Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Effects of Exercise Type on ẞ-Amyloid, BDNF and Cognitive Function in Type 2 Diabetic Mice

제 2형 당뇨 마우스의 운동 형태가 ẞ-Amyloid, BDNF 및 인지기능에 미치는 영향

  • Kim, Do-Yeon (Department of Physical Education, Pusan National University) ;
  • Woo, Jinhee (Department of Physical Education, Dong-A University) ;
  • Shin, Ki-Ok (Department of Physical Education, Dong-A University) ;
  • Roh, Hee-Tae (Department of Physical Education, Dong-A University) ;
  • Lee, Yul-Hyo (Department of Physical Education, Dong-A University) ;
  • Yoon, Byung-Kon (Department of Physical Education, Dong-Eui University) ;
  • Park, Chan-Ho (Department of Leisure and Sport, Dong-Eui University)
  • 김도연 (부산대학교 체육교육학과) ;
  • 우진희 (동아대학교 체육학과) ;
  • 신기옥 (동아대학교 체육학과) ;
  • 노희태 (동아대학교 체육학과) ;
  • 이율효 (동아대학교 체육학과) ;
  • 윤병곤 (동의대학교 체육학과) ;
  • 박찬호 (동의대학교 레저스포츠학과)
  • Received : 2020.03.30
  • Accepted : 2020.06.12
  • Published : 2020.06.30
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Abstract

The purpose of this study was to investigate the effects of different types of exercise training on ẞ-Amyloid, Brain-Derived Nerurotrophic Factor(BDNF) and cognitive function in mice with Diabetes Mellitus Group(DM.G). 24 male C57BL/6 mice were randomly assigned to the control (C.G. n = 6) and Diabetes Mellitus Group(DM.G. n = 18) groups. After the Diabetes Mellitus induction period, the DM group was subdivided into DM.G. + sedentary (DM.G., n = 6), DM.G. + endurance exercise (A.G, n = 6), and DM.G. + resistance exercise (R.G., n = 6). The A.G. and R.G performed treadmill and ladder climbing exercises 5 times per week for 8 weeks, respectively. After 8 weeks the results are as follows: ẞ-Amyloid showed higher levels of DM.G. than in A.G., R.G., and C.G., but was not statistically significant(p>.05). BDNF was significantly lower in DM.G. than in C.G., A.G., and R.G.(p <0.05). The Y-maze task performance for cognitive function was significantly lower in DM.G. than in C.G., A.G., and R.G.(p <0.05). These results predict that diabetes can negatively affect ẞ-Amyloid, BDNF and cognitive function. It can also be predicted that low-intensity exercise can positively improve ẞ-Amyloid, BDNF and cognitive function regardless of the type of exercise.

본 연구는 서로 다른 운동유형이 당뇨처치 그룹의 베타 아밀로이드, BDNF 및 인지기능에 미치는 영향을 알아보기 위하여 24마리의 C57BL/6 쥐를 당뇨처치를 하지 않은 그룹 6마리(통제그룹: 6)와 당뇨처치 그룹 18마리로 무작위 할당하여, 이를 다시 당뇨처치 그룹의 경우 통제그룹 6마리, 유산소 운동그룹 6마리, 저항운동 그룹 6마리로 나누었고 운동그룹은 8주간 주 5회 저강도로 각각 트레드밀 운동과 사다리 운동을 실시하였다. 그 결과 베타아밀로이드는 8주 후 당뇨처치 그룹의 통제그룹(DM.G.)이 나머지 세 그룹에 비하여 높은 수준을 나타내었으나 통계적으로 유의한 차이는 나타나지 않았다. BDNF의 경우는 당뇨통제그룹이 나머지 세 그룹에 비하여 낮은 수준을 나타냈으며 통계적으로 유의한 차이를 나타냈다(p<.05). 인지기능을 알아보기 위한 Y-미로 검사에서도 당뇨통제 그룹이 나머지 세그룹에 비하여 낮은 수준을 보였으며 통계적으로도 유의한 차이를 나타냈다(p<.05).

