Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Comparison of antioxidant activities of pearled and wholegrain barley harvested in Jeju

도정 유무에 따른 제주산 보리의 항산화 활성 비교

  • Kang, Yuri (Department of Food Bioengineering, Jeju National University) ;
  • Kim, Hyun Jung (Department of Food Bioengineering, Jeju National University)
  • 강유리 (제주대학교 식품생명공학과) ;
  • 김현정 (제주대학교 식품생명공학과)
  • Received : 2021.03.22
  • Accepted : 2021.05.12
  • Published : 2021.06.30
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Abstract

Barley contains health-beneficial compounds, such as phenolic acids, flavonoids, lignans, and tocopherols. The antioxidant activities of pearled and wholegrain hull-less, premature, and black barley harvested in Jeju were investigated by measuring total phenolic content (TPC), total flavonoid content (TFC), phenolic compounds, tocopherols (T), and tocotrienols (T3), along with their in vitro antioxidant activities. Consequently, TPC and TFC in wholegrain barley groups were higher than those in pearled barley. Gallic acid (1.55-2.98 ㎍/g) and protocatechuic acid (0.67-2.84 ㎍/g) were the predominant phenolic compounds in barley. Total T and T3 concentrations of wholegrain barley groups were significantly higher than those of pearled barley (p<0.05). Except for the metal chelating effect and reducing power, the in vitro antioxidant activities of wholegrain barley were significantly higher than those of pearled barley. These results indicate that wholegrain Jeju barley can be used as a natural antioxidant source in the food industry.

본 연구에서는 제주산 쌀보리인 일반보리, 청보리, 흑보리를 이용하여 도정 유무에 따른 항산화 성분과 활성을 비교하였다. 제주산 쌀보리의 항산화 성분인 총 폴리페놀 함량은 도정하지 않은 쌀보리에서 139.99-149.90 ㎍ GAE/g, 도정한 쌀보리는 91.14-125.45 ㎍ GAE/g 범위로 나타나 도정하지 않은 쌀보리에서 보다 높은 총 폴리페놀 함량을 보였다. 또한 총 플라보노이드 함량도 도정하지 않은 쌀보리(0.89-1.01 ㎍ QE/g)가 도정한 쌀보리(0.50-0.54 ㎍ QE/g)보다 높은 함량을 나타내었다. HPLC로 분석한 제주산 쌀보리의 페놀성 화합물로 gallic, protocatechuic, vanillic, p-coumaric 및 ferulic acid가 주로 검출되었으며, 도정하지 않은 흑보리와 도정한 일반보리에서는 p-coumaric acid는 검출되지 않았다. Tocopherols 및 tocotrienols 분석 결과에서는 8가지 이성체 중 α-T3 함량이 높았고, 도정하지 않은 쌀보리의 α-T3는 20.68-33.09 ㎍/g으로 도정한 쌀보리 8.93-14.98 ㎍/g보다 유의적으로 높게 검출되었다. 제주산 쌀보리의 in vitro 항산화 활성 결과에서 금속 이온 제거능을 제외하고 도정하지 않은 쌀보리가 높은 항산화 활성을 나타내었고, 항산화 활성 중 SOD 소거 활성이 높게 나타내었다. In vitro 항산화 활성은 총 폴리페놀 및 총 플라보노이드 함량에 비해 총 tocopherols 및 tocotrienols 함량에 더 높은 상관관계를 보였다. 따라서 도정하지 않은 제주산 쌀보리에서 높은 항산화 성분 및 활성이 나타나 이를 천연 항산화제로 활용 가능할 것이라 생각된다.

Keywords

Acknowledgement

본 논문은 2020학년도 제주대학교 교원성과지원사업에 의해 연구되었으며, 이에 감사드립니다.

