005). https://doi.org/10.1111/j.1600-0781.2005.00153.x
  5. W. M. Suh, S. B. Park, S. Lee, H. H. Kim, K. Suk, J. H. Son, T. K. Kwon, H. G. Choi, S. H. Lee, S. H. Kim, "Suppression of Mast-cell-mediated Allergic Inflammation by Lindera obtusiloba", Experimental Biology and Medicine, Vol.236, No.2, pp. 240-246, (2011). https://doi.org/10.1258/ebm.2010.010274
  6. H. J. Do, T. W. Oh, K. I. Park, "Ethanol Extract of Sesamum indicum Linn. Inhibits FcepsilonRI-Mediated Allergic Reaction via Regulation of Lyn/Syk and Fyn Signaling Pathways in Rat Basophilic Leukemic RBL-2H3 Mast Cells", Mediators of Inflammation, Vol.2019 pp. 5914396, (2019).
  7. H. H. Kim, J. S. Yoo, T. Y. Shin, S. H. Kim, "Aqueous Extract of Mosla chinensis Inhibits Mast cell-mediated Allergic Inflammation", The American Journal of Chinese Medicine, Vol.40, No.6, pp. 1257-1270, (2012). https://doi.org/10.1142/S0192415X12500930
  8. S. Fu, S. Ni, D. Wang, M. Fu, T. Hong, "Berberine Suppresses Mast cell-mediated Allergic Responses via Regulating FceRI-mediated and MAPK Signaling", International Immunopharmacology, Vol.71, pp. 1-6, (2019). https://doi.org/10.1016/j.intimp.2019.02.041
  9. S. Z. Moghadamtousi, M. Fadaeinasab, S. Nikzad, G. Mohan, H. M. Ali, H. A. Kadir, "Annona muricata (Annonaceae): A Review of Its Traditional Uses, Isolated Acetogenins and Biological Activities", International Journal of Molecular Sciences, Vol.16, No.7, pp. 15625-15658, (2015). https://doi.org/10.3390/ijms160715625
  10. A. I. Yajid, H. S. Ab Rahman, M. P. K. Wong, W. Z. Wan Zain, "Potential Benefits of Annona muricata in Combating Cancer: A Review", Malays Journal of Medical Sciences, Vol.25, No.1, pp. 5-15, (2018). https://doi.org/10.21315/mjms2018.25.1.2
  11. G. T. Kim, N. K. Tran, E. H. Choi, Y. J. Song, J. H. Song, S. M. Shim, T. S. Park, "Immunomodulatory Efficacy of Standardized Annona muricata (Graviola) Leaf Extract via Activation of Mitogen-Activated Protein Kinase Pathways in RAW 264.7 Macrophages", Evidence-Based Complementary and Alternative Medicine, Vol.2016 Artivle ID 2905127, (2016).
  12. Y. Gavamukulya, F. Wamunyokoli, H. A. El-Shemy, "Annona muricata: Is the Natural Therapy to Most Disease Conditions Including Cancer Growing in Our Backyard? A Systematic Review of Its Research History and Future Prospects", Asian Pacific Journal of Tropical Medicine, Vol.10, No.9, pp. 835-848, (2017). https://doi.org/10.1016/j.apjtm.2017.08.009
  13. A. P. Balderrama-Carmona, N. P. Silva-Beltran, J. C. Galvez-Ruiz, S. Ruiz-Cruz, C. Chaidez-Quiroz, E. F. Moran-Palacio, "Antiviral, Antioxidant, and Antihemolytic Effect of Annona muricata L. Leaves Extracts. Plants (Basel), Vol.9, No.12, E1650, (2020).
  14. M. J. Kim, Y. A. Choi, S. Lee, J. K. Choi, Y. Y. Kim, E. N. Kim, G. S. Jeong, T. Y. Shin, Y. H. Jang, S. H. Kim, "Prunus serrulata var. spontanea Inhibits Mast Cell Activation and Mast cell-mediated Anaphylaxis", Journal of Ethnopharmacology, Vol. 250, (2020).
  15. I. O. Ishola, O. Awodele, A. M. Olusayero, C. O. Ochieng, "Mechanisms of Analgesic and Anti-inflammatory Properties of Annona muricata Linn. (Annonaceae) Fruit Extract in Rodents", Journal of Medicinal Food, Vol.17, No.12, pp. 1375-1382, (2014). https://doi.org/10.1089/jmf.2013.0088
  16. T. Nakamura, N. Yoshida, Y. Yamanoi, A. Honryo, H. Tomita, H. Kuwabara, Y. Kojima, "Eucalyptus Oil Reduces Allergic Reactions and Suppresses Mast Cell Degranulation by Downregulating IgEFcepsilonRI Signalling", Scientific Reports, Vol.10, No.1, 20940, (2020). https://doi.org/10.1038/s41598-020-77039-5
  17. M. Shukry, A. M. El-Shehawi, W. M. El-Kholy, R. A. Elsisy, H. S. Hamoda, H. G. Tohamy, M. M. Abumandour, F. A. Farrag, "Ameliorative Effect of Graviola (Annona muricata) on Mono Sodium Glutamate-Induced Hepatic Injury in Rats: Antioxidant, Apoptotic, Anti-inflammatory, Lipogenesis Markers, and Histopathological Studies. Animals (Basel), Vol.10, No.11, 1996, (2020). https://doi.org/10.3390/ani10111996
  18. L. Li, G. Jin, J. Jiang, M. Zheng, Y. Jin, Z. Lin, G. Li, Y. Choi, G. Yan, "Cornuside Inhibits Mast cell-mediated Allergic Response by Down-regulating MAPK and NF-kappaB Signaling Pathways", Biochemical and Biophysical Research Communications, Vol. 473, No. 2, pp. 408-414, (2016). https://doi.org/10.1016/j.bbrc.2016.03.007