Keywords

References

  1. S. M. Lee, "Effects of the type of exercise pattern on cognitive function, physical functions in elderly patients with dementia." Graduate school of Yongin Univ.(2011).
  2. Mcdowell I., "Alzheimer's disease: Insights from epidemiology", Aging. Vol.13, No.3 pp. 143-162,(2001).
  3. S. H. Lee, "Relationships between Dementia Knowledge, Depression and Dementia Preventive Behaviors among patients with HTN or DM." Graduate school of Chungnam national Univ.,(2015).
  4. Biessels G. J., Deary I. J., Ryan C. M., "Cognightion and diabetes: a lifespan perspective." Lancet Neurol.. Vol.7, pp.184-190,(2008). https://doi.org/10.1016/S1474-4422(08)70021-8
  5. Cheiri C., Staines W., Miki T. Seino, S., Messier, C., "Glucose transporter plasticity during memory processing." Neurosci. Vol.130, pp.591-600,(2005). https://doi.org/10.1016/j.neuroscience.2004.09.011
  6. Gispen W. H., Biessels G. J., "Cognition and synaptic plasticity in diabetes mellitus. Trends." Neurosci. Vol.23, pp.542-549, (2000). https://doi.org/10.1177/1073858416679936
  7. Donovan M. H., Yamaguchi M., Eisch A. J., "Dynamic expression of TrkB receptor protein on proliferation and maturing cells in the adult mouse dentate gyrus." Hippocampus, Vol.18, pp.435-439,(2008). https://doi.org/10.1002/hipo.20410
  8. Komulainen P., Pederson M., Hanninen T., Bruunsgaard H., Lakka T. A., Kivipelto M., "BDNF is a novel marker of cognitive function is aging women: the DR's EXTRA study." Neurobiology of Learning and Memory. Vol.90, No.4, pp.596-603, (2008). https://doi.org/10.1016/j.nlm.2008.07.014
  9. Pietrzit C., Behl C., "Concepts for the treatment of Alzheimer's disease molecular mechanisms and clinical application." Int. J. Exp. Pathol. Vol.86, No.3, pp.173-185, (2005). https://doi.org/10.1111/j.0959-9673.2005.00435.x
  10. Loimaala A., Huikuri H. V., Koobi T., Rinne, M., Nenonen, A., Vuori, I., "Exercise training improves baroreflex sensitivity in type 2 diabetes." Diabetes Vol.52, No.7, pp.1837-1842,(2003). https://doi.org/10.2337/diabetes.52.7.1837
  11. Fontan-Lozano A. Saez-Cassanelli J. L Inda M. C., de los Santos-Arteaga, M., Sierra-Dominguez, S. A., Lopez-Liuch, G., Delgado-Gracia, J.M., Carrion, A.M.. "Caloric restriction increases learning consolidation and facilitates synaptic plasticity through mechanisms dependent on NR2B subunits of NMDA receptor." J. Neurosci. Vol.27, No.38, pp.10185-10210, (2007). https://doi.org/10.1523/JNEUROSCI.2757-07.2007
  12. Chengxin Sun, Xinzhi Li, Lu Liu, Mark J Canet, Yuan Guan, Yuying Fan, Yifa Zhou, Effect of fasting time on measuring mouse blood glucose level. Int J Clin Exp Med. pp.4186-4189.(2016).
  13. Clee S. M, Attie A. D., The genetic landscape of type 2 diabetes in mice. Endocr Rev. pp.48-83.(2007)
  14. Fajardo R. J., Karim L., Calley V.I., Bouxsein M. L., A review of rodent models of type 2 diabetic skeletal fragility. J Bone Miner Res. pp.1025-40.(2014).
  15. I. C. Sanches, F. F. Conti, M. Sartori, M. C. Irigoyen, K. De Angelis. "Standardization of resistance exercise training: effects in diabetic ovariectomized rats" Int J Sports Med, Vol.35, No.4, pp.323-329,(2014). https://doi.org/10.1055/s-0033-1351254
  16. Selkoe, D. J., "Alzheimer's disease: genes, proteins, and therapy." Physiological reviews, Vol. 81, No.2, pp.741-766,(2001). https://doi.org/10.1152/physrev.2001.81.2.741
