Journal of Convergence for Information Technology (융합정보논문지)

Convergence Society for SMB (중소기업융합학회)
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Antioxidative Effect of Stachys japonica Miq Extract on Cultured NIH3T3 Fibroblasts Damaged by Ferrous chloride, Mordant

염화제일철 매염제로 손상된 배양 NIH3T3 섬유아세포에 대한 석잠풀 추출물의 항산화 효과

  • Sohn, Young-Woo (Divsion of Medicine School, Sanbon Hospital, Wonkwang University) ;
  • Yoo, Sun-Mi (Dept. of Beauty & Cosmetics, Wonkwang Health Science University)
  • 손영우 (원광대학교 의과대학 산본병원) ;
  • 유선미 (원광보건대학교 미용피부화장품과)
  • Received : 2021.06.03
  • Accepted : 2021.08.20
  • Published : 2021.08.28
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Abstract

The aim of this study was to examined the dermatoxicity of ferrous chloride (FeCl2) and the antioxidative effect of Stachys japonica Miq (SJ) extract on FeCl2-induced cytotoxicity. For this study, superoxide anion-radical (SAR)-scavenging and superoxide dismutase (SOD)-like abilities with cell viability were done. FeCl2 showed a significant decrease of cell viability in dose-dependent manner, and it was mid-toxic. The caffeic acid showed a significant increase of cell viability against FeCl2-induced cytotoxicity. In the protective effect of SJ extract on FeCl2-induced cytotoxicity, it showed SAR-scavenging and SOD-like abilities with a significant increase of cell viability. From these results, the cytotoxicity of FeCl2 is correlated with oxidative stress, and SJ extract effectively protected the cytotoxicity of FeCl2 by antioxidative effect. Conclusively, the natural resources like SJ extract may be a useful fundamental materials for the development of an alternative antioxidant.

본 연구 목적은 염화제일철(FeCl2)의 세포독성과 석잠풀(SJ) 추출물의 항산화 효과 및 FeCl2의 독성을 산화적 손상 측면에서 조사하였다. 이를 위해, 세포 생존율을 비롯한 과산소음이온(SAR)-소거능과 슈퍼옥사이드(SOD)-유사 활성능을 분석하였다. 본 연구에서 FeCl2는 처리 농도에 비례하여 유의한 세포 생존율의 감소를 보였으며, 중간 독성으로 나타났다. 또한, caffeic acid는 FeCl2로 감소 된 세포 생존율에 대해 유의한 증가를 보였다. FeCl2의 독성에 대한 SJ 추출물의 처리는 세포 생존율의 유의한 증가와 함께 SAR-소거 능과 SOD-유사 활성 능과 같은 항산화 효과를 보였다. 결론적으로, SJ 추출물과 같은 천연소재는 항산화제와 같은 대체물질 개발에 있어 기초자료로서의 활용적 가치가 클 것으로 생각된다.

Keywords

Acknowledgement

This paper was supported by Wonkwang University in 2021

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