References

  1. Abdel-Aal ESM, Choo TM, Dhillon S, Rabalski I. Free and bound phenolic acids and total phenolics in black, blue, and yellow barley and their contribution to free radical scavenging capacity. Cereal Chem. 89: 198-204 (2012) https://doi.org/10.1094/CCHEM-10-11-0116
  2. Baik BK, Ullrich SE. Barley for food: characteristics, improvement, and renewed interest. J. Cereal Sci. 48: 233-242 (2008) https://doi.org/10.1016/j.jcs.2008.02.002
  3. Beak SY, Lee YJ, Jang GY, Kim MY, Oh NS, Lee MJ, Kim HY, Lee JS, Jeong HS. Functional components of barley bran with different particle sizes and cultivars. J. Korean Soc. Food Sci. Nutr. 46: 1171-1177 (2017) https://doi.org/10.3746/jkfn.2017.46.10.1171
  4. Cho SH. Vitamin E: α-tocopherol and the other forms of vitamin E. J. Nutr. Health. 43: 304-314 (2010) https://doi.org/10.4163/kjn.2010.43.3.304
  5. Choi ID, Woo KS, Choi HS, Lee SK, Park JY, Chun A, Han SI, Choi DS, Chun JY. Antioxidant properties, β-carotene and vitamin E of different varieties of brown and white rice. J. Korean Soc. Food Sci. Nutr. 41: 1259-1267 (2018) https://doi.org/10.3746/JKFN.2012.41.9.1259
  6. Do TDT, Cozzolino D, Muhlhausler B, Box A, Able AJ. Antioxidant capacity and vitamin E in barley: Effect of genotype and storage. Food Chem. 187: 65-74 (2015) https://doi.org/10.1016/j.foodchem.2015.04.028
  7. Eo HJ, Kim DS, Kang YG, Kim KO, Park YK, Park GH. Antioxidant and immunoregulatory effects of Korean Rhamnaceae. J. Plant Biotechnol. 47: 254-259 (2020) https://doi.org/10.5010/JPB.2020.47.3.254
  8. Farvin KS, Jacobsen C. Phenolic compounds and antioxidant activities of selected species of seaweeds from Danish coast. Food Chem. 138: 1670-1681 (2013) https://doi.org/10.1016/j.foodchem.2012.10.078
  9. Gong L. Barley. In: Bioactive factors and processing technology for cereal foods. Wang J, Sun B, Tsao R (Eds). Springer, Berlin, Germany, pp. 55-64 (2019)
  10. Ham HM, Woo KS, Lee BW, Park JY, Sim EY, Kim BJ, Lee CW, Kim SJ, Kim WH, Lee JS, Lee YY. Antioxidant compounds and activities of methanolic extracts from oat cultivars. J. Korean Soc. Food Sci. Nutr. 44: 1660-1665 (2015) https://doi.org/10.3746/JKFN.2015.44.11.1660
  11. Heo SJ, Cha SH, Lee KW, Cho SK, Jeon YJ. Antioxidant activities of chlorophyta and phaeophyta from Jeju Island. Algae 20: 251- 260 (2005) https://doi.org/10.4490/ALGAE.2005.20.3.251
  12. Jeong GH, Jeong YH, Nam JH, Kim TH. Characterization of antioxidant constituents from perilla cake. J. Korean Soc. Food Sci. Nutr. 48: 900-906 (2020)
  13. Kang DS, Cho MG. Antioxidant activities of mixed grains. J. Korean Soc. Food Sci. Nutr. 29: 635-642 (2016) https://doi.org/10.9799/ksfan.2016.29.5.635
  14. Kang SM, Song SH. Major components and health functionalities of brown rice, germinated brown rice, barley, and buckwheat. Food Eng. Prog. 20: 175-182 (2016) https://doi.org/10.13050/foodengprog.2016.20.3.175
  15. Kim HJ, Kim HJ. Physicochemical characteristics of Jeju barley and extracted β-glucan. Food Eng. Prog. 19: 132-138 (2015) https://doi.org/10.13050/foodengprog.2015.19.2.132
  16. Kim JH, Kim JH, Lee SJ, Hong KW, Kwon YA, Park JC, Kim WJ. Characterization of fermentation kinetics of beer made of Korean 6 row-barley. Food Eng. Prog. 17: 1-9 (2013)
  17. Kim KM, Kang CS, Kim YK, Kim KH, Park JH, Yoon YM, Park HH, Jeong HY, Choi CH, Park JH, Kim YJ, Chenog YK. Past and current status, and prospect of winter cereal crops research for food and forage in Korea. Korean J. Breed. Sci. 52: 73-92 (2020) https://doi.org/10.9787/kjbs.2020.52.s.73
  18. Kim EH, Lee YJ, Jang GY, Kim MY, Yoon NR, Ji YM, Lee MJ, Lee JS, Jeong HS. Functional components of different varieties of barley powder with varying degrees of milling. Korean J. Food Sci. Technol. 48: 256-261 (2016) https://doi.org/10.9721/KJFST.2016.48.3.256
  19. Ko SN, Kim CJ, Kim H, Kim CT, Chung SH, Tae BS, Kim IH. Tocol levels in milling fractions of some cereal grains and soybean. J. Am. Oil Chem. Soc. 80: 585-589 (2003) https://doi.org/10.1007/s11746-003-0742-9
