Journal of Korean Medicine Rehabilitation (한방재활의학과학회지)

The society of Korean Medicine Rehabilitation (한방재활의학과학회)
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Anti-Arthritic Effect of Sogyunghwalhyel-tang-gamibang

소경활혈탕가미방(疎經活血湯加味方)의 관절염에 미치는 효과

  • Jo, Joo-hyun (Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine, Wonkwang University) ;
  • Im, Ji-sung (Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine, Wonkwang University) ;
  • Kim, Jong-gyu (Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine, Wonkwang University) ;
  • Park, Jung-hyun (Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine, Wonkwang University) ;
  • Choi, Hag-soon (Department of Third Medicine of Korean Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Hwang, Geu-won (Department of Third Medicine of Korean Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Song, Yung-sun (Department of Third Medicine of Korean Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University)
  • 조주현 (원광대학교 한의과대학 한방재활의학교실) ;
  • 임지성 (원광대학교 한의과대학 한방재활의학교실) ;
  • 김종규 (원광대학교 한의과대학 한방재활의학교실) ;
  • 박중현 (원광대학교 한의과대학 한방재활의학교실) ;
  • 최학순 (원광대학교 한의학전문대학원 제3의학과) ;
  • 황규원 (원광대학교 한의학전문대학원 제3의학과) ;
  • 송용선 (원광대학교 한의학전문대학원 제3의학과)
  • Received : 2020.12.15
  • Accepted : 2021.01.14
  • Published : 2021.01.31
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Abstract

Objectives The aim of this study is to evaluate anti-inflammatory and anti-arthritic effects of Sogyunghwalhyel-tang-gamibang (SGHHTGB) in cell and animal models and also to suggest one of putative mechanisms underlying its anti-arthritic effects. Methods Enzyme-linked immunosorbent assay was applied to measure the concentrations of tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-6 and prostaglandin E2 (PGE2) in culture medium and blood serum and nitric oxide (NO) was assayed by Griess reagent. The expressions of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) were analyzed by Western blot method. Results In a cell model using RAW264.7 macrophages stimulated with the endotoxin lipopolysaccharide (LPS), the drug, at its non-cytotoxic concentrations, inhibited the production of the pro-inflammatory cytokine TNF-α, IL-1β and IL-6. In addition, it suppressed the expression of the inflammatory enzyme iNOS and COX-2, and reduced the synthesis of the enzyme product NO (as stable nitrite) and PGE2 in activated macrophages. Meanwhile, in an animal model using rheumatic arthritis (RA) mice induced with injection of type II collagen antibody (CAb) and LPS, the drug improved clinical symptom of arthritis and reduced paw thickness and inflammatory cell infiltration. In blood of RA mice, the drug reduced serum levels of TNF-α, IL-1β, IL-6, nitrite, and PGE2, all inflammatory mediators produced by activated macrophages. Conclusions SGHHTGB may ameliorate CAb and LPS-induced RA in mice, presumably by inactivating macrophages that are capable of initiating joint inflammation by producing pro-inflammatory cytokines and expressing inflammatory enzymes.

Keywords

Acknowledgement

본 연구는 2020년도 원광대학교 교내연구비 지원의 결과입니다.

