Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Antioxidant, anti-inflammatory, antibacterial and ovoprotective effects of mixture of Ulmi cortex and Smilacis rhizoma extracts

유백피, 토복령 추출물 혼합물의 항산화, 항염, 항균 및 난소세포 보호효과

  • Jeon, Sang Kyu (College of Korean Medicine, Dongguk University) ;
  • Ahn, Jung Yun (College of Korean Medicine, Dongguk University) ;
  • Park, Su Mi (College of Korean Medicine, Dongguk University) ;
  • Park, Sun-Dong (College of Korean Medicine, Dongguk University) ;
  • Lee, Ju-Hee (College of Korean Medicine, Dongguk University)
  • Received : 2020.02.13
  • Accepted : 2020.02.24
  • Published : 2020.02.28
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Abstract

Objectives : US extract is a mixture of each extract of Ulmi cortex and Smilacis rhizoma. In this study, we investigated the antioxidant, anti-inflammatory, antibacterial, and ovoprotective effects of US extract in in vitro model to identify potential candidates for improving female reproductive function. Methods : The antioxidant activity of US extract was measured using 1,1-diphenyl- 2-picrylhydrazyl free radical and superoxide anion radical scavenging assays. The anti-inflammatory effect of US extract on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells were determined with a nitric oxide (NO) assay, enzyme linked immunosorbent assays, and western blots analysis. The antibacterial activity of US extract against vaginitis infection microorganisms were determined with disc diffusion and minimum inhibitory concentration assays. The ovoprotective effect of US extract on 4-vinylcyclohexene diepoxide (VCD)-induced ovotoxicity in CHO-K1 cells were evaluated with a cell viability assay. Result : US extract showed good antioxidant capacity and inhibited LPS-induced NO production as well as iNOS and COX-2 expression and secretion of pro-inflammatory cytokine IL-6 without affecting the cell viability. It showed significant clear zones for Staphylococcus aureus and Candida albicans but did not indicate the clear zones for Escherichia coli and Enterococcus faecium. VCD-induced ovotoxicity in CHO-K1 cells was significantly reduced by US extract pre-treatment. Conclusions : These results demonstrate that US extract has antioxidant activity, anti-inflammatory effects on the LPS-stimulated macrophages, antibacterial activity against vaginitis infection microorganisms, and protective effects on the ovarian cells against VCD-induced ovotoxicity. These findings suggest that the US extract can be used as new prescriptions, supplements, functional foods, and cosmetics for improving female reproductive function.

