융합정보논문지 (Journal of Convergence for Information Technology)

중소기업융합학회 (Convergence Society for SMB)
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흑구기자 열매의 생리활성 평가 연구

Antioxidant and Anti-inflammatory Properties of Two Extracts from Lycium ruthenicum Murray Fruit

  • 추결 (서경대학교 일반대학원 미용예술학과) ;
  • 이지안 (서경대학교 일반대학원 미용예술학과)
  • Zou, Jie (Dept. of Beauty Art, Graduate School, Seokyeong University) ;
  • Lee, Ji-An (Dept. of Beauty Art, Graduate School, Seokyeong University)
  • 투고 : 2021.03.16
  • 심사 : 2021.05.20
  • 발행 : 2021.05.28
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초록

본 연구의 목적은 흑구기자의 생리학적 활성을 평가하여 화장품 산업에 활용 가능한 원료로서의 가능성을 조사하고자 흑구기자 열매에 70% 메탄올(LRM)과 열수(LRW)를 이용하여 추출물을 획득하였다. DPPH와 ABTS 라디컬 소거능 결과 LRW 추출물 보다 LRM 추출물에서 라디컬 소거 활성이 증가하였다. FRAP 분석 결과 LRM 추출물에서 LRW 추출물보다 FRAP 값이 1.3배 증가하였다. 총 폴리페놀 함량은 각각 LRM 추출물에서 31.883±1.395 mg/g, LRW 추출물에서 27.748±2.741 mg/g으로 확인되었다. LRM 추출물은 LPS 자극 후 RAW264.7 세포에서 NO 생성을 억제하였다. 또한 LRM 추출물은 LPS에 의한 COX-2, PGE2, IL-6 및 TNF-α의 발현을 저해하였다. 이러한 결과들로 흑구기자 열매는 화장품에 활용되는 천연 항산화 및 항염 물질로서 가능성이 높을 것으로 기대된다.

The aim of the study was to evaluate the physiological activity of Lycium ruthenicum to investigate their potential as a raw material that can be used in the cosmetic industry. L. ruthenicum fruit extracts were obtained using 70% methanol(LRM) and hot-water(LRW). The DPPH and ABTS radical scavenging abilities were higher in the LRM extract than in the LRW extract. The FRAP value of LRM was about 1.3-fold greater than that of LRW. The polyphenol contents of LRM and LRW were 31.883±1.395 mg/g and 27.748±2.741 mg/g respectively. LRM inhibited the generation of NO in LPS-stimulated RAW264.7 cells. LRM also attenuated the expression of COX-2, PGE2, IL-6, and TNF-α induced by LPS. These results suggests that L. ruthenicum fruits could be use as a source of natural antioxidants and anti-inflammatory agent in cosmetic products.

키워드

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