Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
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Risk assessment and evaluation of epidermal growth factor (EGF) transgenic soybean: responses of Cyprinus carpio fed on EGF transgenic soybean

  • Oh, Sung-Dug (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Min, Seok-Ki (Korea Testing & Research Institute) ;
  • Kim, Jae Kwang (Division of Life Sciences, College of Life Sciences and Bioengineering, Incheon National University) ;
  • Park, Jung-Ho (Bio-evaluation Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Chang-Gi (Bio-evaluation Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Park, Soo Yun (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration)
  • Received : 2020.09.09
  • Accepted : 2020.10.06
  • Published : 2020.12.01
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Abstract

The epidermal growth factor (EGF) transgenic soybean was developed and biosynthesis of human epidermal growth factor (hEGF) in soybean seeds was confirmed. Also, EGF transgenic soybean were found to contain a herbicide resistance selectable marker by introduction of phosphinothricin acetyltransferase (PAT) gene from the Streptomyces hygroscopicus. For biosafety assessment, the EGF transgenic soybean expressing the EGF biosynthesis gene EGF and herbicide resistant gene PAT was tested to determine effects on survival of Cyprinus carpio, commonly used as a model organism in ecotoxicological studies. C. carpio was fed 100% ground soybean suspension, EGF soybean or non-genetically modified (GM) counterpart soybean (Gwangan). Gene expression of EGF soybean was confirmed by PCR and ELISA to have EGF/PAT. Feeding test showed that no significant differences in cumulative immobility or abnormal response between C. carpio samples fed on EGF soybean and non-GM counterpart soybean. The 48 h-EC50 values of the EGF and non-GM soybean were 1,688 mg·L-1 (95% confidence limits: 1,585 - 1,798 mg·L-1) and 1,575 mg·L-1 (95% confidence limits: 1,433 - 1,731 mg·L-1), respectively. The soybean NOEC (no observed effect concentration) value for C. carpio was suggested to be 625 mg·L-1. We concluded that there was no significant difference in toxicity for non-target organisms (C. carpio) between the EGF soybean and non-GM counterparts.

Keywords

Acknowledgement

본 연구는 농촌진흥청 바이오그린21사업(과제번호: PJ01368603)의 지원에 의해 이루어진 것입니다.

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