Journal of Ginseng Research

  • 제44권3호
  • /
  • Pages.453-460
  • /
  • 2020
  • /
  • 1226-8453(pISSN)
  • /
  • 2093-4947(eISSN)
고려인삼학회 (The Korean Society of Ginseng)
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Panax ginseng-derived fraction BIOGF1K reduces atopic dermatitis responses via suppression of mitogen-activated protein kinase signaling pathway

  • Lorz, Laura Rojas (Department of Integrative Biotechnology, Sungkyunkwan University) ;
  • Kim, Donghyun (Heritage Material Research Team, Amorepacific R&D Unit) ;
  • Kim, Mi-Yeon (School of Systems Biomedical Science, Soongsil University) ;
  • Cho, Jae Youl (Department of Integrative Biotechnology, Sungkyunkwan University)
  • 투고 : 2018.12.31
  • 심사 : 2019.02.18
  • 발행 : 2020.05.15
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초록

Background: BIOGF1K, a fraction of Panax ginseng, has desirable antimelanogenic, anti-inflammatory, and antiphotoaging properties that could be useful for treating skin conditions. Because its potential positive effects on allergic reactions in skin have not yet been described in detail, this study's main objective was to determine its efficacy in the treatment of atopic dermatitis (AD). Methods: High-performance liquid chromatography was used to verify the compounds in BIOGF1K, and we used the (3-4-5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide method to determine its cytotoxicity in RBL-2H3 and HMC-1 cell lines. RBL-2H3 cells were induced using both anti-DNP-IgE/DNP-BSA and calcium ionophore (A2187) treatments, whereas HMC-1 cells were induced using A2187 alone. To measure mast cell degranulation, we performed histamine (enzyme-linked immunosorbent assay) and β-hexosaminidase assays. To quantify interleukin (IL)-4, IL-5, and IL-13 levels in RBL-2H3 cells, we performed quantitative polymerase chain reaction (PCR); to quantify expression levels of IL-4 and IL-13 in HMC-1 cells, we used semiquantitative reverse transcription polymerase chain reaction (RT-PCR). Finally, we detected the total and phosphorylated forms of extracellular signal-regulated kinase, p-38, and c-Jun N-terminal kinase proteins by immunoblotting. Results: BIOGF1K decreased the AD response by reducing both histamine and β-hexosaminidase release as well as reducing the secretion levels of IL-4, IL-5, and IL-13 in RBL-2H3 cells and IL-4 and IL-13 in HMC-1 cells. In addition, BIOGF1K decreased MAPK pathway activation in RBL-2H3 and HMC-1 cells. Conclusions: BIOGF1K attenuated the AD response, hence supporting its use as a promising and natural approach for treating AD.

