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Effects of glucoraphanin in dexamethasone-induced skeletal muscle atrophy in vitro model

Dexamethasone으로 유도된 근위축 세포모델에서 glucoraphanin의 효과

  • Jeon, Sang Kyu (College of Korean Medicine, Dongguk University) ;
  • Kim, Ok Hyeon (College of Korean Medicine, Dongguk University) ;
  • Park, Su Mi (College of Korean Medicine, Dongguk University) ;
  • Lee, Ju-Hee (College of Korean Medicine, Dongguk University) ;
  • Park, Sun-Dong (College of Korean Medicine, Dongguk University)
  • Received : 2020.02.16
  • Accepted : 2020.02.22
  • Published : 2020.02.28

Abstract

Objectives : Glucoraphanin is one of the well-known natural glucosinolates found in cruciferous plants. In the present study, we investigated the effects and molecular mechanism of glucoraphanin in dexamethasone-induced skeletal muscle atrophy in vitro model. Methods : The cytotoxic effects of glucoraphanin on C2C12 myoblasts or myotubes were evaluated by MTT assay. The glucoraphanin was evaluated effects in dexamethasone-induced skeletal muscle atrophy in C2C12 myotubes using a real-time PCR, western blots analysis, and immunofluorescence staining of myosin heavy chain. Result : Glucoraphanin had no cytotoxicity on both C2C12 myoblasts or myotubes. Dexamethasone markedly induced muscle atrophy by up-regulating muscle-specific ubiquitin E3 ligase markers, atrogin-1 and MuRF1, and down-regulating MyoD, a myogenic regulatory factor whereas co-treatment of glucoraphanin and dexamethasone dose-dependently inhibited it. Furthermore, decreased expressions of p-Akt, p-FOXO1, and p-FOXO3a induced by dexamethasone were reversed by co-treatment with glucoraphanin and dexamethasone. In addition, dexamethasone obviously reduced myotube diameters, while co-treatment of glucoraphanin and dexamethasone increased those to a similar level as control. Conclusions : These results show that glucoraphanin suppresses dexamethasone-induced muscle atrophy in C2C12 myotubes through activation of Akt/FOXO signaling pathway.

Keywords

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