The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
권호 표지
DOI QR코드

DOI QR Code

Isolating and characterizing the unrecorded Wild Yeasts from Seawater and Soil in Haeundae and Mongdol Beaches on the Southern Coast of, Korea

남해안 해운대와 몽돌 해수욕장 주변환경으로부터 야생 효모의 분리 및 국내 미기록 효모들의 균학적 특성

  • Seon-Jeong Park (Faculty of Biomedicine and Biotechnology, Paichai University) ;
  • Ji-Eun Jang (Faculty of Biomedicine and Biotechnology, Paichai University) ;
  • Jeong-Su Moon (Faculty of Biomedicine and Biotechnology, Paichai University) ;
  • Hyang-Burm Lee (Department of Agricultural Biological Chemistry, Chonnam National University) ;
  • Jong-Soo Lee (Faculty of Biomedicine and Biotechnology, Paichai University)
  • 박선정 (배재대학교 바이오의약학부) ;
  • 장지은 (배재대학교 바이오의약학부) ;
  • 문정수 (배재대학교 바이오의약학부) ;
  • 이향범 (전남대학교 응용생물공학부) ;
  • 이종수 (배재대학교 바이오의약학부)
  • Received : 2022.01.07
  • Accepted : 2022.01.28
  • Published : 2022.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

This study aimed to isolate wild yeasts from seawaters and soils samples of the Haeundae and Mongdol beaches on the southern coast of Korea, and to characterize these unrecorded wild yeast strains. In total, 41 strains, representing 37 different species of wild yeast were isolated from 70 samples collected from the beaches. Among these, 14 strains were isolated from the alkalophilic medium of yeast extract-peptone-dextrose (YPD) medium (pH 9.0), and 27 strains were isolated concurrently on general YPD medium (pH 6.5). Among the 41 isolated wild yeast strains, Candida insectorum HUD 16-3(JSL-KSS-002) and Metschnikowia citriensis HUD 12-5(JSL-KSS-001) had not previously been recorded. We investigated the microbiological characteristics of these two unrecorded yeast strains and three other strains-, Cystobasidium lysinophilum JSC 52-2(JSL-GGU-019), Candida takata NMD 11-1(JSL-GGU-017) and Candida panamensis ASG 58M-2(JSL-GGU-018) from Jangseoncheon in Jellabuk-do and Jangtaesan in Deajeon city. All five previously unrecorded yeasts were oval and did not form spores. All strains grew well in YPD and yeast extract-malt extract media in a vitamin-free medium. Two strains, including C. insectorum HUD 16-3(JSL-KSS-002) grew well in a 15% NaCl-containing YPD medium. Three strains, including Cys. lysinophilum JSC52-2(JSL-GGU-019) assimilated lactose, and all strains assimilated starch.

본 연구는 남해안 해운대와 인근 몽돌해수욕장 주변의 야생 효모 종 분포특성을 알아보기위해 이들 지역 주변의 물과 모래, 토양 등 70점의 시료들로부터 일반 효모와 호알칼리성 균주 등 모두 37종, 41균주의 야생 효모들을 분리하였다. 이들 야생 효모들 중 Cys. insectorum HUD16-3 (JSLKSS-002)와 M. citriensis HUD12-5 (JSL-KSS-001)등 두 균주들이 국내 보고되지 않은 미기록 효모들로 선별되었고 필자 등이 전라북도 장선천 주변 토양에서 분리하여 국내 미기록 균주로 선별한 Cys. lysinophilum JSC 52-2 (JSL-GGU-019)와 대전광역시 장태산 호수 주변에서 분리하여 선별한 C. takata NMD11-1 (JSL-GGU-017), C. panamensis ASG58M-2 (JSL-GGU-018)등 5종의 미기록 야생 효모들의 균학적 특성을 조사하였다. 이들 미기록 효모들은 모두 난형으로 출아에 의해 영양 증식을 하였고 포자와의 균사를 형성하지 않았다. 미기록 효모 모두 vitamin-free배지에서도 생육하였고 M. citriensis HUD12-5균주는 50% 포도당을 함유한 YPD배지에서 생육하는 강한 내당성을 보였고 Cys. insectorum HUD16-3과 M. citriensis HUD12-5균주들은 15% NaCl을 함유한 YPD배지에서도 생육이 비교적 양호한 호염성균이었다 또한, 미기록 효모들 모두 포도당과 fructose, sucrose, 전분 등을 자화시켰고 특히Cys. lysinophilum JSC 52-2등 3균주들은 lactose를 자화시켰다.