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참고문헌

  1. Odhiambo JA, Williams HC, Clayton TO, Robertson CF, Asher MI. Global variations in prevalence of eczema symptoms in children from ISAAC Phase Three. J Allergy Clin Immunol 2009;124:1251-8. e1223. https://doi.org/10.1016/j.jaci.2009.10.009
  2. Lee JH, Son SW, Cho SH. A Comprehensive review of the treatment of atopic eczema. Allergy Asthma Immunol Res 2016;8:181-90. https://doi.org/10.4168/aair.2016.8.3.181
  3. Gandhi NA, Pirozzi G, Graham NMH. Commonality of the IL-4/IL-13 pathway in atopic diseases. Expert Rev Clin Immunol 2017;13:425-37. https://doi.org/10.1080/1744666X.2017.1298443
  4. Thomsen SF. Atopic dermatitis: natural history, diagnosis, and treatment. ISRN Allergy 2014;2014. 354250-354250. https://doi.org/10.1155/2014/354250
  5. Brandt EB, Sivaprasad U. Th2 cytokines and atopic dermatitis. J Clin Cell Immunol 2011;2:110.
  6. Oldhoff JM, Darsow U, Werfel T, Katzer K, Wulf A, Laifaoui J, Hijnen DJ, Plotz S, Knol EF, Kapp A, et al. Anti-IL-5 recombinant humanized monoclonal antibody (Mepolizumab) for the treatment of atopic dermatitis. Allergy 2005;60:693-6. https://doi.org/10.1111/j.1398-9995.2005.00791.x
  7. Brunner PM, Guttman-Yassky E, Leung DYM. The immunology of atopic dermatitis and its reversibility with broad-spectrum and targeted therapies. J Allergy Clin Immunol 2017;139:S65-76. https://doi.org/10.1016/j.jaci.2017.01.011
  8. Furue M, Yamamura K, Kido-Nakahara M, Nakahara T, Fukui Y. Emerging role of interleukin-31 and interleukin-31 receptor in pruritus in atopic dermatitis. Allergy 2018;73:29-36. https://doi.org/10.1111/all.13239
  9. De Benedetto A, Agnihothri R, McGirt LY, Bankova LG, Beck LA. Atopic dermatitis: a disease caused by innate immune defects? J Investig Dermatol 2009;129:14-30. https://doi.org/10.1038/jid.2008.259
  10. Ogi K, Takabayashi T, Yamada T, Sakashita M, Kanno M, Narita N, Fujieda S. Trimellitic anhydride induces low-grade mast cell degranulation without specific IgE. Toxicol Rep 2016;3:701-7. https://doi.org/10.1016/j.toxrep.2016.09.001
  11. Matsuda H, Nakamura S, Yoshikawa M. Degranulation inhibitors from medicinal plants in antigen-stimulated rat basophilic leukemia (RBL-2H3) cells. Chem Pharm Bull 2016;64:96-103. https://doi.org/10.1248/cpb.c15-00781
  12. Bansode RR, Plundrich NJ, Randolph PD, Lila MA, Williams LL. Peanut flour aggregation with polyphenolic extracts derived from peanut skin inhibits IgE binding capacity and attenuates RBL-2H3 cells degranulation via MAPK signaling pathway. Food Chem 2018;263:307-14. https://doi.org/10.1016/j.foodchem.2018.05.007
  13. Broeders JA, Ahmed Ali U, Fischer G. Systematic review and meta-analysis of randomized clinical trials (RCTs) comparing topical calcineurin inhibitors with topical corticosteroids for atopic dermatitis: a 15-year experience. J Am Acad Dermatol 2016;75:410-9. e413. https://doi.org/10.1016/j.jaad.2016.02.1228
  14. Hajar T, Leshem YA, Hanifin JM, Nedorost ST, Lio PA, Paller AS, Block J, Simpson EL. A systematic review of topical corticosteroid withdrawal (steroid addiction) in patients with atopic dermatitis and other dermatoses. J Am Acad Dermatol 2015;72:541-9. e542. https://doi.org/10.1016/j.jaad.2014.11.024
  15. Cury Martins J, Martins C, Aoki V, Gois AFT, Ishii HA, da Silva EMK. Topical tacrolimus for atopic dermatitis. Cochrane DB Syst Rev; 2015.
  16. Park HJ, Kim DH, Park SJ, Kim JM, Ryu JH. Ginseng in traditional herbal prescriptions. J Ginseng Res 2012;36:225-41. https://doi.org/10.5142/jgr.2012.36.3.225
  17. Kimura Y, Sumiyoshi M, Sakanaka M. Effects of ginsenoside $Rb_{1}on$ skin changes. J Biomed Biotechnol 2012;2012. 946242-946242.
  18. Kim EH, Kim W. An Insight into ginsenoside metabolite compound K as a potential tool for skin disorder. Evid-based Comp Alternat Med 2018. https://doi.org/10.1155/2018/8075870.8075870.
  19. Hossen M, Hong yd, Baek K-S, Yoo S, Hong Y, Hye Kim J, Lee J-O, Kim D, Park J, Cho J. In vitro anti-oxidative and anti-inflammatory effects of BIOGF1K, compound K-rich fraction, prepared fromPanax ginseng. J Ginseng Res 2017;41:43-51. https://doi.org/10.1016/j.jgr.2015.12.009
