KOREAN JOURNAL OF CROP SCIENCE (한국작물학회지)

The Korean Society of Crop Science (한국작물학회)
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Evaluation of Morphological Traits and Genetic Composition in Melon Germplasm

멜론 유전자원의 형태적 특성 및 유전적 구성 평가

  • Lee, Seungbum (National Agrobiodiversity Center, National Institute of Agricultural Sciences (NAS), RDA) ;
  • Jang, Ik (National Agrobiodiversity Center, National Institute of Agricultural Sciences (NAS), RDA) ;
  • Hyun, Do Yoon (National Agrobiodiversity Center, National Institute of Agricultural Sciences (NAS), RDA) ;
  • Lee, Jung-Ro (National Agrobiodiversity Center, National Institute of Agricultural Sciences (NAS), RDA) ;
  • Kim, Seong-Hoon (National Agrobiodiversity Center, National Institute of Agricultural Sciences (NAS), RDA) ;
  • Yoo, Eunae (National Agrobiodiversity Center, National Institute of Agricultural Sciences (NAS), RDA) ;
  • Lee, Sookyeong (National Agrobiodiversity Center, National Institute of Agricultural Sciences (NAS), RDA) ;
  • Cho, Gyu-Taek (National Agrobiodiversity Center, National Institute of Agricultural Sciences (NAS), RDA) ;
  • Lee, Kyung Jun (National Agrobiodiversity Center, National Institute of Agricultural Sciences (NAS), RDA)
  • 이승범 (농촌진흥청 국립농업과학원 농업유전자원센터) ;
  • 장익 (농촌진흥청 국립농업과학원 농업유전자원센터) ;
  • 현도윤 (농촌진흥청 국립농업과학원 농업유전자원센터) ;
  • 이정로 (농촌진흥청 국립농업과학원 농업유전자원센터) ;
  • 김성훈 (농촌진흥청 국립농업과학원 농업유전자원센터) ;
  • 유은애 (농촌진흥청 국립농업과학원 농업유전자원센터) ;
  • 이수경 (농촌진흥청 국립농업과학원 농업유전자원센터) ;
  • 조규택 (농촌진흥청 국립농업과학원 농업유전자원센터) ;
  • 이경준 (농촌진흥청 국립농업과학원 농업유전자원센터)
  • Received : 2020.07.08
  • Accepted : 2020.09.14
  • Published : 2020.12.01
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Abstract

Melon (Cucumis melo L.), one of the most important fruit crop species, is cultivated worldwide. In this study, a total of 206 melon accessions conserved at the National Agrobiodiversity Center (NAC) in RDA were characterized for nine morphological characteristics according to the NAC descriptor list. In addition, to confirm the genetic composition of each melon accession, genetic profiling was performed using 20 SSR markers. Among the 206 melon accessions, 159 (77.2%) were collected from Asia. The color of fruit flesh and skin were mostly 'white' (56.0%) and 'green' (49%), respectively. Days to female flowering (FD) and maturity (MD) of the accessions ranged from 58 to 72 and 17 to 63, respectively. The fruit length and width of the accessions ranged from 6.0 to 29.3 and 3.6 to 17.2 cm, respectively. The sugar content (SU) ranged from 2.5% to 13.2% with an average of 7.0%. In correlation analysis, SU showed positive and negative correlations with MD and FD, respectively. The accessions were classified into four clusters by cluster analysis. From the results of genetic profiling using 20 SSR markers, three accessions (K189118, K100486, and K190292) were expected to be inbred lines among 206 melon accessions. These results could expand the knowledge of the melon germplasm, providing valuable material for the development of new melon varieties to suit consumer tastes.

농업유전자원센터에 보존 중인 멜론(Cucumis melo L.) 206자원에 대한 형태적 특성을 분석하였다. 형태적 특성은 과장, 과폭, 과형지수, 과육두께, 당도, 개화일수, 성숙일수 등 7가지 양적형질과 과피색, 과육색 등 2가지 질적형질을 조사하고 다변량분석을 하였다. 또한 20개 SSR 마커를 이용하여 그들의 유전적 구성을 분석하였다. 1. 멜론 유전자원 중 아시아 국가에서 수집한 자원이 77.2%로 가장 많았으며 국가별로는 우즈베키스탄에서 수집한 자원이 가장 많았다. 2. 7가지 양적형질중에서 개화일수가 자원간 가장 적은 변이(CV=9.2%)를 보였으며 당도(CV=29.1%)가 가장 높은 변이를 보였다. 3. 질적 형질 중 과피색은 녹색(102자원, 49%), 과육색은 흰색(115자원, 56%)이 가장 많았다. 4. 주성분 분석 결과 제1주성분(36.7%), 제2주성분(22.1%), 제3주성분(21.7%)이 전체 변량의 80.5%를 나타내었다. 5. 군집분석 결과 멜론 206자원은 3개의 그룹으로 나뉘었으며 각각 107자원, 43자원, 56자원으로 구성이 되었다. 이 중 그룹 I에 속한 자원들이 다른 그룹의 자원들 보다 과장, 과폭이 길고 당도가 높은 특징을 보였다. 6. SSR 마커를 이용한 유전적 상태 구분 결과 K189118, K100486, K190292은 모두 동형접합형 대립유전자(Homozygote allele)를 보였으며 이를 토대로 근교계(Inbred) 자원으로 예측 할 수 있었다. 본 연구 결과는 멜론 과일 품질 향상을 위한 육종 프로그램에 기초자료로 활용될 수 있을 것이다.

Keywords

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