Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
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Exposure of chlorpyrifos impairs the normal function of boar spermatozoa

  • Adikari Arachchige Dilki, Indrachapa Adikari (Department of Agricultural Education, College of Education, Sunchon National University) ;
  • Young-Joo, Yi (Department of Agricultural Education, College of Education, Sunchon National University)
  • Received : 2022.04.22
  • Accepted : 2022.05.16
  • Published : 2022.06.01
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Abstract

The misuse of pesticides has resulted in environmental pollution, which directly or indirectly affects all life on earth. Chlorpyrifos is a chlorinated organophosphorus pesticide that is commonly used in agriculture. The aim of this study was to investigate the effects of chlorpyrifos on the fertilization function of boar spermatozoa. Sperm samples from boars were subjected to varying concentrations of chlorpyrifos from 10 to 200 µM for two incubation periods, 30 min or 2 hrs. The boar spermatozoa were then evaluated for motility, motion kinematics, viability, acrosome integrity, chromatin stability, and generation of intracellular reactive oxygen species (ROS). There was a significant percentage reduction in sperm motility and motion kinematic parameters after both incubation periods (p < 0.05). The proportion of viable spermatozoa decreased after incubation for 30 min and 2 hrs in a dose-dependent manner (p < 0.05). A significantly lower percentage of normal acrosomes was observed in spermatozoa exposed to 200 µM chlorpyrifos over both incubation periods, compared to the controls. The damage to sperm DNA was significantly higher when the exposure time to chlorpyrifos was longer. There was a significant increase in the ROS levels in spermatozoa incubated with chlorpyrifos for 2 hrs (p < 0.05). From the results of the present study, it is concluded that direct exposure of boar spermatozoa to chlorpyrifos altered boar sperm characteristics, suggesting potential toxicity that may affect the male reproductive function.

Keywords

Acknowledgement

This work was supported by a Research Promotion Program of Sunchon National University.

