The Plant Pathology Journal

한국식물병리학회 (The Korean Society of Plant Pathology)
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Menadione Sodium Bisulfite-Protected Tomato Leaves against Grey Mould via Antifungal Activity and Enhanced Plant Immunity

  • Jo, Youn Sook (Department of Horticultural Science, Gyeongnam National University of Science and Technology (GNTech)) ;
  • Park, Hye Bin (Department of Horticultural Science, Gyeongnam National University of Science and Technology (GNTech)) ;
  • Kim, Ji Yun (Department of Horticultural Science, Gyeongnam National University of Science and Technology (GNTech)) ;
  • Choi, Seong Min (Department of Horticultural Science, Gyeongnam National University of Science and Technology (GNTech)) ;
  • Lee, Da Sol (Department of Horticultural Science, Gyeongnam National University of Science and Technology (GNTech)) ;
  • Kim, Do Hoon (Department of Horticultural Science, Gyeongnam National University of Science and Technology (GNTech)) ;
  • Lee, Young Hee (Department of Horticultural Science, Gyeongnam National University of Science and Technology (GNTech)) ;
  • Park, Chang-Jin (Department of Bioresources Engineering, Sejong University) ;
  • Jeun, Yong-Chull (College of Applied Life Science, Faculty of Bioscience and Industry, The Research Institute for Subtropical Agriculture and Biotechnology, Jeju National University) ;
  • Hong, Jeum Kyu (Department of Horticultural Science, Gyeongnam National University of Science and Technology (GNTech))
  • 투고 : 2020.06.29
  • 심사 : 2020.07.16
  • 발행 : 2020.08.01
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초록

Tomato grey mould has been one of the destructive fungal diseases during tomato production. Ten mM of menadione sodium bisulfite (MSB) was applied to tomato plants for eco-friendly control of the grey mould. MSB-reduced tomato grey mould in the 3rd true leaves was prolonged at least 7 days prior to the fungal inoculation of two inoculum densities (2 × 104 and 2 × 105 conidia/ml) of Botrytis cinerea. Protection efficacy was significantly higher in the leaves inoculated with the lower disease pressure of conidial suspension compared to the higher one. MSB-pretreatment was not effective to arrest oxalic acid-triggered necrosis on tomato leaves. Plant cell death and hydrogen peroxide accumulation were restricted in necrotic lesions of the B. cinereainoculated leaves by the MSB-pretreatment. Decreased conidia number and germ-tube elongation of B. cinerea were found at 10 h, and mycelial growth was also impeded at 24 h on the MSB-pretreated leaves. MSB-mediated disease suppressions were found in cotyledons and different positions (1st to 5th) of true leaves inoculated with the lower conidial suspension, but only 1st to 3rd true leaves showed decreases in lesion sizes by the higher inoculum density. Increasing MSB-pretreatment times more efficiently decreased the lesion size by the higher disease pressure. MSB led to inducible expressions of defence-related genes SlPR1a, SlPR1b, SlPIN2, SlACO1, SlChi3, and SlChi9 in tomato leaves prior to B. cinerea infection. These results suggest that MSB pretreatment can be a promising alternative to chemical fungicides for environment-friendly management of tomato grey mould.

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참고문헌

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