Keywords

Acknowledgement

This work was supported by a grant from the Nakdonggang National Institute of Biological Resources (NNIBR, project No: NNIBR202001207), funded by the Ministry of Environment (MOE) of the Republic of Korea.

References

  1. Lee JS, Choi YJ, Kwon SJ, Yoo JY, Chung DH. Screening and characterization of osmotolerant and gas-producing yeasts from traditional Doenjang and Kochujang. Food Biotechnol 1996;5:54-8.
  2. Lee JS, Lee SH, Kwon SJ, Ahn C, Yoo JY. Enzyme activities and physiological functionality of yeasts from traditional Meju. Kor J Appl Microbiol Biotechnol 1997;25:448-53.
  3. Kim JH, Kim NM, Lee JS. Physiological characteristics and ethanol fermentation of thermotolerant yeast Saccharomyces cerevisiae OE from traditional meju. Kor J Food Nutr 1999;12:490-5.
  4. Kim JS, Lee M, Kim JY, Heo J, Kwon SW, Yun BS, Kim SJ. Distribution and species diversity of wild yeasts isolated from flowers in Korea. Kor J Mycol 2020;48:475-84.
  5. Min JH, Ryu JJ, Kim HK, Lee JS. Isolataion and identification of yeasts from wild flowers in Gyejoksan, Oseosan and Baekamsan of Korea. Kor J Mycol 2013;41:47-51. https://doi.org/10.4489/KJM.2013.41.1.47
  6. Hyun SH, Mun HY, Lee HB, Kim HK, Lee JS. Isolation of yeasts from wild flowers in Gyonggi-do province and Jeju island in Korea and the production of anti-gout xanthine oxidase inhibitor. Kor J Microbiol Biotechnol 2013;41:383-90. https://doi.org/10.4014/kjmb.1309.09002
  7. Hyun SH, Lee JK, Park WJ, Kim HK, Lee JS. Isolation and diversity of yeasts from fruits and flowers of orchard in Sinam-myeon of Yesan-gun, Chungcheongnam-do, Korea. Kor J Mycol 2014;42:21-7.
  8. Hyun SH, Min JH, Lee HB, Kim HK, Lee JS. Isolation and diversity of yeasts from wild flowers in Ulleungdo and Yokjido, Korea. Kor J Mycol 2014;42:28-33. https://doi.org/10.4489/KJM.2014.42.1.28
  9. Hyun SH, Min JH, Kim SA, Lee JS, Kim HK. Yeasts associated with fruits and blossoms collected from Hanbat arboretum, Daejeon, Korea. Kor J Mycol 2014;42:178-82. https://doi.org/10.4489/KJM.2014.42.2.178
  10. Hyun SH, Han SM, Lee JS. Isolation and physiological functionality of yeasts from wild flowers in Seonyudo of Gogunsanyeoldo, Jeollabuk-do, Korea. Kor J Mycol 2014;42:201-6. https://doi.org/10.4489/KJM.2014.42.3.201
  11. Han SM, Hyun SH, Lee JS. Isolation and identification of yeasts from wild flowers in Deogyu mountain and their physiological functionalities. Kor J Mycol 2015;43:47-52. https://doi.org/10.4489/KJM.2015.43.1.47
  12. Han SM, Hyun SH, Lee HB, Lee HW, Kim HK, Lee JS. Isolation and identification of yeasts from wild flowers collected around Jangseong lake in Jeollanam-do, Republic of Korea, and characterization of the unrecorded yeast Bullera coprosmaensis. Mycobiol 2015;43:266-71. https://doi.org/10.5941/MYCO.2015.43.3.266
  13. Han SM, Han JW, Bae SM, Park WJ, Lee JS. Isolation and identification of wild yeasts from soils of paddy fields in Daejeon metropolitan city and Chungcheongnam-do, Korea. Kor J Mycol 2016;44:1-7. https://doi.org/10.4489/KJM.2016.44.1.1
  14. Han SM, Lee JS. Isolation and identification of wild yeasts from soils of an herb park in Seoul metropolitan city and characteristics of unrecorded yeasts. Kor J Mycol 2016;44:108-12.
