Journal of Convergence for Information Technology (융합정보논문지)

Convergence Society for SMB (중소기업융합학회)
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Antioxidant Activity and DNA Protective Effect against Oxidative Stress of Pinus rigida × taeda Cone

리기테다 소나무 솔방울의 항산화 활성 및 산화적 DNA 손상에 대한 억제 효과

  • Choi, Jisoo (Dept. of Pharmaceutical Science, Jungwon University) ;
  • Jang, Taewon (Dept. of Medicinal Plant Resources, Andong National University) ;
  • Min, Youngsil (Dept. of Pharmaceutical Science, JungWon University) ;
  • Lee, Manhyo (Hemp Promotion Project Team, Gyeongbuk Institute for Bio Industry) ;
  • Park, Jaeho (Dept. of Pharmaceutical Science & Institute of International Agricultural Research, JungWon Univ.)
  • 최지수 (중원대학교 제약공학과) ;
  • 장태원 (안동대학교 생약자원학과) ;
  • 민영실 (중원대학교 제약공학과) ;
  • 이만효 (경북바이오산업연구원) ;
  • 박재호 (중원대학교 제약공학과 및 중원대학교 국제유기농산업연구소)
  • Received : 2020.09.09
  • Accepted : 2020.11.20
  • Published : 2020.11.28
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Abstract

Reactive oxygen species (ROS) damage DNA and cause cancer. Therefore, the research is being conducted on the development of antioxidants for the removal of ROS. This study was performed to investigate antioxidant activity and protective effect against oxidative DNA damage using ethyl acetate fractions from the cone of Pinus rigida × taeda (ERT). The antioxidant activity was evaluated using the DPPH, ABTS radical scavenging assay, reducing power assay, and Fe2+ chelating assay. Also, the contents of phenolic compounds and vitamin C related to antioxidant activity were analyzed to confirm phytochemicals. The DNA protective effect against oxidative stress was confirmed by the φX-174 RF I plasmid DNA cleavage assay. As a result, ERT showed DPPH and ABTS radical scavenging activities in a concentration-dependent manner. The results of reducing power and Fe2+ chelating activities were 77.32 ± 2.28% and 64.09 ± 1.01% at 200 ㎍/㎖. Also, ERT showed a DNA protective effect against oxidative stress.

활성산소종이 DNA를 손상하고 암을 유발하는데 기인하는 것으로 밝혀지면서 활성산소를 제거하기 위한 항산화 물질 개발 연구가 진행되고 있다. 본 연구에서는 리기테다 소나무 솔방울 에틸아세테이트 분획물의 항산화 효과 및 산화적 스트레스에 의해 야기된 DNA 손상 보호 효과를 조사하기 위해 수행되었다. 항산화 활성을 확인하기 위해 DPPH 및 ABTS 라디칼 소거 활성, 환원력, Fe2+ 킬레이팅 활성을 평가하였으며, 항산화 활성과 연관된 총 페놀 및 비타민 C의 함량도 분석하여 식물 화학물질을 확인하였다. 산화적 DNA 손상 억제 효과는 φX-174 RF I plasmid DNA 절단 분석법을 이용하여 측정하였다. DPPH 및 ABTS 라디칼 소거 활성은 농도 의존적으로 나타났다. 환원력과 Fe2+ 킬레이팅 활성은 200 ㎍/㎖에서 각각 77.32 ± 2.28%, 64.09 ± 1.01%의 활성을 나타냈다. 또한, 리기테다 소나무 솔방울은 산화적 스트레스에 대한 plasmid DNA 보호 효과를 보였다.

Keywords

References

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