  20. Hong YH, Kim D, Nam G, Yoo S, Han SY, Jeong S-G, Kim E, Jeong D, Yoon K, Kim S, et al. Photoaging protective effects of BIOGF1K, a compound-K-rich fraction prepared from Panax ginseng. J Ginseng Res 2018;42:81-9. https://doi.org/10.1016/j.jgr.2017.01.002
  21. Sun Z, Zuo L, Sun T, Tang J, Ding D, Zhou L, Kang J, Zhang X. Chemical profiling and quantification of XueBiJing injection, a systematic quality control strategy using UHPLC-Q Exactive hybrid quadrupole-orbitrap high-resolution mass spectrometry. Sci Rep 2017;7:16921. https://doi.org/10.1038/s41598-017-17170-y
  22. Baek K-S, Yi Y-S, Son Y-J, Yoo S, Sung NY, Kim Y, Hong S, Aravinthan A, Kim JH, Cho JY. In vitro and in vivo anti-inflammatory activities of Korean Red Ginseng-derived components. J Ginseng Res 2016;40:437-44. https://doi.org/10.1016/j.jgr.2016.08.003
  23. Ben-David A, Davidson CE. Estimation method for serial dilution experiments. J Microbl Meth 2014;107:214-21. https://doi.org/10.1016/j.mimet.2014.08.023
  24. Kim E, Kim D, Yoo S, Hong YH, Han SY, Jeong S, Jeong D, Kim JH, Cho JY, Park J. The skin protective effects of compound K, a metabolite of ginsenoside Rb1 from Panax ginseng. J Ginseng Res 2018;42:218-24. https://doi.org/10.1016/j.jgr.2017.03.007
  25. Kee J-Y, Hong S-H. Ginsenoside Rg3 suppresses mast cellemediated allergic inflammation via mitogen-activated protein kinase signaling pathway. J Ginseng Res 2019. in press.
  26. Ekoff M, Strasser A, Nilsson G. $Fc{\varepsilon}RI$Aggregation promotes survival of connective tissue-like mast cells but not mucosal-like mast cells. J Immunol 2007;178:4177. https://doi.org/10.4049/jimmunol.178.7.4177
  27. Han SY, Kim J, Kim E, Kim SH, Seo DB, Kim JH, Shin SS, Cho JY. AKT-targeted anti-inflammatory activity of Panax ginseng calyx ethanolic extract. J Ginseng Res 2018;42:496-503. https://doi.org/10.1016/j.jgr.2017.06.003
  28. Park JG, Yi YS, Hong YH, Yoo S, Han SY, Kim E, Jeong SG, Aravinthan A, Baik KS, Choi SY, et al. Tabetri (Tabebuia avellanedae ethanol extract) ameliorates osteoarthritis symptoms induced by monoiodoacetate through its antiinflammatory and chondroprotective activities. Mediators Inflamm 2017;2017:3619879.
  29. Lee JO, Kim E, Kim JH, Hong YH, Kim HG, Jeong D, Kim J, Kim SH, Park C, Seo DB, et al. Antimelanogenesis and skin-protective activities of Panax ginseng calyx ethanol extract. J Ginseng Res 2018;42:389-99. https://doi.org/10.1016/j.jgr.2018.02.007
  30. Lee KG, Son SW. Efficacy of Korean Red Ginseng in the treatment of atopic dermatitis. J Ginseng Res 2011;35:149-54. https://doi.org/10.5142/jgr.2011.35.2.149
  31. Kee J-Y, Jeon Y-D, Kim D-S, Han Y-H, Park J, Youn D-H, Kim S-J, Ahn KS, Um JY, Hong S-H. Korean Red Ginseng improves atopic dermatitis-like skin lesions by suppressing expression of proinflammatory cytokines and chemokines in vivo and in vitro. J Ginseng Res 2017;41:134-43. https://doi.org/10.1016/j.jgr.2016.02.003
  32. Kim JR, Choi J, Kim J, Kim H, Kang H, Kim EH, Chang J-H, Kim Y-E, Choi YJ, Lee KW, et al. 20-O-${\beta}$-d-glucopyranosyl-20(S)-protopanaxadiol-fortified ginseng extract attenuates the development of atopic dermatitis-like symptoms in NC/Nga mice. J Ethnopharmacol 2014;151:365-71. https://doi.org/10.1016/j.jep.2013.10.058
  33. Radinger M, Jensen BM, Kuehn HS, Kirshenbaum A, Gilfillan AM. Generation, isolation, and maintenance of human mast cells and mast cell lines derived from peripheral blood or cord blood. Curr Prot Immunol 2010. https://doi.org/10.1002/0471142735.im0737s90 [Chapter 7]:Unit-7.37.
  34. Kawai M, Hirano T, Higa S, Arimitsu J, Maruta M, Kuwahara Y, Ohkawara T, Hagihara K, Yamadori T, Shima Y, et al. Flavonoids and related compounds as anti-allergic substances. Allergol Int 2007;56:113-23. https://doi.org/10.2332/allergolint.R-06-135
  35. Lee B-H,Kim H-K, Jang M,Kim H-J, Choi S-H,HwangS-H,Kim H-C, Rhim H, Cho IH, Nah S-Y. Effects of gintonin-enriched fraction in an atopic dermatitis animal model: involvement of autotaxin regulation. Biol Pharm Bull 2017;40:1063-70. https://doi.org/10.1248/bpb.b17-00124
  36. Bae E-A, Han MJ, Shin Y-W, Kim D-H. Inhibitory effects of Korean Red Ginseng and its genuine constituents ginsenosides Rg3, Rf, and Rh2 in mouse passive cutaneous anaphylaxis reaction and contact dermatitis models. Biol Pharm Bull 2006;29:1862-7. https://doi.org/10.1248/bpb.29.1862

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