Mycobiology

  • 제49권2호
  • /
  • Pages.95-104
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  • 2021
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  • 1229-8093(pISSN)
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  • 2092-9323(eISSN)
한국균학회 (The Korean Society of Mycology)
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Genetic Manipulation and Transformation Methods for Aspergillus spp.

  • Son, Ye-Eun (School of Food Science and Biotechnology, Kyungpook National University) ;
  • Park, Hee-Soo (School of Food Science and Biotechnology, Kyungpook National University)
  • 투고 : 2020.08.24
  • 심사 : 2020.10.13
  • 발행 : 2021.04.30
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초록

Species of the genus Aspergillus have a variety of effects on humans and have been considered industrial cell factories due to their prominent ability for manufacturing several products such as heterologous proteins, secondary metabolites, and organic acids. Scientists are trying to improve fungal strains and re-design metabolic processes through advanced genetic manipulation techniques and gene delivery systems to enhance their industrial efficiency and utility. In this review, we describe the current status of the genetic manipulation techniques and transformation methods for species of the genus Aspergillus. The host strains, selective markers, and experimental materials required for the genetic manipulation and fungal transformation are described in detail. Furthermore, the advantages and disadvantages of these techniques are described.

키워드

과제정보

This research was supported by the National Research Foundation of Korea (NRF) grant to HSP funded by the Korean government (NRF-2020R1C1C1004473).

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피인용 문헌

  1. Inducible Selectable Marker Genes to Improve Aspergillus fumigatus Genetic Manipulation vol.7, pp.7, 2021, https://doi.org/10.3390/jof7070506