  15. Han SM, Lee SY, Kim HK, Lee JS. Characterization of wild yeasts Isolated from leaves obtained from Mt. Daedun and Mt. Chilgap, Korea. Kor J Mycol 2017;45:31-42.
  16. Han SM, Kim HK, Lee HB, Lee JS. Isolation and identification of wild yeasts from freshwaters and soils of Nakdong and Yeongsan river, Korea, with characterization of two unrecorded yeasts. Kor J Mycol 2016;44:350-4.
  17. Han SM, Kim JY, Lee HB, Kim HK, Lee JS. Isolation and characterization of wild yeasts from water and riverside soils of Geumgang midstream in Gongju City, Korea. Kor J Mycol 2018;46:98-104.
  18. Kim HK, Kim JY, Han SM, Kim CM, Lee JS. Microbiological characteristics and physiological functionalities of unrecorded wild yeast strains in the soils of Hajodae and Gyungpodae beaches in Korea. Kor J Mycol 2019;47:249-58.
  19. Han SM, Park DJ, Kim JY, Lim HJ, Lee HB, Lee JS. Isolation of wild yeasts obtained from waters and soils of riversides in Sangjubo of Nakdong river and Daechung dam of Geumgang midstream, Korea and characterization of unrecorded wild yeasts. Kor J Mycol 2020;48:237-49.
  20. Park SJ, Jang JE, Lee HB, Lee JS. Isolation of wild yeasts from riversides in Geumgang upstream, Taean and Suncheonman seashores and microbiological characteristics of the unrecorded wild yeasts. Kor J Mycol 2021;49:67-79.
  21. Jang JE, Park SJ, Lee JS. Isolation of wild yeasts and characterization of physiological functionalities of unrecorded wild yeasts obtained from flowers and soils of the Wolpyung park, Daejeon city and Gykpo beach, Buan, Jeollabuk-do in Korea. Kor J Mycol 2021:49:87-100.
  22. Jang JE, Park SJ, Moon JS, Lee HB, Lee JS. Isolation of wild yeasts from freshwaters and soils in Nonsan tream and Sapgyoho in Chungcheongnam-do, Korea, and microbiological characteristics of the unrecorded wild yeasts. Kor J Mycol 2021:49;337-49.
  23. Han SM, Lee SY, Kim HK, Lee JS. Isolation and diversity of wild yeasts from the waters and bank soils of Daejeoncheon, Gapcheon, and Yedeungcheon in Daejeon Metropolitan city, Korea. Kor J Mycol 2017;45:259-69.
  24. Jeon YJ, Park S, Hwang H, Park YH, Cheon W, Goh J, Chung N, Mun HY. Seven yeast strains isolated from freshwaters for the first record in Korea. Kor J Mycol 2020;48:523-31.
  25. Horvath E, Sipiczki M, Csoma H, Miklos I. Yeasts are able to inhibit growth of disease-associated fungi. Research Square 2019. doi.org/10.21203/rs.2.18243/v1
  26. Wnag S, Ruan C, Yi L, Deng L, Yao S, Zeng K. Biocontrol ability and action mechanism of Metschnikowia citriensis against Geotrichum citri-urantii causing sour rot of postharvest citrus fruit. Food Mycol 2020;87:103375.
  27. Tsuji M, Kudoh S. Soil yeasts in the vicinity of Syowa station, east antarctica: their diversity and extracellular enzymes, cold adaptation strategies, and secondary metabolites. Sustainability 2020;12:4518.
  28. Chang CF, Liu YR, Chen SF, Naumov GI, Naumova ES, Lee CF. Five novel species of the anamorphic genus Candida in the Cyberlindnera clade isolated from natural substrates in Taiwan. Antonie van Leeuwenhoek 2012:102,9-21 https://doi.org/10.1007/s10482-012-9708-0