Korean Journal of Acupuncture

Society for Meridian and Acupoint (경락경혈학회)
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Comparative Study of 12 Herbal Formulae Covered by the National Health Insurance Service in Korea

한방건강보험약 12종의 항산화 활성 및 신경세포 독성 스크리닝 연구

  • Seo, Ji Eun (Department of Korean Medical Science, School of Korean Medicine, Pusan National University) ;
  • Lee, Hanul (Department of Korean Medical Science, School of Korean Medicine, Pusan National University) ;
  • Bae, Chang-Hwan (Department of Korean Medical Science, School of Korean Medicine, Pusan National University) ;
  • Yoon, Dong Hak (KyungHee YDH Oriental Clinic) ;
  • Kim, Hee-Young (Korean Medicine Research Center for Healthy Aging, Pusan National University) ;
  • Kim, Seungtae (Department of Korean Medical Science, School of Korean Medicine, Pusan National University)
  • 서지은 (부산대학교 한의학전문대학원 한의과학과) ;
  • 이한울 (부산대학교 한의학전문대학원 한의과학과) ;
  • 배창환 (부산대학교 한의학전문대학원 한의과학과) ;
  • 윤동학 (경희윤동학한의원) ;
  • 김희영 (부산대학교 건강노화한의과학연구센터) ;
  • 김승태 (부산대학교 한의학전문대학원 한의과학과)
  • Received : 2022.03.07
  • Accepted : 2022.03.31
  • Published : 2022.06.27
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Abstract

Objectives : Parkinson's disease (PD) is a neurodegenerative disease caused by dopaminergic neuronal death in the substantia nigra pars compacta. PD is known to be linked with mitochondrial dysfunction and increased oxidative stress. In this study, anti-cytotoxic and anti-oxidative effect of 12 herbal formulae were compared. Methods : According to experts' advice, 12 types of herbal formulae (Gamisoyosan, Galgeuntang, Galgeunhaegitang, Banhabaekchoolcheonmatang, Bojungikgitang, Boheotang, Sihogyejitang, Sihosogantang, Sihocheonggantang, Ojeoksan, Cheongsanggyeontongtang and Palmultang) were selected from 56 types of herbal formulae covered by the National Health Insurance Service in Korea. To detect anti-oxidative effect, 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay was performed, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed to detect anti-cytotoxic effect of 12 herbal formulae using SH-SY5Y human neuroblastoma cells. Results : In DPPH assay, anti-oxidant activity was increased in a dose-dependent manner and half maximal inhibitory concentration was highest in the order of Galgeuntang, Gamisoyosan, Galgeunhaegitang, Ojeoksan, Palmultang, Sihogyejitang, Sihosogantang, Cheongsanggyeontongtang, Sihocheonggantang, Bojungikgitang, Boheotang and Banhabaekchoolcheonmatang. In MTT assay, concentration of 80% cell survival was highest in the order of Sihosogantang, Cheongsanggyeontongtang, Sihocheonggantang, Sihogyejitang, Bojungikgitang, Galgeuntang, Ojeoksan, Boheotang, Palmultang, Galgeunhaegitang, Banhabaekchoolcheonmatang and Gamisoyosan. Formulae with more than 50% DPPH radical scavenging activity at concentrations for 80% cell survival were Sihosogantang, Cheongsanggyeontongtang, Sihogyejitang, Galgeuntang and Sihocheonggantang. Conclusions : Sihosogantang, Cheongsanggyeontongtang, Sihogyejitang, Galgeuntang and Sihocheonggantang extracts can be candidate medicines for PD, but the effect should be validated in PD models.

Keywords

Acknowledgement

This work was supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Instiute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (grant number : HF21C0053).

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