References

  1. Pap T, Korb-Pap A. Cartilage damage in osteoarthritis and rheumatoid arthritis - two unequal siblings. Nat Rev Rheumatol. 2015;11(10):606-15. https://doi.org/10.1038/nrrheum.2015.95
  2. Tadman J, Hill J. The management of osteoarthritis and rheumatoid arthritis. Nurs Times. 2005;101(2):28-9.
  3. Scott DL, Wolfe F, Huizinga TW. Rheumatoid arthritis. Lancet. 2010;376(9746):1094-108. https://doi.org/10.1016/S0140-6736(10)60826-4
  4. McInnes IB, Schett G. The pathogenesis of rheumatoid arthritis. N Engl J Med. 2011;365(23):2205-19. https://doi.org/10.1056/NEJMra1004965
  5. Sharif K, Sharif A, Jumah F, Oskouian R, Tubbs RS. Rheumatoid arthritis in review: Clinical, anatomical, cellular and molecular points of view. Clin Anat. 2018; 31(2):216-23. https://doi.org/10.1002/ca.22980
  6. Noack M, Miossec P. Selected cytokine pathways in rheumatoid arthritis. Semin Immunopathol. 2017;39(4): 365-83. https://doi.org/10.1007/s00281-017-0619-z
  7. Siouti E, Andreakos E. The many facets of macrophages in rheumatoid arthritis. Biochem Pharmacol. 2019;165: 152-69. https://doi.org/10.1016/j.bcp.2019.03.029
  8. Udalova IA, Mantovani A, Feldmann M. Macrophage heterogeneity in the context of rheumatoid arthritis. Nat Rev Rheumatol. 2016;12(8):472-85. https://doi.org/10.1038/nrrheum.2016.91
  9. Tardito S, Martinelli G, Soldano S, Paolino S, Pacini G, Patane M, Alessandri E, Smith V, Cutolo M. Macrophage M1/M2 polarization and rheumatoid arthritis: a systematic review. Autoimmun Rev. 2019;18(11):102397. https://doi.org/10.1016/j.autrev.2019.102397
  10. Szekanecz Z, Koch AE. Macrophages and their products in rheumatoid arthritis. Curr Opin Rheumatol. 2007;19(3):289-95. https://doi.org/10.1097/BOR.0b013e32805e87ae
  11. Schett G, Gravallese E. Bone erosion in rheumatoid arthritis: mechanisms, diagnosis and treatment. Nat Rev Rheumatol. 2012;8(11):656-64. https://doi.org/10.1038/nrrheum.2012.153
  12. Shim JH, Stavre Z, Gravallese EM. Bone loss in rheumatoid arthritis: basic mechanisms and clinical implications. Calcif Tissue Int. 2018;102(5):533-46. https://doi.org/10.1007/s00223-017-0373-1
  13. Vervoordeldonk MJ, Tak PP. Cytokines in rheumatoid arthritis. Curr Rheumatol Rep. 2002;4(3):208-17. https://doi.org/10.1007/s11926-002-0067-0
  14. Del Grossi Moura M, Cruz Lopes L, Silva MT, Barberato-Filho S, Motta RHL, Bergamaschi CC. Use of steroid and nonsteroidal anti-inflammatories in the treatment of rheumatoid arthritis: Systematic review protocol. Medicine (Baltimore). 2018;97(41):e12658. https://doi.org/10.1097/MD.0000000000012658
  15. Abbasi M, Mousavi MJ, Jamalzehi S, Alimohammadi R, Bezvan MH, Mohammadi H, Aslani S. Strategies toward rheumatoid arthritis therapy; the old and the new. J Cell Physiol. 2019;234(7):10018-31. https://doi.org/10.1002/jcp.27860
  16. Ferreira JF, Ahmed Mohamed AA, Emery P. Glucocorticoids and rheumatoid arthritis. Rheum Dis Clin North Am. 2016;42(1):33-vii. https://doi.org/10.1016/j.rdc.2015.08.006
  17. Udalova I, Monaco C, Nanchahal J, Feldmann M. Anti-TNF therapy. Microbiol Spectr. 2016;4(4). doi:10.1128/microbiolspec.MCHD-0022-2015.
  18. Radner H, Aletaha D. Anti-TNF in rheumatoid arthritis: an overview. Wien Med Wochenschr. 2015;165(1-2):3-9. https://doi.org/10.1007/s10354-015-0344-y
  19. Rubbert-Roth A, Atzeni F, Masala IF, Caporali R, Montecucco C, Sarzi-Puttini P. TNF inhibitors in rheumatoid arthritis and spondyloarthritis: Are they the same? Autoimmun Rev. 2018;17(1):24-8. https://doi.org/10.1016/j.autrev.2017.11.005
  20. Wang W, Zhou H, Liu L. Side effects of methotrexate therapy for rheumatoid arthritis: A systematic review. Eur J Med Chem. 2018;158:502-16. https://doi.org/10.1016/j.ejmech.2018.09.027
  21. Burmester GR, Pope JE. Novel treatment strategies in rheumatoid arthritis. Lancet. 2017;389(10086):2338-48. https://doi.org/10.1016/S0140-6736(17)31491-5