References

  1. Abdel-Razik RK, Mosallam EM, Hamed NA, Badawy ME, Abo-El-Saad MM. 2021. Testicular deficiency associated with exposure to cypermethrin, imidacloprid, and chlorpyrifos in adult rats. Environmental Toxicology and Pharmacology 87:103724. https://doi.org/10.1016/j.etap.2021.103724
  2. Adedara IA, Owoeye O, Ajayi BO, Awogbindin IO, Rocha JB, Farombi EO. 2018. Diphenyl diselenide abrogates chlorpyrifos-induced hypothalamic-pituitary-testicular axis impairment in rats. Biochemical and Biophysical Research Communications 503:171-176. https://doi.org/10.1016/j.bbrc.2018.05.205
  3. Adikari AADI, Chandanee MR, Kim BY, Yi YJ. 2022. Fipronil impairs the fertilization competence of boar spermatozoa. Korean Journal of Agricultural Science 49:103-112. https://doi.org/10.7744/KJOAS.20220007
  4. Alaa-Eldin EA, El-Shafei DA, Abouhashem NS. 2017. Individual and combined effect of chlorpyrifos and cypermethrin on reproductive system of adult male albino rats. Environmental Science and Pollution Research 24:1532-1543. https://doi.org/10.1007/s11356-016-7912-6
  5. Babazadeh M, Najafi G. 2017. Effect of chlorpyrifos on sperm characteristics and testicular tissue changes in adult male rats. Veterinary Research Forum 8:319-326.
  6. Berger T. 1990. Pisum sativum agglutinin used as an acrosomal stain of porcine and caprine sperm. Theriogenology 33:689-695. https://doi.org/10.1016/0093-691X(90)90546-6
  7. Bose S, Kumar PS, Vo DVN. 2021. A review on the microbial degradation of chlorpyrifos and its metabolite TCP. Chemosphere 283:131447. https://doi.org/10.1016/j.chemosphere.2021.131447
  8. Buratti FM, Volpe MT, Meneguz A, Vittozzi L, Testai E. 2003. CYP-specific bioactivation of four organophosphorothioate pesticides by human liver microsomes. Toxicology and Applied Pharmacology 186:143-154. https://doi.org/10.1016/S0041-008X(02)00027-3
  9. Chen R, Cui Y, Zhang X, Zhang Y, Chen M, Zhou T, Lan X, Dong W, Pan C. 2018. Chlorpyrifos induction of testicular-cell apoptosis through generation of reactive oxygen species and phosphorylation of AMPK. Journal of Agricultural and Food Chemistry 66:12455-12470. https://doi.org/10.1021/acs.jafc.8b03407
  10. Dutta AL, Sahu CR. 2013. Emblica officinalis Garten fruits extract ameliorates reproductive injury and oxidative testicular toxicity induced by chlorpyrifos in male rats. SpringerPlus 2:541. https://doi.org/10.1186/2193-1801-2-541
  11. ElMazoudy RH, Attia AA, El-Shenawy NS. 2011. Protective role of propolis against reproductive toxicity of chlorpyrifos in male rats. Pesticide Biochemistry and Physiology 101:175-181. https://doi.org/10.1016/j.pestbp.2011.09.003
  12. Hassan MA, El Bohy KM, El Sharkawy NI, Imam TS, El-Metwally AE, Hamed Arisha A, Mohammed HA, Abd-Elhakim YM. 2021. Iprodione and chlorpyrifos induce testicular damage, oxidative stress, apoptosis and suppression of steroidogenic-and spermatogenic-related genes in immature male albino rats. Andrologia 53:e13978.
  13. Joshi SC, Mathur R, Gulati N. 2007. Testicular toxicity of chlorpyrifos (an organophosphate pesticide) in albino rat. Toxicology and Industrial Health 23:439-444. https://doi.org/10.1177/0748233707080908
  14. Koshlukova SE, Reed NR. 2014. Cyclodienes. Encyclopedia of toxicology: Third edition. pp. 1100-1102. Elsevier, Amsterdam, Netherland.
  15. Li J, Pang G, Ren F, Fang B. 2019. Chlorpyrifos-induced reproductive toxicity in rats could be partly relieved under highfat diet. Chemosphere 229:94-102. https://doi.org/10.1016/j.chemosphere.2019.05.020
  16. Mandal TK, Das NS. 2012. Testicular gametogenic and steroidogenic activities in chlorpyrifos insecticide-treated rats: A correlation study with testicular oxidative stress and role of antioxidant enzyme defence systems in SpragueDawley rats. Andrologia 44:102-115. https://doi.org/10.1111/j.1439-0272.2010.01110.x
  17. Martins CF, Dode MN, Bao SN, Rumpf R. 2007. The use of the acridine orange test and the TUNEL assay to assess the integrity of freeze-dried bovine spermatozoa DNA. Genetic and Molecular Research 6:94-104.
  18. Pursel VG, Johnson LA. 1976. Frozen boar spermatozoa: Methods of thawing pellets. Journal of Animal Science 42:927-931.
  19. Rosenfeld PE, Feng L. 2011. Risks of hazardous wastes. Elsevier, Amsterdam, Netherland.
  20. Sai L, Li X, Liu Y, Guo Q, Xie L, Yu G, Bo C, Zhang Z, Li L. 2014. Effects of chlorpyrifos on reproductive toxicology of male rats. Environmental Toxicology 29:1083-1088. https://doi.org/10.1002/tox.21838
  21. Salazar-Arredondo E, de Jesus Solis-Heredia M, Rojas-Garcia E, Hernandez-Ochoa I, Quintanilla-Vega B. 2008. Sperm chromatin alteration and DNA damage by methyl-parathion, chlorpyrifos and diazinon and their oxon metabolites in human spermatozoa. Reproductive Toxicology 25:455-460. https://doi.org/10.1016/j.reprotox.2008.05.055
  22. Tanvir EM, Afroz R, Chowdhury MAZ, Gan SH, Karim N, Islam MN, Khalil MI. 2016. A model of chlorpyrifos distribution and its biochemical effects on the liver and kidneys of rats. Human and Experimental Toxicology 35:991-1004. https://doi.org/10.1177/0960327115614384
  23. Ventura C, Nieto MRR, Bourguignon N, Lux-Lantos V, Rodriguez H, Cao G, Randi A, Cocca C, Nunez M. 2016. Pesticide chlorpyrifos acts as an endocrine disruptor in adult rats causing changes in mammary gland and hormonal balance. The Journal of Steroid Biochemistry and Molecular Biology 156:1-9. https://doi.org/10.1016/j.jsbmb.2015.10.010
  24. Zhang X, Cui W, Wang KE, Chen R, Chen M, Lan K, Wei Y, Pan C, Lan X. 2020. Chlorpyrifos inhibits sperm maturation and induces a decrease in mouse male fertility. Environmental Research 188:109785.  https://doi.org/10.1016/j.envres.2020.109785