  20. Kong SH, Choi YM, Lee SM, Lee JS. Antioxidant compounds and antioxidant activities of the methanolic extracts from milling fractions of black rice. J. Korean Soc. Food Sci. Nutr. 37: 815-819 (2008) https://doi.org/10.3746/JKFN.2008.37.7.815
  21. KOSTAT. Food grain consumption survey report. Statistics Korea (KOSTAT). Daejeon, Korea. pp. 26-27 (2020)
  22. Lee JW, DO JH, Shim KH. Antioxidant activity of the water soluble browning reaction products isolated from Korean red ginseng. DPPH radical and hydrogen peroxide scavenging. J. Ginseng Res. 23: 176-181 (1999)
  23. Lee JH, Kim HJ, Jee YH, Jeon YJ, Kim HJ. Antioxidant potential of Sargassum horneri extract against urban particulate matter-induced oxidation. Food Sci. Biotechnol. 29: 855-865 (2020) https://doi.org/10.1007/s10068-019-00729-y
  24. Lee TH, Kim HJ, Kim YB. Depigmentation activity of barley, unpolished rice, Job's-tear. J Korean Med. Ophthalmol. Otolaryngol. Dermatol. 16: 57-78 (2003)
  25. Lee MJ, Kim SH, Kim HS, Kim HY, Seo WD, Choi SW, Lee KS, Jang KC. Production of γ-aminobutyric acid using the Korean hull-less barley bran with glutamate. Korean J. Crop. Sci. 63: 35- 40 (2018) https://doi.org/10.7740/KJCS.2018.63.1.035
  26. Lee MJ, Kim YK, Lee YY, Kim HS, Choi SK, Lee KS, Seo WD, Kang HJ, Park KD. Physicochemical properties and antioxidant activity of cooked rice added with Korean naked oat (Avena Sativa L.). Food Eng. Prog. 20: 328-333 (2016) https://doi.org/10.13050/foodengprog.2016.20.4.328
  27. Lee KH, Senevirathne M, Ahn CB, Je JY. Biological compounds extracted from codium fragile by enzymatic hydrolysis and their biological activities. J. Korean Soc. Food Sci. Nutr. 39: 953-959 (2010) https://doi.org/10.3746/JKFN.2010.39.7.953
  28. Lee SK, Woo KS, Lee HL, Lee JH, Lee BW, Lee YY, Lee BK, Kim SL, Kim HJ. Evaluation of antioxidant and tyrosinase inhibitory activities of barley by cultivars and milling recovery. J. Korean Soc. Food Sci. Nutr. 46: 1556-1560 (2017) https://doi.org/10.3746/jkfn.2017.46.12.1556
  29. Lee SM, You YH, Kim KM, Park JJ, Jeong CS, Jhon DY, Jun WJ. Antioxidant activities of native Gwangyang Rubus coreanus Miq. J. Korean Soc. Food Sci. Nutr. 41:327-332 (2012) https://doi.org/10.3746/JKFN.2012.41.3.327
  30. Lopez-Perea P, Guzman-Ortiz FA, Roman-Gutierrez AD, Castro-Rosas J, Gomez-Aldapa C A, Rodriguez-Marin ML, Torruco-Uco JG. Bioactive compounds and antioxidant activity of wheat bran and barley husk in the extracts with different polarity. Int. J. Food Prop. 22: 646-658 (2019) https://doi.org/10.1080/10942912.2019.1600543
  31. Park SM, Choi YM, Kim YH, Ham HM, Jeong HS, Lee JS. Antioxidant content and activity in methanolic extracts from colored barley. J. Korean Soc. Food Sci. Nutr. 40: 1043-1047 (2011) https://doi.org/10.3746/JKFN.2011.40.7.1043
  32. Park YJ, Sung JH, Choi YM, Kim YH, Kim MH, Jeong HS, Lee JS. Analysis of vitamin E in agricultural processed foods in Korea. J. Korean Soc. Food Sci. Nutr. 45: 771-777 (2016) https://doi.org/10.3746/JKFN.2016.45.5.771
  33. Seo SJ, Choi YM, Lee SM, Kong SH, Lee JS. Antioxidant activities and antioxidant compounds of some specialty rices. J. Korean Soc. Food Sci. Nutr. 37: 129-135 (2008) https://doi.org/10.3746/JKFN.2008.37.2.129
  34. Sim U, Lee SE, Lee SH, Choi YM, Lee JS. Change in vitamin E and K contents and true retention of cereal and legume by cooking. J. Korean Soc. Food Sci. Nutr. 47: 675-681 (2018) https://doi.org/10.3746/jkfn.2018.47.6.675
  35. Sung HM, Seo YS, Yang EJ. Anti-oxidant and anti-inflammatory activities of hot water extract obtained from Geranium thunbergii using different extraction temperatures and times. J. Korean Soc. Food Sci. Nutr. 47: 1006-1013 (2018) https://doi.org/10.3746/jkfn.2018.47.10.1006
  36. Yi MR, Hwang JH, Oh YS, Oh HJ, Lim SB. Quality characteristic and antioxidant activity of Immature Citrus unshiu vinegar. J. Korean Soc. Food Sci. Nutr. 43: 250-257 (2014) https://doi.org/10.3746/JKFN.2014.43.2.250
  37. Zhao H, Fan W, Dong J, Lu J, Chen J, Shan L, Lin Y, Kong W. Evaluation of antioxidant activities and total phenolic contents of typical malting barley varieties. Food Chem. 107: 296-304 (2008) https://doi.org/10.1016/j.foodchem.2007.08.018