The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
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The Effect of Galkunhwanglyeonhwanggum-tang (GGT) and Sopunghwalhyeol-tang (SPT) on Gene Expression Levels of MCP-1, ICAM-1, VCAM-1, and eNOS in HUVECs

갈근황연황금탕(葛根黃蓮黃芩湯)과 소풍활혈탕(疎風活血湯)이 HUVEC 내에 MCP-1, ICAM-1, VCAM-1 and eNOS의 유전자 발현량에 대해 미치는 영향

  • Jeong, Hyun-jin (Dept. of Internal Korean Medicine, Gwangju Dong-Shin University Oriental Hospital) ;
  • Jeon, Sang-yoon (Dept. of Internal Korean Medicine, Mokpo Dong-Shin University Oriental Hospital) ;
  • Jang, Hye-yeon (Dept. of Internal Korean Medicine, Gwangju Dong-Shin University Oriental Hospital) ;
  • Kim, Min-wook (Dept. of Acupuncture & Moxibustion Medicine, Gwangju Dong-Shin University Oriental Hospital)
  • 정현진 (동신대학교 광주한방병원 한방내과) ;
  • 전상윤 (동신대학교 목포한방병원 한방내과) ;
  • 장혜연 (동신대학교 광주한방병원 한방내과) ;
  • 김민욱 (동신대학교 광주한방병원 한방침구의학과)
  • Received : 2020.09.01
  • Accepted : 2020.09.29
  • Published : 2020.09.30
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Abstract

Objectives: The aim of this study was to compare the effects of Galkunhwanglyeonhwanggum-tang (GGT), and Sopunghwalhyeol-tang (SPT) on gene expression of MCP-1, ICAM-1, VCAM-1, and eNOS in human umbilical vein endothelial cells (HUVECs). Methods: HUVECs were treated with GGT and SPT at concentrations of 50, 100, and 200 ㎍/mL. Gene expression of MCP-1, ICAM-1, VCAM-1, and eNOS in HUVECs was analyzed by the polymerase chain reaction (PCR), and electrophoresis was performed to verify the gene expression level. Results: 1. MCP-1 gene expression was more strongly decreased by SPT than by GGT. 2. ICAM-1 and VCAM-1 gene expressions were more strongly decreased by SPT than by GGT 3. GGT significantly increased eNOS gene expression, but SPT did not. Conclusions: These findings suggest that GGT and SPT regulate gene expression related to anti-inflammatory effects in HUVECs. Clinical application of these Korean medicines to diseases related to dyslipidemia, such as cardiovascular disease, will require additional in vivo experiments to verify the anti-inflammatory effects of GGT and SPT.

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