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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Techniques for investigating mitochondrial gene expression

  • Park, Dongkeun (Department of Biological Sciences, School of Life Sciences, Ulsan National Institute of Science and Technology) ;
  • Lee, Soyeon (Department of Biological Sciences, School of Life Sciences, Ulsan National Institute of Science and Technology) ;
  • Min, Kyung-Tai (Department of Biological Sciences, School of Life Sciences, Ulsan National Institute of Science and Technology)
  • Received : 2019.09.25
  • Published : 2020.01.31
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Abstract

The mitochondrial genome encodes 13 proteins that are components of the oxidative phosphorylation system (OXPHOS), suggesting that precise regulation of these genes is crucial for maintaining OXPHOS functions, including ATP production, calcium buffering, cell signaling, ROS production, and apoptosis. Furthermore, heteroplasmy or mis-regulation of gene expression in mitochondria frequently is associated with human mitochondrial diseases. Thus, various approaches have been developed to investigate the roles of genes encoded by the mitochondrial genome. In this review, we will discuss a wide range of techniques available for investigating the mitochondrial genome, mitochondrial transcription, and mitochondrial translation, which provide a useful guide to understanding mitochondrial gene expression.

Keywords

References

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