Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Influence of Allyl Isothiocyanate on the Soil Microbial Community Structure and Composition during Pepper Cultivation

  • Gao, Jingxia (Institute of Germplasm Resources, Ningxia Academy of Agriculture and Forestry Sciences) ;
  • Pei, Hongxia (Institute of Germplasm Resources, Ningxia Academy of Agriculture and Forestry Sciences) ;
  • Xie, Hua (Institute of Germplasm Resources, Ningxia Academy of Agriculture and Forestry Sciences)
  • Received : 2020.12.09
  • Accepted : 2021.03.29
  • Published : 2021.07.28
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Abstract

Allyl isothiocyanate (AITC), as a fumigant, plays an important role in soil control of nematodes, soil-borne pathogens, and weeds, but its effects on soil microorganisms are unclear. In this study, the effects of AITC on microbial diversity and community composition of Capsicum annuum L. soil were investigated through Illumina high-throughput sequencing. The results showed that microbial diversity and community structure were significantly influenced by AITC. AITC reduced the diversity of soil bacteria, stimulated the diversity of the soil fungal community, and significantly changed the structure of fungal community. AITC decreased the relative abundance of dominant bacteria Planctomycetes, Acinetobacter, Pseudodeganella, and RB41, but increased that of Lysobacter, Sphingomonas, Pseudomonas, Luteimonas, Pseudoxanthomonas, and Bacillus at the genera level, while for fungi, Trichoderma, Neurospora, and Lasiodiplodia decreased significantly and Aspergillus, Cladosporium, Fusarium, Penicillium, and Saccharomyces were higher than the control. The correlation analysis suggested cellulase had a significant correlation with fungal operational taxonomic units and there was a significant correlation between cellulase and fungal diversity, while catalase, cellulose, sucrase, and urease were the major contributors in the shift of the community structure. Our results will provide useful information for the use of AITC in the assessment of environmental and ecological security.

Keywords

Acknowledgement

This study was supported by the fund projects: The Whole Industrial Chain Innovation Demonstration Project (NGST2021-8-5) and the National Technical System of Bulk Vegetable Industry (cars-23-g24). We thank LetPub (www.letpub.com) for its linguistic assistance during the preparation of this manuscript.

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