Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Functional screening of Asparagus officinalis L. stem and root extracts

아스파라거스 줄기 및 뿌리 추출물의 기능성 검증

  • Han, Joon-Hee (Department of Research and Development, Chuncheon Bioindustry Foundation) ;
  • Hong, Min (Department of Research and Development, Chuncheon Bioindustry Foundation) ;
  • Lee, Jaehak (Department of Applied Animal Science, College of Animal Life Sciences, Kangwon National University) ;
  • Choi, Da-Hye (Department of Research and Development, Chuncheon Bioindustry Foundation) ;
  • Lee, Sun-Yeop (Department of Research and Development, Chuncheon Bioindustry Foundation) ;
  • Kwon, Tae-Hyung (Department of Research and Development, Chuncheon Bioindustry Foundation) ;
  • Lee, Jae-Hee (Agro-food Research Institute, Gangwondo Agricultural Research and Extension Services (ARES)) ;
  • Lee, Yong-Jin (Department of Research and Development, Chuncheon Bioindustry Foundation) ;
  • Yu, Keun-Hyung (Department of Research and Development, Chuncheon Bioindustry Foundation)
  • 한준희 ((재)춘천바이오산업진흥원 기술개발실) ;
  • 홍민 ((재)춘천바이오산업진흥원 기술개발실) ;
  • 이재학 (강원대학교 동물생명과학대학 동물식품응용학과) ;
  • 최다혜 ((재)춘천바이오산업진흥원 기술개발실) ;
  • 이선엽 ((재)춘천바이오산업진흥원 기술개발실) ;
  • 권태형 ((재)춘천바이오산업진흥원 기술개발실) ;
  • 이재희 (강원도농업기술원 농식품연구소) ;
  • 이용진 ((재)춘천바이오산업진흥원 기술개발실) ;
  • 유근형 ((재)춘천바이오산업진흥원 기술개발실)
  • Received : 2020.12.03
  • Accepted : 2020.12.16
  • Published : 2021.02.28
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Abstract

The biological activities of non-edible extracts of asparagus stems and roots were investigated using hot water and ethanol. The highest contents of rutin and total polyphenol were 31.74 mg/g and 20.14 mg GAE/g, respectively, in the stem hot water extract. ABTS and DPPH radical scavenging activities were 541.1±21.0 and 649.5±6.6 ㎍/mL, respectively, in stem hot water extract. All extracts were non-cytotoxic in HepG2 cells, but 200 ㎍/mL stem extracts tended to decrease the viability of RAW 264.7 cells. The highest xanthine oxidase inhibitory activity was 43.68% in the root hot water extract at 200 ㎍/mL. The expression level of MMP-9 was significantly decreased in the asparagus extracts. The highest GGT, AST, and LDH activities showed a concentration-dependent decrease in the stem ethanol extract. In conclusion, the presence of bioactive substances in the non-edible extracts of asparagus was confirmed for the development of extracts with antioxidant, hepatoprotective and anti-gout activities.

본 연구에서는 아스파라거스 부산물인 줄기와 뿌리 추출물의 루틴 및 총폴리페놀 함량, DPPH 및 ABTS radical 소거능, xanthine oxidase 및 MMP-9 저해활성, 간세포 보호효과를 분석하였다. 루틴과 총폴리페놀 함량은 아스파라거스 줄기 열수 추출물 31.74 mg/g과 20.14 mg GAE/g으로 가장 많았으며, ABTS radical 및 DPPH radical 소거능은 페놀성 화합물의 함량이 상대적으로 높은 줄기 열수 추출물에서 IC50 값이 각각 541.1±21.0, 649.5±6.6 ㎍/mL이였으며, 아스파라거스 추출물에 존재하는 페놀성 화합물과 radical 소거능과의 연관성을 시사하였다. Xanthine oxidase 저해활성은 아스파라거스 뿌리 열수추출물이 200 ㎍/mL 농도에서 43.68%의 저해활성을 보였다. HepG2 간세포는 아스파라거스 모든 추출물에서 세포독성이 없는 것으로 나타났으나, RAW 264.7 대식세포의 경우 아스파라거스 줄기 열수 및 20% 에탄올 추출물 200 ㎍/mL 농도에서 각각 90.18, 84.14%로 생존률이 유의하게 감소하는 것으로 나타났다. MMP-9 발현량은 아스파라거스 추출물 처리구에서 전반적으로 감소하였고, 특히 줄기 추출물 처리구에서 MSU 처리구와 비교하여 유의하게 감소하는 경향을 보였다. HepG2 간세포에 3% 에탄올을 처리하여 간독성을 유발하여 GGT, AST 및 LDH 활성을 측정한 결과 아스파라거스 줄기 20% 에탄올 추출물이 가장 우수한 것으로 나타났다. 이 결과를 토대로 아스파라거스 줄기 및 뿌리는 총 폴리페놀과 루틴이 다량 함유되어 있어 생리활성 소재로써 개발 가치가 높을 것으로 판단되며, 추가적인 in vivo 생리활성평가를 통해 활용방안 모색이 필요할 것으로 판단된다.

Keywords

References

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