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References

  1. Government of the Republic of Korea. The 3rd plan for ageing society and population (2016-2020). Sejong: Government of the Republic of Korea. 2015.
  2. Li Q, Geng X, Zheng W, Tang J, Xu B, Shi Q. Current understanding of ovarian aging. Sci China Life Sci 2012;55(8):659-69. https://doi.org/10.1007/s11427-012-4352-5
  3. Stoop D, Cobo A, Silber S. Fertility preservation for age-related fertility decline. Lancet 2014;384(9950):1311-9. https://doi.org/10.1016/S0140-6736(14)61261-7
  4. Merritt MA, Riboli E, Murphy N, Kadi M, Tjonneland A, Olsen A et al. Reproductive factors and risk of mortality in the European Prospective Investigation into Cancer and Nutrition; a cohort study. BMC Med 2015;13:252. https://doi.org/10.1186/s12916-015-0484-3
  5. Ministry of Health and Welfare (2012). Guide of family health services.
  6. Lee YR, Chu MS. Trends in reproductive health-related research on women in Korea: A systematic review of published studies since 1995*. J Korean Acad Soc Home Care Nurs 2015;22(2):237-245.
  7. Lee IC, Kim MK. Antioxidant, antimicrobial and anti-inflammatory of mixed medicinal herb extract. Kor J Herbol 2015;30(5):51-8. https://doi.org/10.6116/KJH.2015.30.5.51.
  8. Shin MK. Clinical Traditional Herbalogy. Seoul:Yeong Lim's Publisher. 2000:441-2,668-9.
  9. Korea institute of oriental medicine. Ulmi cortex B. Korean intellectual property office. 2007 Dec[Cited 2020 Feb 3]. Available from: https://doi.org/10.20929/KTKP.MED.0000079468
  10. Kim YH. Herbalogy. Seoul:Hanol Publisher 2014:154-7.
  11. Luderer U. Ovarian toxicity from reactive oxygen species. Vitam Horm 2014;94:99-127. https://doi.org/10.1016/B978-0-12-800095-3.00004-3
  12. Devine PJ, Perreault SD, Luderer U. Roles of reactive oxygen species and antioxidants in ovarian toxicity. Biol Reprod 2012;86(2):27.
  13. Kang JE, Kim HD, Park SY, Pan JG, Kim JH, Yum DY. Dietary supplementation with a Bacillus superoxide dismutase protects against ${\gamma}$-radiation-induced oxidative stress and ameliorates dextran sulphate sodium-induced ulcerative colitis in Mice. J Crohns Colitis 2018;12(7):860-9. https://doi.org/10.1093/ecco-jcc/jjy034
  14. Li YJ, Han Z, Ge L, Zhou CJ, Zhao YF, Wang DH, Ren J, Niu XX, Liang CG. C-phycocyanin protects against low fertility by inhibiting reactive oxygen species in aging mice. Oncotarget 2016;7(14):17393-409. https://doi.org/10.18632/oncotarget.8165
  15. Agarwal A, Gupta S, Sharma RK. Role of oxidative stress in female reproduction. Reprod Biol Endocrinol 2005;3:28. https://doi.org/10.1186/1477-7827-3-28
  16. Liu J, Liu M, Ye X, Liu K, Huang J, Wang L et al. Delay in oocyte aging in mice by the antioxidant N-acetyl-l-cysteine (NAC). Hum Reprod 2012; 27(5):1411-20. https://doi.org/10.1093/humrep/des019
  17. Liu M, Yin Y, Ye X, Zeng M, Zhao Q, Keefe DL et al. Resveratrol protects against age-associated infertility in mice. Hum Reprod 2013;28(3):707-17. https://doi.org/10.1093/humrep/des437
  18. Fortier AH. Activation of murine macrophages. Curr Protoc Immunol. 2001;Chapter 14:Unit 14.4.
  19. Cho SN. Updated treatment of vaginitis. Kor J Obstet Gynecol 2005;48(2):261-8.
  20. Paladine HL, Desai UA. Vaginitis: Diagnosis and Treatment. Am Fam Physician 2018;97(5):321-9.
  21. Bhattacharya P, Keating AF. Impact of environmental exposures on ovarian function and role of xenobiotic metabolism during ovotoxicity. Toxicol Appl Pharmacol 2012;261(3):227-35. https://doi.org/10.1016/j.taap.2012.04.009
  22. Sobinoff AP, Nixon B, Roman SD, McLaughlin EA. Staying alive: PI3K pathway promotes primordial follicle activation and survival in response to 3MC-induced ovotoxicity. Toxicol Sci. 2012;128(1):258-71. https://doi.org/10.1093/toxsci/kfs137
  23. Lambouras M, Liew SH, Horvay K, Abud HE, Stringer JM, Hutt KJ. Examination of the ovotoxicity of 5-fluorouracil in mice. J Assist Reprod Genet. 2018;35(6):1053-1060. https://doi.org/10.1007/s10815-018-1169-6
  24. Nam EY, Kim SA, Kim H, Kim SH, Han JH, Lee JH, Kim DI. Akt activation by Evodiae Fructus extract protects ovary against 4-vinylcyclohexene diepoxide-induced ovotoxicity. J Ethnopharmacol. 2016;194:733-9. https://doi.org/10.1016/j.jep.2016.10.048
  25. Hoyer PB, Devine PJ, Hu X, Thompson KE, Sipes IG. Ovarian toxicity of 4-vinylcyclohexene diepoxide: a mechanistic model. Toxicol Pathol. 2001;29(1):91-9. https://doi.org/10.1080/019262301301418892