Restorative Dentistry and Endodontics

The Korean Academy of Conservative Dentistry (대한치과보존학회)
권호 표지
DOI QR코드

DOI QR Code

Bioactivity of endodontic biomaterials on dental pulp stem cells through dentin

  • Javid, Bahar (Department of Restorative Dentistry, School of Dentistry, Shahid Beheshti University of Medical Sciences) ;
  • Panahandeh, Narges (Department of Restorative Dentistry, School of Dentistry, Shahid Beheshti University of Medical Sciences) ;
  • Torabzadeh, Hassan (Dental Research Center, Research Institute of Dental Sciences, Shahid Beheshti University of Medical Sciences) ;
  • Nazarian, Hamid (Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences) ;
  • Parhizkar, Ardavan (Iranian Center for Endodontic Research, Research Institute of Dental Sciences, Shahid Beheshti University of Medical Sciences) ;
  • Asgary, Saeed (Iranian Center for Endodontic Research, Research Institute of Dental Sciences, Shahid Beheshti University of Medical Sciences)
  • Received : 2019.06.17
  • Accepted : 2019.09.14
  • Published : 2020.02.29
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives: This study investigated the indirect effect of calcium-enriched mixture (CEM) cement and mineral trioxide aggregate (MTA), as 2 calcium silicate-based hydraulic cements, on human dental pulp stem cells (hDPSCs) through different dentin thicknesses. Materials and Methods: Two-chamber setups were designed to simulate indirect pulp capping (IPC). Human molars were sectioned to obtain 0.1-, 0.3-, and 0.5-mm-thick dentin discs, which were placed between the 2 chambers to simulate an IPC procedure. Then, MTA and CEM were applied on one side of the discs, while hDPSCs were cultured on the other side. After 2 weeks of incubation, the cells were removed, and cell proliferation, morphology, and attachment to the discs were evaluated under scanning electron microscopy (SEM). Energy-dispersive X-ray (EDXA) spectroscopy was performed for elemental analysis. Alkaline phosphatase (ALP) activity was assessed quantitatively. The data were analyzed using the Kruskal-Wallis and Mann-Whitney tests. Results: SEM micrographs revealed elongated cells, collagen fibers, and calcified nucleations in all samples. EDXA verified that the calcified nucleations consisted of calcium phosphate. The largest calcifications were seen in the 0.1-mm-thick dentin subgroups. There was no significant difference in ALP activity across the CEM subgroups; however, ALP activity was significantly lower in the 0.1-mm-thick dentin subgroup than in the other MTA subgroups (p < 0.05). Conclusions: The employed capping biomaterials exerted biological activity on hDPSCs, as shown by cell proliferation, morphology, and attachment and calcific precipitations, through 0.1- to 0.5-mm-thick layers of dentin. In IPC, the bioactivity of these endodontic biomaterials is probably beneficial.

