Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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Anti-inflammatory Effects of Haliotis discus-derived Antibacterial Peptide (AMP) Ab4-7 as a Functional material for Improving Inflammatory Skin Diseases

염증성 피부질환 개선 기능 소재로서 둥근전복(Haliotis discus) 유래 항균펩타이드 Ab4-7의 항염증 효과

  • Choi, Soo-Cheol (Department of Chemistry, College of Natural Science, Kunsan National University) ;
  • Seo, Jung-Kil (Department of Food Science and Biotechnology, College of Natural Science, Kunsan National University) ;
  • Hwang, Joon-Ho (Jeju Love Co. Ltd) ;
  • Lee, Ki-Young (Department of Marine Biotechnology, Kunsan National University) ;
  • Lee, In-Ah (Department of Chemistry, College of Natural Science, Kunsan National University)
  • 최수철 (군산대학교 자연과학대학 화학과) ;
  • 서정길 (군산대학교 해양과학대학 식품생명과학부) ;
  • 황준호 ((주)제주사랑농수산) ;
  • 이기영 (군산대학교 해양과학대학 해양생명응용과학부) ;
  • 이인아 (군산대학교 자연과학대학 화학과)
  • Received : 2020.04.08
  • Accepted : 2020.06.05
  • Published : 2020.06.30
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Abstract

Abalone, a marine organism inhabiting the west coast of Korea, has antioxidant and anti-inflammatory effects, and is a resource with potential to be used in various industries such as antibiotic development and cosmetic raw materials. In this study, we chose abalone among various marine lives on the west coast. Antibacterial peptide (AMP) was separated from abalone and its derivative Ab4-7 was identified and its physiological activity was studied. The treatment of Ab4-7 in inflammatory RAW 264.7 to check the anti-inflammatory efficacy nhibited inflammatory cytokines, TLR4, TNF-α, IL-1β, COX-2, and iNOS and increased mRNA manifestation of HO-1, genes related to antioxidants. Based on the strong antioxidant and anti-inflammatory effects of Ab4-7, the effects of Ab4-7 in the inflammatory RAW 264.7 cells were identified through RT-PCR, which regulates the gene Matrix Metalloproteinase (MMPs) that induces a variety of inflammatory skin diseases by engaging in the decomposition of the extrocellular matrix metalloproteinase (ECM). Taken together, it is concluded that Ab4-7 has a powerful anti-inflammatory and antioxidant effect and can be used as a functional material for various inflammatory skin disease treatments by controlling the genes associated with matrix metalloproteinase (MMPs).

서해안에 서식하는 해양생물인 전복은 항산화 및 항염증 효과가 있으며, 항생제 개발 및 화장품 원료 등 다양한 산업에 활용될 수 있는 잠재력을 가진 자원이다. 본 연구에서 우리는 서해안의 다양한 해양 생물 중에서 전복을 선택하였으며, 전복에서 분리한 항균펩타이드(AMP)를 이용하여 항균펩타이드 유도체인 Ab4-7를 규명하고 생리활성을 연구하였다. Ab4-7의 항염증 효능을 확인하기 위해 염증이 유도된 RAW 264.7에 Ab4-7을 처리한 결과, 염증성 사이토카인, TLR4, TNF-α, IL-1β, COX-2, iNOS 등을 억제하고 항산화와 관련된 유전인자인 HO-1의 mRNA 발현을 증가시켰다. Ab4-7의 강력한 항산화 및 항염증 효과를 바탕으로, 염증이 유도된 RAW 264.7 세포에서 Ab4-7이 extrocellular matrix metalloproteinase (ECM) 분해에 관여하여 다양한 염증성 피부질환을 유도하는 유전자인 matrix metalloproteinase (MMPs)를 조절하는 효과를 RT-PCR을 통해 확인하였다. Ab4-7은 강력한 항염증 및 항산화 효과를 가지고 있으며, 항염증 및 항산화 효과를 통한 MMPs 관련 유전인자 조절을 통해 다양한 염증성 피부질환 치료제의 기능성 재료로 활용될 수 있다고 사료된다.