  22. Evans PJ, Halliwell B. Side-effects of drugs used in the treatment of rheumatoid arthritis. Biochem Soc Symp. 1995;61:195-207. https://doi.org/10.1042/bss0610195
  23. Dudics S, Langan D, Meka RR, Venkatesha SH, Berman BM, Che CT, Moudgil KD. Natural products for the treatment of autoimmune arthritis: their mechanisms of action, targeted delivery, and interplay with the host microbiome. Int J Mol Sci. 2018;19(9):2508. https://doi.org/10.3390/ijms19092508
  24. The Society of Korean Medicine Rehabilitation. Korean Medicine Rehabilitation. 3rd ed. Soul:Koonja Publisher. 2011:84-6, 98-100.
  25. Zhang ZG. GeumGueyoryakbangron. Taipei: Daelyengookpyung Publisher. 1973:32-3.
  26. Ju JH. Danguesimbub. Soul:Daesung Publisher. 1982:206.
  27. Kwon JS. Paresthesia. Soul:Jeongdam. 1993:207-8, 214-5, 219-22, 270.
  28. Gong JH. Manbyeonghoichun. Soul:Golimssoewon. 1972:5357.
  29. Zhu MS. Euimunbogam. Soul:Golimseowon. 1971:224-7.
  30. Shen JA. Shenshizunshengshu. Taipei:Jayu Publisher. 1980:309-17.
  31. Heo J. Dongyibogam. Soul:Namsandang Publisher. 1989:466-72, 365-78.
  32. Kim JH, Rhee HK. An experimental study on the anti-inflammatory and analgesic effects of Sokyunghwalhyel-Tang. Journal of Korean Medicine. 1985;6(1):133-9.
  33. Kim KY, Jeong HW, Choi CH, Kim HW, Kim GD, Sim KC, Kim GY. Analgesic effects of Sokyungwhalhyul-tang on constriction nerve injury induced neuropathic pain in rats. Journal of Physiology & Pathology in Korean Medicine. 2011;25(2):195-201.
  34. Lee JW, Oh MS. Immunity effect of the Sogyunghwalhyeltanggami in collagen-induced arthritis mice. Journal of Physiology & Pathology in Korean Medicine. 2005;19(5):1323-9.
  35. Kim SJ. Experimental study of Sogyunghwalheyl-tang hap Hwangryunhaedok-tang on the adjuvant arthritis in rats [dissertation]. Daejeon:Daejeon University; 1992.
  36. The College of Korean Medicine Herbal Medicine Common Textbooks Compilation Committee. Herbal medicine. Seoul:Younglim Publisher. 2004:725.
  37. Ricard-Blum S. The collagen family. Cold Spring Harb Perspect Biol. 2011;3(1):a004978. https://doi.org/10.1101/cshperspect.a004978
  38. Shoulders MD, Raines RT. Collagen structure and stability. Annu Rev Biochem. 2009;78:929-58. https://doi.org/10.1146/annurev.biochem.77.032207.120833
  39. Gencoglu H, Orhan C, Sahin E, Sahin K. Undenatured type II collagen (UC-II) in joint health and disease: a review on the current knowledge of companion animals. Animals (Basel). 2020;10(4):697. https://doi.org/10.3390/ani10040697
  40. Kim WU, Cho ML, Jung YO, Min SY, Park SW, Min DJ, Yoon JH, Kim HY. Type II collagen autoimmunity in rheumatoid arthritis. Am J Med Sci. 2004;327(4): 202-11. https://doi.org/10.1097/00000441-200404000-00006
  41. Park KS, Park MJ, Cho ML, Kwok SK, Ju JH, Ko HJ, Park SH, Kim HY. Type II collagen oral tolerance; mechanism and role in collagen-induced arthritis and rheumatoid arthritis. Mod Rheumatol. 2009;19(6): 581-9. https://doi.org/10.3109/s10165-009-0210-0
  42. Bessis N, Decker P, Assier E, Semerano L, Boissier MC. Arthritis models: usefulness and interpretation. Semin Immunopathol. 2017;39(4):469-86. https://doi.org/10.1007/s00281-017-0622-4
  43. Caplazi P, Baca M, Barck K, Carano RAD, DeVoss J, Lee WP, Bolon B, Diehl L. Mouse models of rheumatoid arthritis. Vet Pathol. 2015;52(5):819-26. https://doi.org/10.1177/0300985815588612
  44. Cho YG, Cho ML, Min SY, Kim HY. Type II collagen autoimmunity in a mouse model of human rheumatoid arthritis. Autoimmun Rev. 2007;7(1):65-70. https://doi.org/10.1016/j.autrev.2007.08.001
  45. Dionne RA, Khan AA, Gordon SM. Analgesia and COX-2 inhibition. Clin Exp Rheumatol. 2001;19(6 Suppl 25):S63-S70.
  46. Sener AG, Afsar I. Infection and autoimmune disease. Rheumatol Int. 2012;32(11):3331-8. https://doi.org/10.1007/s00296-012-2451-z