  17. M. Shinohara, N. Sato, "Bidirectional interactions between diabetes and Alzheimer's disease" Neurochem. Int, Vol.108, pp.296-302,(2017). https://doi.org/10.1016/j.neuint.2017.04.020
  18. P. Bharadwaj, N. Wijesekara, M. Liyanapathirana, P. Newsholme, L. Ittner, P. Fraser, G. Verdile, "The Link between Type 2 Diabetes and Neurodegeneration: Roles for Amyloid-${\beta}$, Amylin, and Tau Proteins." J. Alzheimers Dis., Vol.59, No.2, pp. 421-432,(2017). https://doi.org/10.3233/JAD-161192
  19. S. M. de la Monte, "Type 3 diabetes is sporadic Alzheimer's disease: mini-review" Eur Neuropsychopharmacol, Vol.24, No.12, pp.1954-1960,(2014). https://doi.org/10.1016/j.euroneuro.2014.06.008
  20. Yokoyama, H., Okazaki, K., Imai, D., Yamashina, Y., Takeda, R., Naghavi, N., Ota, A., Hirasawa, Y., & Miyagawa, T., "The effect of cognitive-motor dual-task training on cognitive function and plasma amyloid ${\beta}$ peptide 42/40 ratio in healthy elderly persons: a randomized controlled trial." BMC geriatrics, Vol.15, No.1, p.60,(2015). https://doi.org/10.1186/s12877-015-0058-4
  21. S. K. Kim, "The effect of combined exercise on cognitive function.functional fitness.blood lipids and risk factors of dementia in elderly women." Graduate school of Pusan National Univ.(2011).
  22. J. W. Lee, C. H. Kim, S. I. Han, P. W. Lee, "The effect of Aquatic exercise program in elderly women on the cognitive function and Alzheimer's disease dementia factor." Korean Journal of Physical Education, Vol.51, No.5, pp.627-637,(2012).
  23. C. H. Park, J. U. Cheon, " Effects od 16-week combined exercise on dementia, depression, and cognitive function in elderly women." Journal of Korean applied science and technology, Vol.36, No.2, pp.456-467,(2019). https://doi.org/10.12925/JKOCS.2019.36.2.456
  24. P. A. Adlard, V. M. Perreau, V. Pop, C. W. Cotman, "Voluntary exercise decreases amyloid load in a transgenic model of Alzheimer's disease.", The Journal of Neurosci. Vol.25, No.17, pp.4217-4221, (2005). https://doi.org/10.1523/JNEUROSCI.0496-05.2005
  25. Rasmussen, P., Brassard, P., Adser, H., Pedersen, M. V., Leick, L., Hart, E., Secher, N. H., Perdersen, B. K., Pilegaard, H., "Evidence for a release of brain-derived neurotrophic factor from the brain during exercise." Experimental physiology, Vol.94, No.10, pp.1062-1069,(2009). https://doi.org/10.1113/expphysiol.2009.048512
  26. Pan, W., Banks, W. A., Fasold, M. B., Bluth, J., & Kastin, A. J., "Transport of brain-derived neurotrophic factor across the blood-brain barrier." Neuropharmacology, Vol.37, No.12, pp.1553-1561, (1998). https://doi.org/10.1016/S0028-3908(98)00141-5
  27. Piepmeier, A. T., & Etnier, J. L., "Brain-derived neurotrophic factor(BDNF) as a potential mechanism of the effects of acute exercise on cognitive performance." Journal of Sport and Health Science, Vol.4, No.1, pp.14-23.(2015). https://doi.org/10.1016/j.jshs.2014.11.001
  28. Bekinschtein, P., Cammarota, M., Katche, C., Slipczuk, L., Rossato, J. I., Goldin, A., Izquicrdo, I., Medina, J. H., "BDNF is essential to promote persistence of long-term memory strage." Proceedings of the national Academy of Sciences of the U.S.A. Vol.105, pp.2711-2716,(2008). https://doi.org/10.1073/pnas.0711863105
  29. Hutchinson, K. J., Gomez-Pinilla, F., Crowe, M. J., Ying, Z., Basso, D. M., "Three exercise paradigms differentially improve sensory recovery after spinal cord contusion in rats." Brain, Vol.127, No.6, pp.1403-1414.(2004). https://doi.org/10.1093/brain/awh160