Keywords

References

  1. S. H. Moon, E. J. Yang, B. B. Song, B. M. Kim, J. S. Lee, and I. S. Kim, Effects of benzene, phenol and hydroquinone on proliferation, differentiation and migration of human eosinophilic EoL-1 cells, J. Exp. Biomed. Sci, 16(3), 179 (2010).
  2. S. A. Kim, S. C. Choi, Y. H. Youn, C. I. Ko, Y. S. Ha, and I. A. Lee, Antioxidant and anti-inflammatory effects of Dioscorea Japonica and Chenopodium album, J. Soc. Cosmet. Sci. Korea, 43(4), 337 (2017 ). https://doi.org/10.15230/SCSK.2017.43.4.337
  3. M. L. McDaniel, G. Kwon, J. R. Hill, C. A. Marshall, and J. A. Corbett, Cytokines and nitric oxide in islet inflammation and diabetes, Proc. Soc. Exp. Biol Med., 211(1), 24 (1996). https://doi.org/10.3181/00379727-211-43950D
  4. S. M. An, H. G. Kim, E. J. Choi, H. H. Hwang, E. S. Lee, J. H. Beak, Y. C. Boo, and J. S. Koh, Screening for anti-inflammatory activities in extracts from Korean herb medicines, J. Soc. Cosmet. Sci. Korea, 40(1), 95 (2014). https://doi.org/10.15230/SCSK.2014.40.1.95
  5. E. G. Lee, B. M. Mickle-Kawar, and R. M. Gallucci, IL-6 deficiency exacerbates skin inflammation in a murine model of irritant dermatitis, J Immunotoxicol, 10(2), 192 (2013) https://doi.org/10.3109/1547691X.2012.707700
  6. M. Kamsteeg, P. A. Jansen, I. M. van VlijmenWillems, P. E. van Erp, D. Rodijk-Olthuis, P. G. van der Valk, T. Feuth, P. L. Zeeuwen, and J. Schalkwijk, Molecular diagnostics of psoriasis, atopic dermatitis, allergic contact dermatitis and irritant contact dermatitis, Br. J. Dermatol., 162(3), 568 (2010). https://doi.org/10.1111/j.1365-2133.2009.09547.x
  7. J. Seneschal, E. Kubica, L. Boursault, J. Stokkermans, C. Labreze, B. Milpied, K. Ezzedine, and A. Taieb, Exogenous inflammatory acne due to combined application of cosmetic and facial rubbing, Dermatology, 224(3), 221 (2012). https://doi.org/10.1159/000338694
  8. K. Aono, K. Isobe, K. Kiuchi, Z. H. Fan, M. Ito, A. Takeuchi, M. Miyachi, I. Nakashima, and Y. Nimura, In vitro and in vivo expression of inducible nitric oxide synthase during experimental endotoxemia: involvement of other cytokines, J. Cell. Biochem., 65(3), 349 (1997). https://doi.org/10.1002/(SICI)1097-4644(19970601)65:3<349::AID-JCB5>3.0.CO;2-S
  9. H. M. Lee, J. H. Choi, C. S. Choi, S. J. Hwang, and H. L. Lee, Expression of MMP-9 and TIMP-1 in the nasal mucosa of allergic rhinitis, Korean J Otolaryngol, 43(6), 604 (2000).
  10. J. T. Lee, K. J. Woo, and T. K. Kwon, Effect of sulforaphane on LPS-induced matrix metalloproteinase-9 (MMP-9) expression, Journal of Life Science, 20(2), 275 (2010). https://doi.org/10.5352/JLS.2010.20.2.275
  11. J. W. Lim, Y. J. Cho, D. H. Lee, B. C. Jung, H. S. Kang, T. J. Kim, K. J. Rhee, T. U. Kim, and Y. S. Kim, Upregulation of MMP is mediated by MEK1 activation during differentiation of monocyte into macrophage, J. Exp. Biomed. Sci, 18(2), 104 (2012).
  12. M. Steenport, K. M. Faisal Khan, B. Du, S. E. Barnhard, J. Andrew, and D. J. Falcone, Matrix metalloproteinase (MMP)-1 and MMP-3 induce macrophage MMP-9: Evidence for the role of TNF-${\alpha}$ and cyclooxygenase-2, J. Immunol., 183(12), 8119 (2009). https://doi.org/10.4049/jimmunol.0901925
  13. S. Ryan, A. G. Abdel, A. Nikola, and N. Salvador, Suppression of LPS-induced matrix-metalloproteinase responses in macrophages exposed to phenytoin and its metabolite, 5-(p-hydroxyphenyl-), 5-phenylhydantoin, Journal of Inflammation, 7(48), 48 (2010). https://doi.org/10.1186/1476-9255-7-48
  14. J. C. Fantone and P. A. Ward, Role of oxygen-derived free radicals and metabolites in leukocyte dependent inflammatory reaction, Am. J. Pathol, 107(3), 395 (1982).
  15. R. K. Oh, M. J. Lee, Y. O. Kim, B. H. Nam, H. J. Kong, J. W. Kim, C. M. An, and D. G. Kim, Isolation and purification of antimicrobial peptide from hard-shelled mussel, Mytilus coruscus, Journal of Life Science, 26(11), 1259 (2016). https://doi.org/10.5352/JLS.2016.26.11.1259