Keywords

References

  1. Thompson V, Craig RG, Curro FA, Green WS, Ship JA. Treatment of deep carious lesions by complete excavation or partial removal: a critical review. J Am Dent Assoc 2008;139:705-712. https://doi.org/10.14219/jada.archive.2008.0252
  2. Schwendicke F, Frencken JE, Bjorndal L, Maltz M, Manton DJ, Ricketts D, Van Landuyt K, Banerjee A, Campus G, Domejean S, Fontana M, Leal S, Lo E, Machiulskiene V, Schulte A, Splieth C, Zandona AF, Innes NP. Managing carious lesions: consensus recommendations on carious tissue removal. Adv Dent Res 2016;28:58-67. https://doi.org/10.1177/0022034516639271
  3. Ricketts D. Management of the deep carious lesion and the vital pulp dentine complex. Br Dent J 2001;191:606-610. https://doi.org/10.1038/sj.bdj.4801246
  4. Chogle SM, Goodis HE, Kinaia BM. Pulpal and periradicular response to caries: current management and regenerative options. Dent Clin North Am 2012;56:521-536. https://doi.org/10.1016/j.cden.2012.05.003
  5. Gruythuysen RJ, van Strijp AJ, Wu MK. Long-term survival of indirect pulp treatment performed in primary and permanent teeth with clinically diagnosed deep carious lesions. J Endod 2010;36:1490-1493. https://doi.org/10.1016/j.joen.2010.06.006
  6. Asgary S, Hassanizadeh R, Torabzadeh H, Eghbal MJ. Treatment outcomes of 4 vital pulp therapies in mature molars. J Endod 2018;44:529-535. https://doi.org/10.1016/j.joen.2017.12.010
  7. Bergenholtz G. Factors in pulpal repair after oral exposure. Adv Dent Res 2001;15:84. https://doi.org/10.1177/08959374010150012201
  8. Asgary S, Eghbal MJ, Bagheban AA. Long-term outcomes of pulpotomy in permanent teeth with irreversible pulpitis: a multi-center randomized controlled trial. Am J Dent 2017;30:151-155.
  9. Fallahinejad Ghajari M, Asgharian Jeddi T, Iri S, Asgary S. Treatment outcomes of primary molars direct pulp capping after 20 months: a randomized controlled trial. Iran Endod J 2013;8:149-152.
  10. Araujo LB, Cosme-Silva L, Fernandes AP, Oliveira TM, Cavalcanti BD, Gomes Filho JE, Sakai VT. Effects of mineral trioxide aggregate, $Biodentine^{TM}$ and calcium hydroxide on viability, proliferation, migration and differentiation of stem cells from human exfoliated deciduous teeth. J Appl Oral Sci 2018;26:e20160629. https://doi.org/10.1590/1678-7757-2016-0629
  11. Agrafioti A, Taraslia V, Chrepa V, Lymperi S, Panopoulos P, Anastasiadou E, Kontakiotis EG. Interaction of dental pulp stem cells with Biodentine and MTA after exposure to different environments. J Appl Oral Sci 2016;24:481-486. https://doi.org/10.1590/1678-775720160099
  12. Kamali A, Javadpour S, Javid B, Kianvash Rad N, Naddaf Dezfuli S. Effects of chitosan and zirconia on setting time, mechanical strength, and bioactivity of calcium silicate-based cement. Int J Appl Ceram Technol 2017;14:135-144. https://doi.org/10.1111/ijac.12636
  13. Kim M, Kim S, Ko H, Song M. Effect of ProRoot MTA(R) and Biodentine(R) on osteoclastic differentiation and activity of mouse bone marrow macrophages. J Appl Oral Sci 2019;27:e20180150. https://doi.org/10.1590/1678-7757-2018-0150
  14. Moazzami F, Ghahramani Y, Tamaddon AM, Dehghani Nazhavani A, Adl A. A histological comparison of a new pulp capping material and mineral trioxide aggregate in rat molars. Iran Endod J 2014;9:50-55.
  15. Parirokh M, Torabinejad M. Mineral trioxide aggregate: a comprehensive literature review--part III: clinical applications, drawbacks, and mechanism of action. J Endod 2010;36:400-413. https://doi.org/10.1016/j.joen.2009.09.009
  16. Tabarsi B, Parirokh M, Eghbal MJ, Haghdoost AA, Torabzadeh H, Asgary S. A comparative study of dental pulp response to several pulpotomy agents. Int Endod J 2010;43:565-571. https://doi.org/10.1111/j.1365-2591.2010.01711.x
  17. Tak O, Usumez A. Diffusion of HEMA from resin cements through different dentin thicknesses in vitro. Am J Dent 2015;28:285-291.
  18. Murray PE, About I, Lumley PJ, Franquin JC, Remusat M, Smith AJ. Cavity remaining dentin thickness and pulpal activity. Am J Dent 2002;15:41-46.
  19. Asgary S, Nazarian H, Khojasteh A, Shokouhinejad N. Gene expression and cytokine release during odontogenic differentiation of human dental pulp stem cells induced by 2 endodontic biomaterials. J Endod 2014;40:387-392. https://doi.org/10.1016/j.joen.2013.09.017
  20. Rahimi S, Mokhtari H, Shahi S, Kazemi A, Asgary S, Eghbal MJ, Mesgariabbasi M, Mohajeri D. Osseous reaction to implantation of two endodontic cements: mineral trioxide aggregate (MTA) and calcium enriched mixture (CEM). Med Oral Patol Oral Cir Bucal 2012;17:e907-e911.
  21. Asgary S, Eghbal MJ, Ehsani S. Periradicular regeneration after endodontic surgery with calcium-enriched mixture cement in dogs. J Endod 2010;36:837-841. https://doi.org/10.1016/j.joen.2010.03.005
  22. Zarrabi MH, Javidi M, Jafarian AH, Joushan B. Histologic assessment of human pulp response to capping with mineral trioxide aggregate and a novel endodontic cement. J Endod 2010;36:1778-1781. https://doi.org/10.1016/j.joen.2010.08.024
  23. Asgary S, Parirokh M, Eghbal MJ, Ghoddusi J. SEM evaluation of pulp reaction to different pulp capping materials in dog's teeth. Iran Endod J 2006;1:117-123.
  24. Sarkar NK, Caicedo R, Ritwik P, Moiseyeva R, Kawashima I. Physicochemical basis of the biologic properties of mineral trioxide aggregate. J Endod 2005;31:97-100. https://doi.org/10.1097/01.DON.0000133155.04468.41
  25. Asgary S, Eghbal MJ, Parirokh M, Ghoddusi J. Effect of two storage solutions on surface topography of two root-end fillings. Aust Endod J 2009;35:147-152. https://doi.org/10.1111/j.1747-4477.2008.00137.x
  26. Tomson PL, Grover LM, Lumley PJ, Sloan AJ, Smith AJ, Cooper PR. Dissolution of bio-active dentine matrix components by mineral trioxide aggregate. J Dent 2007;35:636-642. https://doi.org/10.1016/j.jdent.2007.04.008
  27. Aubin JE, Triffitt JT. Chapter 4 - mesenchymal stem cells and osteoblast differentiation. In: Bilezikian JP, Raisz LG, Rodan GA, editors. Principles of bone biology. 2nd ed. San Diego, CA: Academic Press; 2002. p59-81.
  28. Kim MJ, Kim KN, Lee YK, Kim KM. Cytotoxicity test of dentin bonding agents using millipore filters as dentin substitutes in a dentin barrier test. Clin Oral Investig 2013;17:1489-1496. https://doi.org/10.1007/s00784-012-0840-z
  29. Sengun A, Yalcin M, Ulker HE, Ozturk B, Hakki SS. Cytotoxicity evaluation of dentin bonding agents by dentin barrier test on 3-dimensional pulp cells. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2011;112:e83-e88.
  30. Dunne SM. The limitation of visual perception in restorative dentistry. Dent Update 1993;20:198-201.
  31. Camps J, Dejou J, Remusat M, About I. Factors influencing pulpal response to cavity restorations. Dent Mater 2000;16:432-440. https://doi.org/10.1016/S0109-5641(00)00041-5