  30. Y. K. Jeon, J. H. Kim, J. I. Chol, "The Change of Brain-Derived Neurotrophic Factor(BDNF), Insulin-Like Growth Factor-1(IGF-1) and Working-Memory in Adolescents by Aerobic Exercise Intensity for 12 weeks." Korean Journal of Physical Education, Vol.52, No.5, pp.753-763, (2013).
  31. B. G. Chol, H. K. Yoon, "The Effects of Aerobic and Thera-Band Combined Exercise on Cerebral Blood Flow, BDNF, and Aging-Related Hormone in Elderly." The Korean Journal of growth and development, Vol.21, No.4, pp.341-346, (2013).
  32. Levinger, I., Goodiman, C., Matthews, V., Hare, D. L., Jerums, G., Gamham, A., Selig, S., "BDNF, Metabolic risk factors and resistance training in middle-aged individuals." Journal of Medicine and Sciences in sports Exercise. Vol.40, No.3, pp.533-541.(2008)
  33. Rojas Vega, S., Knicker, A., Hollmann, W., Bloch, W., Struder, H. K., "Effect of resistance exercise on serum levels of growth factors in humans." Hormone and Metabolic Research. Vol.42, No.13, pp.982-986,(2010). https://doi.org/10.1055/s-0030-1267950
  34. Goekint, M., De Pauw, K., Roelands, B., Njemini, R., Bautmans, I., Met, T., Meeusen, R., "Strength training does not influence serum brain-derived neurotrophic factor." European Journal of Applied Physiology, Vol.110, No.2, pp.285-293, (2010). https://doi.org/10.1007/s00421-010-1461-3
  35. Knaepen, K., Goekint, M., Heyman, E. M., & Meeusen, R., "Neuroplasticity-Exercise-Induced Response of Peripheral Brain-Derived Neurotrophic Factor." Sports Medicine, Vol.40, No.9, pp.765-801, (2010). https://doi.org/10.2165/11534530-000000000-00000
  36. Naissner, U., Cognitive psychology. New York: Appleton-Century-Crofts.(1967).
  37. McMorris, T., Exercise-Cognition Interaction: Neuroscience Perspectives. Elsevier Science.(2015).
  38. Da-Young Gong, Yun-Sik Choi, "Development of New Analytical Method Evaluating Working Memory on Y Maze." J. Life Science. Vol.26, No.2, pp.234-240, (2016). https://doi.org/10.5352/JLS.2016.26.2.234
  39. H. S. Cho, "Effect of aging and treadmill exercise on mitochondrial function and neuronal plasticity in the rat hippocampus." Graduate school of Kyung Hee Univ.(2016).
  40. Angevaren, M., Aufdemkampe, G., Verhaar, H., Aleman, A., & Vanhees, L., "Physical activity and enhanced fitness to improve cognitive function in older people without known cognitive impairment." Cochrane Database Syst Rev, Vol.3, No.3, p.101.(2008).
  41. Smith, P. J., Blumenthal, J. A., Hoffman, B. M., Cooper, H., Strauman, T. A., Welsh-Bohmer, K., Browndyke, J. N., Sherwood, A., "Aerobic exercise and neurocognitive performance: a metaanalytic review of randomized controlled trials." Psychosomatic medicine, Vol.72, No.3, p. 239,.(2010). https://doi.org/10.1097/psy.0b013e3181d14633
  42. Hamer, M., & Chida, Y., "Physical activity and risk of neurodegenerative disease: a systematic review of prospective evidence." Psychological medicine, Vol.39, No.01, pp. 3-11.(2009) https://doi.org/10.1017/s0033291708003681
  43. Sofi, F., Valecchi, D., Bacci, D., Abbate, R., Gensini, G., Casini, A., & Macchi, C., "Physical activity and risk of cognitive decline: a meta-analysis of prospective studies." Journal of internal medicine, Vol.269, No.1, pp.107-117,(2011). https://doi.org/10.1111/j.1365-2796.2010.02281.x