  16. J. K. Seo, Screening and purification of an antimicrobial peptide from the gill of the Manila clam ruditapes philippinarum, Korea. J. Fish Aquat Sci, 49(2), 137 (2016). https://doi.org/10.5657/KFAS.2016.0137
  17. C. S. You, J. H. Jung, and D. K. Lee, An effect of resveratrol on the formation of lipid membrane fragmentations caused by the interaction between antimicrobial peptide pexiganan (MSI-78) and lipid bilayer membranes, Biomater. Res, 17(4), 168 (2013).
  18. H. S. Kim, H. S. Jeong, J. Kim, A. Y. Yun, K. W. Lee, and S. H. Cho, Substitution effect of fish meal and saccharina with soybean meal and ricc bran in the extruded pellet on juvenile abalone Haliotis discus (Reeve 1846), Korean. J. Fish Aquat Sci, 50(6), 731 (2017). https://doi.org/10.5657/KFAS.2017.0731
  19. S. Y. Lim, Cytotoxic and antioxidant activities of abalone (Haliotis discus hannal) extracts, Extracts, Journal of Life Science, 24(7), 737 (2014). https://doi.org/10.5352/JLS.2014.24.7.737
  20. S. M. Lee, C. S. Park, and D. S. Kim, Effects of dietary herbs on growth and body composition of juvenile abalone, Haliotis discus hannai, Korean. J. Fish Aquat Sci. 34(5), 570 (2001).
  21. S. Y. Moon, H. S. Yoon, D. C. Seo, and S. D. Choi, Crowth comparison of juvenile abalone, in different culture systems in the west coast of Korea, J. Aquaculture, 19(4), 242 (2006).
  22. M. J. Yoo and H. J. Chung, Optimal manufacturing condition and quality properties of the drinking of disk abalone, Koran. J. Food Culture, 22(6), 827 (2007).
  23. J. K. Seo, M. J. Lee, B. H. Nam, and N. G. Park, cgMolluscidin, a novel dibasic residue repeat rich antimicrobial peptide, purified from the gill of the Pacific oyster, Crassostrea gigas, Fish Shellfish Immunol, 35(2), 480 (2013). https://doi.org/10.1016/j.fsi.2013.05.010
  24. J. K. Seo, H. J. Go, C. H. Kim, B. H. Nam, and N. G. Park, Antimicrobial peptide, hdMolluscidin, purified from the gill of the abalone, Haliotis discus, Fish Shellfish Immunol, 52, 289 (2016). https://doi.org/10.1016/j.fsi.2016.03.150
  25. B. H. Nam, J. Y. Moon, E. H. Park, Y. O. Kim, D. G. Kim, H. J. Kong, W. J. Kim, Y. J. Jee, C. M. An, N. G. Park, and J. K. Seo, Antimicrobial activity of peptides derived from olive flounder lipopolysaccharide binding protein/bactericidal permeability-increasing protein (LBP/BPI), Mar Drugs, 12(10), 5240 (2014). https://doi.org/10.3390/md12105240
  26. R. I. Lehrer, M. Rosenman, S. S. I. Harwig, R. Jackson, P. Eisenhaur, Ultrasensitive assay for endogenous antimicrobial polypeptides, J. Immunol Methods, 137(2), 167 (1991). https://doi.org/10.1016/0022-1759(91)90021-7
  27. World Mediccal Association Declaration of Helsinki: Ethical Principles for Medical Research Involving Human Subjects, JAMA : the journal of the American Medical Association, (2013).
  28. Korea Food & Drug Administration, Clinical test and in vitro test guideline for cosmetics (2011).
  29. P. J. Frosch and A. M. Kligman, The soap chamber test, A new method for assessing the irritancy of soaps, J. Am. Acad. Dermatol, 1(1) 35 (1979). https://doi.org/10.1016/s0190-9622(79)70001-6
  30. Y. Yamamoto, P. He, T. Klein, and H. Friedman, Endotoxin induced cytotoxicity of macrophages is due to apoptosis caused by nitric oxide production, J. Endotoxin Research, 1(3), 181 (1994). https://doi.org/10.1177/096805199400100307
  31. S. Y. Kim, Effects of long-term treadmill exercise on heme oxygenase-1 expression in mouse tissues, Exercise Sci, 19(1), 15 (2010). https://doi.org/10.15857/ksep.2010.19.1.15
  32. J. L. Adams and C. J. Czuprynski, Mycobacterial cell wall components induce the production of TNF-${\alpha}$, IL-1, and IL-6 by bovine monocytes and the murine macrophage cell line RAW 264.7, Microbial pathogenesis, 16(6), 401 (1994). https://doi.org/10.1006/mpat.1994.1040
  33. M. Iwamoto, M. Kurachi, T. Nakashima, D. Kim, K. Yamaguchi, T. Oda, Y. Iwamoto, and T. Muramatsu, Structure-activity relationship of alginate oligosaccharides in the induction of cytokine production from RAW264. 7 cells, FEBS lett, 579(20), 4423 (2005). https://doi.org/10.1016/j.febslet.2005.07.007